SU472509A3 - The method of producing polyfructosan - Google Patents

Description

(54) METHOD FOR PRODUCING POLYFRUCTOSANE

This invention relates to the field of medicine and concerns a method for the preparation of diagnostic preparations.

A method of obtaining nolyfructosan, for example, inulin, is that the vegetable raw material is extracted with hot water, the extract is freed from the ballast substances by treatment with basic lead acetate solution, the excess of lead is removed using sodium sulfate solution, the filtrate is evaporated to a thick syrup, it is treated a mixture of activated carbon and silica gel; and inulin was isolated by precipitation with ethyl alcohol. The resulting precipitate is washed with 50% alcohol and dried.

The aim of the proposed invention is to obtain the desired product, effective in the study of the functional capacity of the nights.

To achieve this goal, the extraction is carried out with pulverizing alcohol or acetone containing 40-80% water, the ballast materials are removed by adding calcium hydroxide to pH 9.0, the precipitation is carried out by adding lower alcohol or acetone, bringing the pH to 1.0-3 , 0 and adding an additional amount of lower alcohol or acetone.

2

Example. 100 kg of dried red sea onion are coarsely crushed in a knife mill having a set of sieves with a diameter of 4 mm, then, after pre-wetting, 200 kg of 30% aqueous methanol is kept for 24 hours, after which percol is carried out using the same solvent at room temperature with a speed of 4 kg / h. 300 kg of extract is obtained, which is treated with a suspension of 250 g of calcium hydroxide in a small amount of water to obtain a pH of 9, then the precipitate is filtered off and the liquid is evaporated under vacuum to obtain a thick syrup. Get about 75 kg of syrup with a solids content of 80%. With ehergic stirring, 30 kg of methanol are added, and the almost clear solution is cooled to 15 ° C. Continuing vigorous stirring,

add a cooled mixture consisting of 3 liters of concentrated hydrochloric acid and 8 kg of methanol. Sinistrin precipitates as a viscous, with a matte sheen of yellowish-white mass. Received by

after a brief exposure, the clear yellow solution is allowed to drain completely. It contains salts, fructose, and low molecular weight oligosaccharides. The methanol contained in the solution may

be recovered by distillation and rectification.

The synistric precipitate in an amount of about 40 kg is thoroughly mixed three times in a mixer with methanol (methanol is taken respectively in 40 kg portions). The excess methanol is poured each time and mixed with the methanol obtained during the first precipitation, and at the end the whole mass turns into a fine-grained crystalline suspension, which is centrifuged, to obtain about 35 kg of matter and dissolved in 70 kg of water. The solution is mixed with 3 kg of activated carbon, filtered and passed at a rate of 15 liters / hour through a filter of H-OH ionization, consisting of 5 liters of weakly acidic cation exchanger (H +) and 10 liters of force-bearing anion exchanger (OH). The solution of pure synistrin obtained in this way is concentrated in vacuo to obtain a thick syrup and thin out in steel baths placed in vacuum drying cabinets. The result is a white, fragile, easily sprayed, odorless mass and almost tasteless, capable of dissolving slowly and in any desired ratio in water, but insoluble in most organic solvents, with the exception of pyridine, formamide, butylamine, phenol, acetamide and lower-grade alcohols.

The cryoscopically determined molecular weight is approximately 3,000. The optical activity is -39.2 ° (C

 10, H2O). The yield is 31.5 kg.

Subject invention

The method of obtaining polyfructosan by extracting the original plant raw material, removing the ballast substances from the extract

concentrating the filtrate, precipitating the target product with an organic solvent and purifying it, characterized in that, in order to obtain the desired product, effective in the study of functional

ability of the kidneys, extraction is carried out with lower alcohol or acetone containing 40-80% water, ballast substances are removed by adding calcium hydroxide to pH 9.0, precipitation is carried out by adding

lower alcohol or acetone, adjusting the pH to 1.0-3.0 and adding an additional amount of lower alcohol or acetone.