SU883720A1 - Method of quantitative determination of tetracyclin and its derivatives - Google Patents
Method of quantitative determination of tetracyclin and its derivatives Download PDFInfo
- Publication number
- SU883720A1 SU883720A1 SU792860558A SU2860558A SU883720A1 SU 883720 A1 SU883720 A1 SU 883720A1 SU 792860558 A SU792860558 A SU 792860558A SU 2860558 A SU2860558 A SU 2860558A SU 883720 A1 SU883720 A1 SU 883720A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- derivatives
- quantitative determination
- butanol
- tetracyclin
- tetracycline
- Prior art date
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Description
II
Изобретение относитс к аналитической химии, а именно, к способам количественного определени тетрациклина и его производных.This invention relates to analytical chemistry, namely, to methods for the quantitative determination of tetracycline and its derivatives.
Известен способ количественного определени тетрациклина и его производных , основанный на спектрофотометрировании анализируемой прюбы в кислой среде 1 .A known method for the quantitative determination of tetracycline and its derivatives, based on spectrophotometry of the analyzed substance in an acidic medium 1.
. Недостатком способа вл етс низка избирательность.. The disadvantage of this method is low selectivity.
Наиболее близким к изобретению по технической сущности и достигаемому результату вл етс способ количественного определени тетрациклина и его производных, заключающийс в последовательной обработке анализируемой пробы нитратом тори и н-бутанолом при рН среды 3,6-,6 с последующим спектрофотбметрированием бутанольного сло 2 .The closest to the invention to the technical essence and the achieved result is a method for quantitative determination of tetracycline and its derivatives, which consists in the sequential processing of the analyzed sample with thorium nitrate and n-butanol at pH 3.6, 6, followed by spectrophotometric measurement of butanol layer 2.
Недостатком способа вл етс егоThe disadvantage of the method is its
сложность, поскольку торий радиоактивен и определение необходимо проводить в специально оборудованных ла боратори х.complexity, since thorium is radioactive and must be determined in specially equipped laboratories x.
Целью изобретени вл етс упрощение определени .The aim of the invention is to simplify the definition.
Поставленна цель достигаетс тем, что согласно способу количественного определени тетрациклина и его производных , обработке н-бутанолом подвергают непосредственно анализируемую пробу и обработку ведут при рН This goal is achieved by the fact that according to the method of quantitative determination of tetracycline and its derivatives, the sample being analyzed is directly treated with n-butanol and the treatment is carried out at pH
10 среды 0-3 в присутствии 1-6 М раствора хлорида натри .10 medium 0-3 in the presence of 1-6 M sodium chloride solution.
Мол рный коэффициент погашени растворов тетрациклинов в н-бутаноле составл ет примерно -см , The molar extinction ratio of tetracycline solutions in n-butanol is about -cm,
IS что отвечает пределу обнаружени 12 мкг/мл дл минимально измер емой оптической плотности 0,03-0,05. При соотношении объемов водной и органической фаз 5:1 предел обнаружени IS which corresponds to a detection limit of 12 µg / ml for a minimum measured absorbance of 0.03-0.05. When the ratio of the volumes of the aqueous and organic phases is 5: 1, the detection limit
20 тетрациклинов из водных растворов составл ет (1-2) -lOS.20 tetracyclines from aqueous solutions are (1-2) -lOS.
Определению тетрациклинов не мешают соли щелочноземельных металловAlkaline earth metal salts do not interfere with the determination of tetracyclines.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU792860558A SU883720A1 (en) | 1979-12-25 | 1979-12-25 | Method of quantitative determination of tetracyclin and its derivatives |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU792860558A SU883720A1 (en) | 1979-12-25 | 1979-12-25 | Method of quantitative determination of tetracyclin and its derivatives |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU883720A1 true SU883720A1 (en) | 1981-11-23 |
Family
ID=20868306
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU792860558A SU883720A1 (en) | 1979-12-25 | 1979-12-25 | Method of quantitative determination of tetracyclin and its derivatives |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU883720A1 (en) |
-
1979
- 1979-12-25 SU SU792860558A patent/SU883720A1/en active
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