SU624636A1 - Method of producing k-serum - Google Patents

Description

This invention relates to the field of microbiology.

A known method for obtaining 1K serum by growing a vaccine strain of the genus K bebs efcCoi on a nutrient medium, selecting active colonies followed by re-growing them in a broth with glucose, inactivating the microbial mass obtained with formalin, immunizing animals and isolating the desired product l. However, the determination of the specificity of serum and K-antigen during typing is based on the swelling of the capsule, which introduces additional difficulties both in the production of serums and in practical laboratories, as it is carried out with microscopy of the preparation. Serum may require additional adsorption from O-antibodies.

The aim of the invention is to increase the specificity of the K-serum and to ensure its use in the agglutination reaction.

For this, the cultivation of the vaccine strain is carried out in broth

Hottinger with glucose, and then pass on the agar medium Worfel-Fergusoi and select the active colonies for the formation of a capsule.

The method is as follows.

The vaccine strain is sown in XoTTHi broth with O, 2% glucose for 5-6 hours at 37 ° C, and then peresseivat on

agar medium b (spruce-ferguson, the next day, a cultured preparation is prepared on the arcrit medium of a scrubbing preparation according to burri's method in the modification of labinsky. In the absence of capsules, the passage of the strain is repeated until the preparation is clearly visible outlined capsule. In this case, sowing on nutrient agar in a petri dish is done for viewing on the next

Claims (1)

Day of colonies in a cross-country light along Landy. Colonies of Klebsiel with a capsule have a pink or pink-blue coloring with a grayish-smoky pop-circle in the form of a cap, in the absence of the capsule of the O-shaped colony is orange, gray, beige and smoky semicircle. If there are separate colonies in the O-form in the co-transmitted light, the passaging of the strain is repeated until the entire population of the strain consists of homogeneous colonies in the capsule form of the corresponding color. This strain is used to prepare a vaccine, for which it is grown in Hottinger Boulevard with O, 2% glucose for 4-5 hours, killed by the addition of 0.5% formalin and immunized to rabbits six times in doses of 0.5; 1.0; 2, Of 3.0 | 3.0; 3.0 and exsanguinated 7 days after the last immunization. Prepared sera are controlled by agglutination on glass or in vitro, just like K-anti1 in Klebsiell cultures is determined by the reaction on glass and not by the capsule swelling reaction. Strains with a developed capsule are lofylase to remain in the capsule form e Large mucous membranes of Klebsiella colonies often do not correlate with valic of a pronounced capsule in microbial cells. The invention The method of obtaining K-serum of growing a vaccine strain of the genus KCefasietCa on a nutrient medium, selecting active Colonies, followed by re-growing them in a broth with glucose, inactivating the resulting microbial mass with formalin, immunizing animals and isolating the target product, characterized in that, in order to increase the specificity of the K-serum and the possibility of its use in the agglutination reaction, the cultivation of the vaccine strain is carried out on Hottinger broth with glucose, and then is passaged on an agar medium e VorfelFergyuson and selected active colony formation About capsules. Sources of information taken into account in the examination: l, EdwdHas A-Swihgr.Oden-ki | ication. Of E te oac-fcef iaceae. : r.Minnec (poEls, -JO, - {96, p-AOR.