SU502022A1 - Acid phosphatase release method - Google Patents
Acid phosphatase release methodInfo
- Publication number
- SU502022A1 SU502022A1 SU1898785A SU1898785A SU502022A1 SU 502022 A1 SU502022 A1 SU 502022A1 SU 1898785 A SU1898785 A SU 1898785A SU 1898785 A SU1898785 A SU 1898785A SU 502022 A1 SU502022 A1 SU 502022A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- aspergiilus
- acid phosphatase
- release method
- protein
- extraction
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Description
(54) СПОСОБ ВЫДЕЛЕНИЯ КИСЛОЙ ФОСФАТАЗЫ(54) METHOD FOR ISOLATING ACID PHOSPHATASE
ют. SivCTipaKHHio провод т при 5° с в течааие 1 часв. 0,1 М фосфатном буфере (,рН 7,8). -Твердую фазу отдел ют, -и белок осаждают насыщенным раствором до 80% сернокислым аммонием. Диализи1руют и лиоф.илизируют.yut. SivCTipaKHHio is conducted at 5 ° C for 1 hour. 0.1 M phosphate buffer (pH 7.8). The solid phase is separated, and the protein is precipitated with a saturated solution of up to 80% ammonium sulfate. Dialyzed and lyophilized.
Выход - 0,20 г, активность - 3,6 ед/мг npenaipaTB, 6,5 ед/мг бел«а.The output is 0.20 g, the activity is 3.6 u / mg npenaipaTB, 6.5 u / mg Bel a.
П р и м е р 4. 0,5 кг 8-дневного мнцелк отдел ют от культуральной жидкости к размельчают . Экстрагируют гари 5° С 1 час в фосфатном буфере (1 М), рН 7,5 и осаждают белок этиловым опи1ртом - 50% -к 80%. Ос а док л и оф и л и 3 и р у ю т.PRI me R 4. 0.5 kg of an 8-day multi-compartment is separated from the culture fluid and crushed. Extraction of ashes at 5 ° C for 1 hour in phosphate buffer (1 M), pH 7.5, and precipitate the protein with ethyl alcohol - 50% to 80%. Os doc l and of l and 3 and p y t.
:Выхоа - 0,2 г, активность -1,9 ед1мг препарата, 3,0 ед/мг белка.: Vyhoa - 0.2 g, activity -1.9 u1 mg of the drug, 3.0 u / mg protein.
Пример 5. 0,6 кг 4-днев ного -мицели отдел ют от питательной среды, промывают дза раза водой, а затем деми1нерализоза 1ной водой и гомогенизируют. Экстракци происходит при 5° С в течение 1 час в воде, подК1ислен ной до рН 2,5, и осаждение беЛ1ка ведут пр-и насыщении сернакислым аммоние1м до 50% и 80%. Диализируют и лиофилизируют .Example 5. 0.6 kg of 4-day-wise micelles are separated from the nutrient medium, washed three times with water, and then demineralisosis with 1 water and homogenized. Extraction occurs at 5 ° C for 1 hour in water, adjusted to pH 2.5, and the precipitation of protein is carried out and saturated with ammonium sulfate 1 to 50% and 80%. Dialyzed and lyophilized.
Выход - 0,25 г, а.ктдв1Н.асть-1,5 ед/мг препарата, 2,5 ед/мг белка.The yield is 0.25 g, a.ktdv1.ast-1.5 units / mg drug, 2.5 units / mg protein.
Пр.И|Мер 6. 0,5 кг Ю-днев ного мицели отдел ют от питательной среды « гомогенизируют . Экстракцию провод т в деминерализованной зоде при рН 6,0 и 5° С в течение 1 час. После экстракции довод т рН экстракта до 5,0 11Н NaOH. Белок осаждают до 80% насыщени сухим сернокислым аммонием .Pr.I | Mer 6. 0.5 kg of the 10-day-old mycelium is separated from the nutrient medium and is homogenized. The extraction was carried out in demineralized zod at pH 6.0 and 5 ° C for 1 hour. After extraction, the pH of the extract was adjusted to 5.0 with 11N NaOH. The protein is precipitated to 80% saturation with dry ammonium sulphate.
