SU603389A1 - Method of preparing sunstance of collagenasic action - Google Patents
Method of preparing sunstance of collagenasic actionInfo
- Publication number
- SU603389A1 SU603389A1 SU762400605A SU2400605A SU603389A1 SU 603389 A1 SU603389 A1 SU 603389A1 SU 762400605 A SU762400605 A SU 762400605A SU 2400605 A SU2400605 A SU 2400605A SU 603389 A1 SU603389 A1 SU 603389A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- sunstance
- collagenasic
- preparing
- action
- precipitate
- Prior art date
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Description
(54) СПОСОБ ПОЛУЧЕНИЯ ВЕЩЕСТВА КОЛЛАГЕНАЖ ОГО(54) METHOD OF OBTAINING COLLAGENAGE COGO AGENT
ДЕЙСТВИЯACTIONS
Огпеление ба/гластпых белков осуществл ют осаждонием сернокислым аммонием, Из первого экстракта - при О,35 насышении , а из второго - при 0,3 касьщенин, Осааки отдел ют филыграцией. Из обоих фильтратов осаждают препарат коллагеназногр действи добавлением сернокислого аммони до степей насьпиени О,5-О,6, После этого |)еакционные смеси объедин ют н подвергают отстаиванию в течение 1618 час, вадосадочную жидкость сифонируют , а осадок отдел ют филетрацией под вакуумом. Осадок, содержащий фермент, раствор ют в трех объемах воды к массе осадка, разливают в целлофановые мешки и аналиауют против диctиллиpoвaннoй воды в течение 48 ч. Диализат отдел ют от выпавшего осадка и лйофильно высушивают. Выход лйофильвв высушенного препарата ВtO г, Актйбность полученного препарата 100- ISO ЕЛ.The ba / glast proteins are burnt off by precipitating with ammonium sulphate, From the first extract - at 0, 35 saturation, and from the second - at 0.3 kaschenin, Osaaki is separated by filira tion. A collagenase preparation is precipitated from both filtrates by adding ammonium sulphate to the steppes O, 5-O, 6, After that, the mixture is combined and subjected to settling for 1618 hours, the sludge is siphoned off, and the precipitate is separated by fillet under vacuum. The precipitate containing the enzyme is dissolved in three volumes of water to the mass of the precipitate, poured into plastic bags and analyzed against distilled water for 48 hours. The dialysate is separated from the precipitated precipitate and lyophilized. The output of the lyophilin of the dried preparation BtO g, Actability of the obtained preparation 100- ISO EL.
Йевольэовавне преолагаемого способа ао(9&(1 ет сввзвть стоимость йаготой е и препарата коплагеназн го действи , Сократить производственные площади и объемыEqual to the proposed method of joint venture (9 & 1
реакииоицых смесей при .роизводстве этого препарата, интенсифицировать технологический процесс, а также упростить технологическую схему.reakioitsyh mixtures in the production of this drug, to intensify the process, as well as simplify the technological scheme.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU762400605A SU603389A1 (en) | 1976-08-25 | 1976-08-25 | Method of preparing sunstance of collagenasic action |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU762400605A SU603389A1 (en) | 1976-08-25 | 1976-08-25 | Method of preparing sunstance of collagenasic action |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU603389A1 true SU603389A1 (en) | 1978-04-25 |
Family
ID=20675630
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU762400605A SU603389A1 (en) | 1976-08-25 | 1976-08-25 | Method of preparing sunstance of collagenasic action |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU603389A1 (en) |
-
1976
- 1976-08-25 SU SU762400605A patent/SU603389A1/en active
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