SU452582A1 - Novobiocin deposition method - Google Patents
Novobiocin deposition methodInfo
- Publication number
- SU452582A1 SU452582A1 SU1855302A SU1855302A SU452582A1 SU 452582 A1 SU452582 A1 SU 452582A1 SU 1855302 A SU1855302 A SU 1855302A SU 1855302 A SU1855302 A SU 1855302A SU 452582 A1 SU452582 A1 SU 452582A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- novobiocin
- deposition method
- precipitation
- purity
- activity
- Prior art date
Links
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- Saccharide Compounds (AREA)
Description
1one
Изобретение относитс к медицине.This invention relates to medicine.
Известен способ очистки новобиоцина методом осаждени целевого продукта из спиртового раствора водой. Полученный препарат обладает активностью 600 ед/мг и дл повышени чистоты продукта его подвергают дальнейшей перекристаллизации.A known method of cleaning novobiocin by precipitating the target product from an alcohol solution with water. The resulting preparation has an activity of 600 units / mg and is subjected to further recrystallization to increase the purity of the product.
Дл повышени чистоты препарата и сокращени длительности процесса предлагаетс осаждение осуш,ествл ть водным ацетоном .In order to increase the purity of the preparation and reduce the duration of the process, it is proposed to dry the precipitate using aqueous acetone.
Предлагаемый способ осаждени новобиоцина осуществл ют следующим образом. Спиртовой элюат новобиоципа с активностью не выше 30.000 ед/мл смешивают с одним объемом ацетона и двум объемами подогретой доThe proposed method of precipitation of novobiocin is carried out as follows. Novobiocip alcohol eluate with activity not higher than 30.000 u / ml is mixed with one volume of acetone and two volumes heated to
38-40°С дистиллированной воды. Смесь подкисл ют 2 N раствором сол ной кислоты до рН 2,0-2,5. Осаждение ведут в течение 24 час при комнатной температуре. Полученный осадок новобиоцина-кислоты отфильтровывают. Маточник с концентрацией 550-770 ед/мл возвращают на сорбцию. Выход на стадии осаждени составл ет 75-78%. Активность новобиоцина-кислоты 820-920 ед/мг.38-40 ° C distilled water. The mixture is acidified with a 2 N hydrochloric acid solution to a pH of 2.0-2.5. The precipitation is carried out for 24 hours at room temperature. The resulting precipitate of novobiocin acid is filtered off. The mother liquor with a concentration of 550-770 units / ml return to sorption. The yield at the precipitation stage is 75-78%. The activity of novobiocin-acid 820-920 u / mg.
Предмет изобретени Subject invention
Способ осаждени новобиоцина из спиртовых элюатов, отличающийс тем, что, с целью повышени чистоты препарата и сокращени длительности процесса, осаждение осуществл ют водным ацетоном.A method of precipitating novobiocin from alcoholic eluates, characterized in that, in order to increase the purity of the preparation and shorten the duration of the process, the precipitation is carried out with aqueous acetone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1855302A SU452582A1 (en) | 1972-12-07 | 1972-12-07 | Novobiocin deposition method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1855302A SU452582A1 (en) | 1972-12-07 | 1972-12-07 | Novobiocin deposition method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU452582A1 true SU452582A1 (en) | 1974-12-05 |
Family
ID=20534552
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU1855302A SU452582A1 (en) | 1972-12-07 | 1972-12-07 | Novobiocin deposition method |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU452582A1 (en) |
-
1972
- 1972-12-07 SU SU1855302A patent/SU452582A1/en active
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