SU786856A3 - Method of preparing escine - Google Patents

Abstract

A method is provided for readily recovering water insoluble crystalline escin as the free acid from seeds of Aesculus hippocastanum, which comprises extracting crushed Aesculus hippocastanum seeds with an organic, water-miscible solvent, preferably a lower aliphatic alcohol,contacting the resulting extraction liquid with a cation exchanger in its H+ form, separating the resulting eluate from said cation exchanger, concentrating the eluate, for example by distillation, and separating the resulting crystalline deposit of escinic acid, for example by centrifuging or other filtering technique the escin is useful in medicine in view of its antiphlogistic action.

Description

The invention relates to the pharmaceutical industry and relates to the preparation of pharmaceutically active substances. A known method of producing escin by extracting horse chestnut seeds (AescuEus hippocastanum) with an aqueous solution of lower alcohols, treating the extract on a cation resin, followed by thickening, purification and extension of the desired product flj. However, the known method does not provide a high activity of the target product. The aim of the invention is higher. the activity of the target product. This goal is achieved by the fact that after treating the extract with a cation, it is concentrated to a ratio of 10: 4, then dried under vacuum, dissolved by heating in 10 times the amount of methanol or ethanol with the addition of 5% activated carbon, then filtered, concentrated to half the volume, add twice the amount of demineralization of bath water with temperature, the resulting solution is cooled to room temperature and, after drying, treated with triethanolamine. Escine is obtained from AescuBus hippocas ta num. The method involves extraction of crushed seeds of AescuEus hippocastanum with a mixture of water with methanol or ethanol containing 65% by volume of alcohol, separating the extraction solution, treating it with an acidic cation exchanger to a pH value of flowing down solution equal to pH 3.5-3.8, thickening the solution, reacting the obtained crystalline product with a base in the medium of a solvent and the separation of the escinate, due to the fact that the concentration of the solution is carried out in a ratio of 10: 4, the resulting crude escin is dried in va-. when dissolved in 10-fold amount of methanol or ethanol, with the addition of 5%, in terms of crude escin, powdered activated carbon, heated under reflux, filtered, the solution is thickened to half, add double the amount of demineralized water with a temperature the solution is brought to room temperature and the resulting crystalline product is dried, followed by a mutual interaction with triethanolamine and isolation of the triethyolamine scinate.

Claims (1)

Claim A method of producing escin by extraction of horse chestnut seeds § (AescuE ^ s hippocastanum) with an aqueous solution of lower alcohols, treatment of the extract 35 with cation exchange resin, followed by thickening it by purification and isolation of the target product, characterized in that, in order to increase the activity of the target product, after processing the extract it is concentrated on a cation exchanger to a ratio of 10: 4, then dried under vacuum at 80 ° C, dissolved by heating in a 10-fold amount of methanol or ethanol with the addition of 5% activated carbon, 45 is further filtered, concentrated to half s volume, add a double amount of demineralized water with a temperature of 90 ° C, the resulting solution is cooled to room temperature and after drying, treated with three ethanolamine.