SU24082A1 - The method of preparation of biological products - Google Patents

Description

The inventor is the author of the study on natural cellular dyes, according to which the dissemination products of the organism are natural and unavoidable aids for those cellular tissues from which they were formed.

According to the inventor, these natural cellular dyes, which are formed in the body as a result of vital activity and cell destruction, are not only waste products of the body, that is, i.e. pests but also useful and even necessary irritant substances of the respective tissues; their biochemical effects serve as an incentive for cell reproduction and activity.

Biological preparations are saline solutions of decay products obtained from tissues or organs of young healthy animals by autolysis or artificial enzymatic cleavage with a special physicochemical treatment.

For the preparation of biological preparations according to the invention, a known organ is taken, for example a testicle, or a specific tissue, for example a muscle, from a freshly killed animal, carefully cut off with clean, cold water, crushed in a cutlet machine and hung up. The resulting mica is divided in half and each part is separately placed in a corresponding thick-walled glass jar with a narrow neck, one of which is filled with liquid A, and another liquid B. Three times the weight is taken into double, triple or tenth, depending on character fabric. Vessels should be of a definite capacity so that the poured liquid reaches the neck itself. The latter closes tightly with a cork stopper so that there is no free air in the can. Liquid A consists of a solution of hydrochloric acid and chloroform to saturation, and liquid B consists of a solution of soda and chloroform to saturation.

Next, the vessels are placed in a thermostat at a temperature of 35-40, at which autolysis occurs in one flask during an acid reaction, and in the other when alkaline.

By subjecting both autolysates to filtration through several layers of gauze, boiled and dried, both filtrates are poured into a saucepan, in which, after mixing, is set, 70. The liquid is slightly heated to remove chloroform.

Preparations intended for per OS are preserved with ethyl

alcohol and injections are preserved by the addition of phenol

TO V20 / 0.j

It is possible to prepare biologics and;. Otherwise. The product obtained by the aforementioned method is placed in a flask and filled in; it is kept under the same conditions, as described, with a mixture of hydrochloric acid P / o dry pepsin and chloroform to saturation.

After being placed in a thermostat, enzymatic digestion is performed in the acidic j reaction. Next come on the previous Shem. Finally, biopreparations can be prepared, if the appropriate tissue or organ is washed, clean with cold water, crushed, cut in m, weighed and placed in a flask with four times the amount of distilled water, leaving for 1-2 days in the refrigerator (). From time to time the flask is embedded - it is lost.

Next, the autoclave is placed at 5-10; : -1in at 120, after which the contents of the i flask are poured into hot air through | .marly in the pot. The M is pressed and the liquid obtained through the same gauze is drained into the same pan, at which this operation is repeated several times. Then the preparation is left for a day in the cold and the resulting precipitate is filtered off in a cold form, sterilized at 100 for an hour.

The resulting product is added to the lysates prepared as described above. If the infusion is added to a neutral preparation, a neutral reaction is preliminarily established by adding a saturated solution of INSO3 and then the preparation is carried out in the usual order: canning and pouring. In bottles.

The subject matter of the invention.

Claims (3)

1. A method of preparing biologics, characterized in that, in order to obtain salt solutions of the decay products of tissues or organs of young animals by autolysis or artificial enzyme cleavage, the organ (for example, testicle) or tissue (for example, muscles) is washed from the killed animal, crushed and the obtained msu is divided into two equal parts, from which one part is poured with 2% hydrochloric acid solution and chloroform to saturation, the other i / 2 / o soda solution and chloroform to saturation, after which both parts are placed in closed vessels separatelyin a thermostat at a temperature of 35-40, the autolysates are filtered, both filtrates are drained together and, on setting .07, the liquid is heated to remove chloroform. 2. Modification of the method according to claim I, characterized in that the obtained mous is treated with a V-% solution of hydrochloric acid with 1% dry pepsin and chloroform to saturation in order to obtain enzymatic cleavage in an acidic medium. 3. Modification of the method according to claim 1, which is different in that diluted mash with water after leaving it at a temperature of 1-2 days is placed in an autoclave for 5-10 minutes at 120, after filtering in a hot state, the liquid obtained by pressing meat , left in the cold, the residue is filtered off and sterilized at 100, finally the obtained product is added to the lysates, extracted according to the method indicated in items 1 and 2.