SU340693A1 - METHOD FOR PRODUCING ENZYME PREPARATION - Google Patents
METHOD FOR PRODUCING ENZYME PREPARATIONInfo
- Publication number
- SU340693A1 SU340693A1 SU1360608A SU1360608A SU340693A1 SU 340693 A1 SU340693 A1 SU 340693A1 SU 1360608 A SU1360608 A SU 1360608A SU 1360608 A SU1360608 A SU 1360608A SU 340693 A1 SU340693 A1 SU 340693A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- enzyme preparation
- producing enzyme
- calcium chloride
- drying
- culture medium
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 title claims description 9
- 108090000790 Enzymes Proteins 0.000 title claims description 9
- 238000002360 preparation method Methods 0.000 title claims description 4
- 238000004519 manufacturing process Methods 0.000 title claims description 3
- 238000001035 drying Methods 0.000 claims description 5
- 241000194103 Bacillus pumilus Species 0.000 claims description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L cacl2 Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 239000001963 growth media Substances 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 229910052751 metal Inorganic materials 0.000 claims description 2
- 239000002184 metal Substances 0.000 claims description 2
- 150000002739 metals Chemical class 0.000 claims description 2
- 230000001376 precipitating Effects 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- 239000003381 stabilizer Substances 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims 1
- 229940088598 Enzyme Drugs 0.000 description 4
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drugs Drugs 0.000 description 2
- 230000002255 enzymatic Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 229940079919 digestives Enzyme preparations Drugs 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- -1 for example Inorganic materials 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
Description
Изобретение относитс к области микробиологии .This invention relates to the field of microbiology.
Известен способ получени ферментных препаратов путем выраш,ивани их продуцента , например Bacillus mesentericus, отделени клеток от культуральной жидкости, концентрировани культуральной среды, осаждени ферментов с последующей их сушкой .A method of producing enzyme preparations is known by growing, wiping them, for example, Bacillus mesentericus, separating the cells from the culture liquid, concentrating the culture medium, precipitating the enzymes and then drying them.
Известный снособ не дает высокого выхода препарата, а при сушке наблюдаютс значительные потери ферментативной активности .The known method does not give a high yield of the drug, and during drying there is a significant loss of enzymatic activity.
Цель изобретени - увеличение выхода и иолучен-ие ирепарата высокой активности.The purpose of the invention is to increase the yield and resultant high-activity drug.
Дл этого после осаждени осадок раствор ют в 1воде и добавл ют стабилизатор - соли двухвалентных металлов, например, MnS04, ZnS04, в количестве 0,001 - 1%.For this, after precipitation, the precipitate is dissolved in water and the stabilizer is added — salts of divalent metals, for example, MnS04, ZnS04, in an amount of 0.001 - 1%.
Ферментный осадок раствор ют в воде до содержани сухих веществ не более 10%, стабилизируют , стандартизируют до заданной активности и полученный раствор высушивают на распылительной сушилке с улавливанием спирта.The enzyme residue is dissolved in water to a solids content of not more than 10%, stabilized, standardized to a given activity, and the resulting solution is dried on a spray dryer with alcohol trapping.
денным от многих сопутствующих нримесей, стабилизирующих и предохран ющих его от тепловой инакт-ивации. Иоэтому очии1енный фермент быстрее реагирует на воздействие высоких температур и тер ет свою активность .data from many associated substances that stabilize and protect it from thermal inactivation. Therefore, this enzyme reacts faster to high temperatures and loses its activity.
Дл предотвращени потери ферментативной активности в раствор фермента вводитс хлористый кальций (0,2% к объему BbicyuJHваемого раствора).To prevent the loss of enzymatic activity, calcium chloride is injected into the enzyme solution (0.2% by volume of the Bbicyu solution).
Были проведеиы опыты с амилазоГ), нротеазой и молокосвертывающим фермеитом, в растворы которых вводилс хлористый кальций .Experiments were carried out with amylazoid, nrotease, and milk-clotting fermitite, in which calcium chloride was injected.
Полученные данные приведены в таблице.The data obtained are shown in the table.
Выход ферментов, %The output of enzymes,%
Сушку 1 т стабилизированного препарата по данному способу можно осуществить за 12 час на распылительной сушилке производительностью 500 кг нспарениой влаги в 1 час.Drying 1 ton of the stabilized preparation according to this method can be carried out in 12 hours on a spray dryer with a capacity of 500 kg with evaporation of moisture per hour.
Технологическа схема производства очипаенного ферментного пренарата мезентерина (ГК) с использованием этого способа сушки приобретает следующий вид:The technological scheme of the production of the purified enzyme prenarata mesenterin (GC) using this method of drying takes the following form:
ферментаци , -фильтрацн , вакуум-концентрирование , осаждение, растворение осадка+ стабилизаци + стандартизаци , сушка распылением и упаковка.fermentation, filtration, vacuum concentration, precipitation, precipitate dissolution + stabilization + standardization, spray drying and packaging.
Способ был испытан на культуре Bacillus mesentericus как в лаборатории, так и в полузаводских и заводских услови х.The method was tested in Bacillus mesentericus culture both in the laboratory and in semi-plant and plant conditions.
Предмет изобретени Subject invention
Claims (2)
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU904813592A Addition SU1724039A2 (en) | 1990-04-13 | 1990-04-13 | Electrothermal soil disinfecting agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU340693A1 true SU340693A1 (en) |
Family
ID=
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5217890A (en) * | 1991-08-20 | 1993-06-08 | Eastman Kodak Company | Stabilized composition containing creatine kinase and protein having blocked sulfhydryl groups |
| US5468622A (en) * | 1990-04-03 | 1995-11-21 | Immunomatrix, Inc. | Salt stabilization of antibody-enzyme conjugates heat-dried into paper |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5468622A (en) * | 1990-04-03 | 1995-11-21 | Immunomatrix, Inc. | Salt stabilization of antibody-enzyme conjugates heat-dried into paper |
| US5637468A (en) * | 1990-04-03 | 1997-06-10 | Immunomatrix, Inc. | Salt stabilization of antibody-enzyme conjugates heat-dried into paper |
| US5217890A (en) * | 1991-08-20 | 1993-06-08 | Eastman Kodak Company | Stabilized composition containing creatine kinase and protein having blocked sulfhydryl groups |
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