SU333948A1 - METHOD FOR PREPARATION OF ENZYME SOLUTIONS - Google Patents
METHOD FOR PREPARATION OF ENZYME SOLUTIONSInfo
- Publication number
- SU333948A1 SU333948A1 SU1469480A SU1469480A SU333948A1 SU 333948 A1 SU333948 A1 SU 333948A1 SU 1469480 A SU1469480 A SU 1469480A SU 1469480 A SU1469480 A SU 1469480A SU 333948 A1 SU333948 A1 SU 333948A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- solution
- preparation
- enzyme
- enzyme solutions
- enzymes
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 title description 12
- 108090000790 Enzymes Proteins 0.000 title description 12
- 239000000243 solution Substances 0.000 description 11
- 229940088598 Enzyme Drugs 0.000 description 6
- 229940110715 ENZYMES FOR TREATMENT OF WOUNDS AND ULCERS Drugs 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 5
- 229940020899 hematological Enzymes Drugs 0.000 description 5
- 102000004142 Trypsin Human genes 0.000 description 3
- 108090000631 Trypsin Proteins 0.000 description 3
- 239000012266 salt solution Substances 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 229960001322 trypsin Drugs 0.000 description 3
- 239000012588 trypsin Substances 0.000 description 3
- 230000000875 corresponding Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000011330 Armoracia rusticana Nutrition 0.000 description 1
- 240000003291 Armoracia rusticana Species 0.000 description 1
- 229940072417 Peroxidase Drugs 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 108090000437 Peroxidases Proteins 0.000 description 1
- 230000024881 catalytic activity Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Description
Изобретение относитс к медицине и биологии .The invention relates to medicine and biology.
Известен способ приготовлени растворов ферментов путем растворени ферментов в водно-солевом растворе.A known method of preparing solutions of enzymes by dissolving the enzymes in a water-salt solution.
Цель изобретени - повышение устойчивости растворов ферментов при хранении. Это достигаетс тем, что ферменты, например трипсин , предварительно раствор ют в безводном диметилсульфоксиде с последующим введением полученного раствора в водно-солевую среду перед использованием.The purpose of the invention is to increase the stability of enzyme solutions during storage. This is achieved by the fact that enzymes, such as trypsin, are previously dissolved in anhydrous dimethyl sulfoxide, followed by introduction of the resulting solution into the water-salt medium before use.
Пример 1. Навеску а - химотрипсина (до 60 мг мл) раствор ют в безводном диметилсульфоксиде . Этот раствор может хранитьс в течение продолжительного времени при комнатной температуре. Дл получени раствора активного фермента определенный объем органического раствора ввод т в 20-кратный избыток водного солевого раствора при рН 3,0-4,8. Через 40 мин фермент полностью восстанавливает активность.Example 1. A portion of a-chymotrypsin (up to 60 mg ml) is dissolved in anhydrous dimethyl sulfoxide. This solution can be stored for a long time at room temperature. To obtain a solution of the active enzyme, a certain volume of an organic solution is introduced into a 20-fold excess of an aqueous salt solution at a pH of 3.0-4.8. After 40 min, the enzyme completely restores activity.
Пример 2. 10 мг трипсина раствор ют в 5 мл безводного диметилсульфоксида. Дл получени активного раствора фермента органический раствор при слабом перемешивании ввод т в соответствующий солевой раствор в соотнощении 1:100. Через 50-60 мин активность фермента достигает 80-85% от максимальной .Example 2 10 mg trypsin is dissolved in 5 ml of anhydrous dimethyl sulfoxide. In order to obtain the active solution of the enzyme, the organic solution is introduced into the corresponding saline solution at a ratio of 1: 100 with weak stirring. After 50-60 minutes, the enzyme activity reaches 80-85% of the maximum.
П р и М е р 3. 50 мг пероксидазы из корневищ хрена раствор ют в 1 лгл безводного диметнлсульфоксида . При 100-кратном разведенни этого раствора в соответствующем солевом растворе через 35-40 мин фермент достигает 95% от максимальной активности.PR and MER 3. 50 mg of peroxidase from horseradish rhizomes are dissolved in 1 l of anhydrous dimethyl sulfoxide. At 100-fold dilution of this solution in the corresponding salt solution after 35-40 minutes, the enzyme reaches 95% of the maximum activity.
Предмет изобретени Subject invention
Способ приготовлени растворов ферментов путем растворени ферментов в водно-солевой среде, отличающийс тем, что, с целью повышени нх устойчивости при хранении, ферменты , например трипсин, предварительно раствор ют в безводном диметилсульфоксиде.A method of preparing enzyme solutions by dissolving enzymes in a water-salt medium, characterized in that, in order to increase their storage stability, enzymes, such as trypsin, are previously dissolved in anhydrous dimethyl sulfoxide.
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU333948A1 true SU333948A1 (en) |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6117433A (en) * | 1996-01-31 | 2000-09-12 | Dsm N.V. | Use of compositions comprising stabilized biologically effective compounds |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6117433A (en) * | 1996-01-31 | 2000-09-12 | Dsm N.V. | Use of compositions comprising stabilized biologically effective compounds |
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