SU950735A1 - Process for producing hyaluronic acid - Google Patents

Description

(54) METHOD FOR OBTAINING HYALUTONIC ACID

one

The invention relates to bnohmin, namely to methods for producing polysaccharides.

A known method for producing hyaluronic acid is that the human umbilical cord, washed with blood, is dehydrated H is degreased with acetone, then crushed in a miter.

The resulting powder is poured with water, stirred and centrifuged. Acetone is added to the supernatant, hyalurn acid is precipitated, which is collected and dried. This treatment gives a yield of 6% gnaluronic acid with a relative viscosity of 1% solution 3.0 1.

The disadvantage of this method is the low yield of the substance and its relatively low viscosity.

The purpose of the invention is to increase the yield of the target product and the viscosity of the solutions obtained from it.

The goal is achieved by the fact that, according to the method of obtaining hyaluronic acid, which consists in cutting the umbilical cord

man, its dehydration, degreasing, subsequent processing with distilled water, centrifugation and isolation of the target product, after processing the raw material with water, the mixture is kept cold, homogenized and the homogenate is heated in a boiling water bath for 5-15 minutes.

The method is carried out as follows.

Claims (1)

A pre-treatment of the umbilical cord of a person is carried out in the following sequence, the umbilical cords are cut into pieces of 10-15 mm and washed from the blood for 4 minutes (washed for not more than 5 minutes). Then, the umbilical cord pieces are placed in cold acetone until they acquire a solid consistency, and then dried under vacuum to constant weight. Next, get hyaluronic acid by the following method. 2 g of dried umbilical chunks are poured with 200 ml of distilled water and left for 10 hours in a refrigerator until the umbilical cords swell. The umbilical cords are homogenized on a homogenizer (MPW-302) two 395 times jfio W min. Then heated in boiling water for a 1K bath for 10 minutes. Then cooled and centrifuged for 15 min at 6000 rpm. Absolute acetone in the ratio 1: 4 is added to the supernatant. After the precipitate has settled down, the acetone is drained, the precipitate is washed three times with 30 ml absolute acetone and dried under vacuum to constant weight. 280 mg of sediment is obtained from 2 g of umbilical cord. This precipitate contains not only hyalonic acid, but also protein, the amount of which is determined according to Lowry. The mass of protein-free sediment, i.e. hyaluronic acid is 205 mg. Thus, out of 2000 m of the half of the box, 205 mg of product is obtained, which is 10.2%. The proposed method of producing hyaluronic acid gives a yield of 10-11% and a relative viscosity of a 0.25% solution equal to 3.0, i.e. the yield of the product increases in comparison with the known 4-5%, and the viscosity of hyaluronic acid obtained by the proposed method increases 4 times. The invention method of obtaining hyaluroic acid, which consists in grinding human umbilical cord, its dehydration, degreasing, subsequent processing with distilled water, centrifugation and separation of the target product, the difference is that processing the raw material with water, the mixture is kept cold, homogenized, and the homogenate is heated in a boiling water bath for 5–15 min. Sources of information taken into account in expert examination 1. Asatiani V. S. Enzyme methods of analysis. M., Science, 1969, p. 566.