Диализируют и лиофил.изируют. Выход -0,15 г, а,кти В1Ность - 2,4 ед/мгDialyzed and lyophilized. Output -0.15 g, a, KTI B1Nost - 2.4 U / mg
.препарата, 4,0 ед/мг белка..drug, 4.0 u / mg protein.
Ф О р .М у Л а и 3 о i6 р е т е 1Н и Ф О р .М at Л a and 3 о i6 р е те 1Н and
Claims (2)
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU1898785A SU502022A1 (en) | 1973-03-26 | 1973-03-26 | Acid phosphatase release method |
FR7409536A FR2223384A1 (en) | 1973-03-26 | 1974-03-20 | Acid phosphatase prepn. - by aq. extraction of aspegillus terreus mycelium, fractionation with ammonium sulphate and freeze-drying |
CS218674A CS176503B1 (en) | 1973-03-26 | 1974-03-26 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU1898785A SU502022A1 (en) | 1973-03-26 | 1973-03-26 | Acid phosphatase release method |
Publications (1)
Publication Number | Publication Date |
---|---|
SU502022A1 true SU502022A1 (en) | 1976-02-05 |
Family
ID=20546976
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU1898785A SU502022A1 (en) | 1973-03-26 | 1973-03-26 | Acid phosphatase release method |
Country Status (3)
Country | Link |
---|---|
CS (1) | CS176503B1 (en) |
FR (1) | FR2223384A1 (en) |
SU (1) | SU502022A1 (en) |
-
1973
- 1973-03-26 SU SU1898785A patent/SU502022A1/en active
-
1974
- 1974-03-20 FR FR7409536A patent/FR2223384A1/en active Granted
- 1974-03-26 CS CS218674A patent/CS176503B1/cs unknown
Also Published As
Publication number | Publication date |
---|---|
CS176503B1 (en) | 1977-06-30 |
FR2223384B1 (en) | 1976-06-25 |
FR2223384A1 (en) | 1974-10-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE60320107D1 (en) | PREPARATION OF FERMENTATION PRODUCTS | |
CN110256603A (en) | A kind of-two step enzyme method coupling of shrimp and crab shells hydro-thermal prepares the methods and applications of chitin and chitosan | |
SU502022A1 (en) | Acid phosphatase release method | |
GB1356449A (en) | Process for extracting water soluble components of the hyphae of edible fungi | |
CN103834711B (en) | Preparation of protein hydrolysate of chilled Antarctic krill and application thereof | |
GB1276245A (en) | Enzymatic production of gluconic acid | |
GB852995A (en) | Improvements in or relating to the production of distilled spirits | |
IE39141L (en) | Production of immunological material | |
GB1141821A (en) | A process of producing an active substance for stimulating the function of the reticuloendothelial system | |
US2102315A (en) | Process of preparing an enzyme from mold | |
US2524089A (en) | Process of producing subtilin | |
SU597648A1 (en) | Method of processing excess activated sludge | |
SU940648A3 (en) | Process for producing 4-amino-5-chloro-1-phenylpyrid-azone-(6) | |
GB1362108A (en) | Process for preparing hydroxy-l-proline or n-acetyl-hydroxy- l-proline from hydrolysed gelatin | |
GB1291149A (en) | Method of isolating vitamin b12 from fermentation broth | |
CN106282286A (en) | A kind of extracting method of little molecule shellfish polypeptide | |
GB1036815A (en) | Method of producing n-methyleneglycinonitrile | |
SU603389A1 (en) | Method of preparing sunstance of collagenasic action | |
FR2059471A1 (en) | Heteroploid humas liver epitherial cell - strain as culture for calcination and diagnosis | |
SU548622A1 (en) | Yeast Growing Method | |
GB1197942A (en) | Process for the production of the Antibiotic Pleuromutilin | |
GB1433528A (en) | Method for producing deacetylcephalosporin c | |
GB1325423A (en) | 2-amino-2-aryl-acetic acids and esters thereof | |
Berman et al. | Synthetic carbohydrate: An aid to nutrition in the future[Final Report] | |
GB713795A (en) | Improvements in or relating to terramycin |