SU835395A1 - Plant growth regulator - Google Patents

Description

(54). PLANT GROWTH REGULATOR

The invention relates to chemical means for controlling the growth and development of plants.

The use of 2-chloroethylphosphonic acid as a plant growth regulator 1 is known.

It is known to use as plant growth regulators, in particular, to increase the sugar content in sugar cane, sorghum, and some fruits of 1,2,4-oxadiazinedione-3, 5 derivatives of the general formula

LI 04

to

where R is hydrogen, lower alkyl or .j phenyl; PS is hydrogen or methyl. 21.

However, even the most effective of the known compounds 1-phenyl-6-methyltetrahydro-1, 2,4-oxadiazinedione-3, 5 must be used in high doses: 2-4 kg / ha and higher.

In order to expand the range of compounds with rostregulatory action, as well as their field of application,

to increase efficiency and reduce consumption rates, it was proposed to use as a growth regulator pro-. aqueous tetrahydro-1,2,4-oxadiazinedione-3, 5 of the general formula

ten

where is x

halogen.

- hydrogen, methyl,

nitro group; n 1 or 2;

R is hydrogen, alkyl, aryl; R is hydrogen, C., - C is J-alkyl, aryl, and, if X f, is hydrogen, then R is c-alkyl and / or fiVH; previously used as intermediates for the production of physiologically active substances 3.

The proposed compounds can be obtained by the methods described previously, starting from well-available substances - nitrobenzene, potassium cyanate. and esters of d-halohydric acids. They are u-stable crystalline substances, poorly soluble in water, well in organic solvents, and can be used in the form of emulsions and aqueous dispersions both when soaking seeds, entering into the soil, and during processing during the growing season,

As a method of primary selection of physiologically active compounds, a test is used on cell cultures of higher plants (sugar

beet, wheat, tobacco), which determine the effect of the studied drugs on metabolism and cell growth. The effect on metabolism is determined by the magnitude and change in the intensity of respiration over time.

Variants of the proposed compounds of formula 1 and data on their effect on respiration of suspensions of sugar beet plant cells as a percentage of the control are given in Table 1.

Table 1

nineteen

3,4-C. H

Example 1. A cell culture grown under strictly conditioned conditions (temperature, humidity 70%, modified Schenk and Hildebrandt medium) was used in the screening tests. Tests were carried out at a concentration of substances of 10 mol / l. The readings were taken after 120 hours. The test results were expressed in relative values as a percentage of the control. From a series of 21 preparations; that especially strong regulator. Example 2. The initial callus tissue of wheat (Caucasus variety) was placed in test tubes containing agar medium (according to Murashigi and SkeGU), where the test substance was introduced at a concentration of i mg / l. Growing temperature 26 ° C, humidity 70%. The sizes of calluses after three weeks of growth were determined by weighing. Continuation of the table. one

160-161 176

185

The torus activity was shown. Seven compounds were selected for further testing as plant growth and development regulators. The data of the tests performed are summarized in Table 2.

The growth-regulating activity of the derivatives of osadiaeindions in experiments on wheat germ (data is given as a percentage of the control) is given in Table 2.

table 2

eat. The originality of the experiments is fivefold. Under these conditions, the preparation No. 19-2- (3,4-dichlorophenyl) -6-methyltetrahydro-1, 2,4-ox1 Diazinedione-3.5 accelerates l biomass growth of the illusory tissue is four times more intense than the known auxins, most effective in tissue culture (2,4-DL –NU).

Example 3. The test substance in-concentration of 0.001 and / or 0.01% was introduced in the form of a suspension or solution in a nutrient medium, Knopp, on which wheat seeds were germinated, placed in a tube of filter paper. Further cultivation of seedlings was carried out for 3 weeks with a short light day. 10 days after the start of the experiment, an account was taken of the number of plants in which the second leaf was formed. In X1 variant, five replications with 20 plants each. In one variant of the experiment, the seeds were soaked in a solution of the test substance for 15 hours, after which they were grown on a clean medium for three weeks. The results obtained in this embodiment coincide with the results obtained when introducing substances into the nutrient medium.

Example 4. Intermediate results of case tests of 2-p-tolyl-b-isopropyl tetrahydro-1, 2,4-oxadiazin-3, 5-dione (compound No. 21, Table 1) on rice in the Kuban region.

Rice check area of 8 hectares. Before sowing, the following agrotechnical works were carried out: bb + plowing from bi spring, disking, leveling the plane of the check. 20.5.77 g check covered by seeds of the variety Krasnodar 424 second reproduction, MSh class. The first check fill from 20.5 to 27.5. Discharge and drying was carried out from 27.5 to 4.6. The area of the division is 1 m, the free interval between the branches is 0.5 m. The consumption of grains is 925 pcs. by 1 m. Each variant was performed in 4 times, for 1 m each repetition. Liquid consumption when processing drugs 0.2 liters per 1m.

The results are summarized in table 3.

Sowing

e

Pre-0.25

The same nice addition to

phase 0.50 germination (4 sheets)

Table 3

Example The effect of drugs on the dynamics of the development of rice plants in night experiments.

The conditions of the experiment are similar to example 5. The treatment of seedlings in the 4-leaf phase was performed on 28.6.77 g.

By 6.7, rice on drug treated cases was ahead of the plant in the control by 20 days. In these cases, the tillering phase ended completely, while in the controls only the beginning of this phase was observed. The seedlings of rice, treated before the beginning of the tillering phase (4 leaves) with the drug in doses of 0.5 and 1 kg / ha, for 8 days, after treatment, have completed the passage of this phase, being 15–20 days ahead of the control plants in development.

The result is shown in Table 4. Table 4

5-6

3-4

4-5

6-7

11-12

3-4

10-11

Example 7. Morphological changes in rice under the influence of growth regulators in business experiments.

Results. As of 6.7.77. Sowing and tillage of soil 20.5.77 g. Treatment of seedlings 28.6.77g in phase 4 leaves (% of control). Measurement of 40 plants from each variant (10 plants from the repetition bollard).

Seed locking produces results similar to pre-emergence tillage. Atonic spraying is not recommended as not effective. The change in the thickness of the roots of rice under the action of these drugs was not observed.

Doses of drugs and methods of treatment of them, which gave the greatest effect on each drug, are given in table.5. Under the action of Compound No. 21 (Table 1), the formation of the fibrous root system is observed.

Table 5

Claims (1)

Claim The use of derivatives of tetrahydro ro-1,2,4-oxadiazinedione-3, 5 of the General formula 0 jTVifArB HD = / · I ’gA 'R * where X is hydrogen, methyl, halogen, nitro group; n is 1 or 2; R is hydrogen, alkyl, aryl; 40 R ¹ is hydrogen, C ^ -Cj-alkyl, aryl, and / if X _h is hydrogen, then R? -C-z-alkyl and / or RVH, as a plant growth regulator.