SU1757537A1 - Microalgae growing tank - Google Patents

Abstract

Usage: industrial cultivation of microalgae as a source of feed protein and food additives. SUMMARY OF THE INVENTION: The installation comprises an open container with an internal coating, a vertical longitudinal partition placed in the container with the formation of a closed channel for the circulation of medium vr, an agent for the consumption of microalgae suspension. The side ends of the partition are made extended with a smooth contour. Shafts with brushes are located on the sides of the partition for cleaning the walls and additional mixing, equipped with a reversible drive. The installation is equipped with a medium discharge system consisting of three vertical pipes placed in a tank, the inlet sections of which are arranged stepwise at three levels to select the medium in different stages of the synchronous growth of microalgae. The inner coating of the container is made of glass. 2 hp f-ly, Zil.

Description

The invention relates to devices for the industrial cultivation of microalgae and can be used for the production of feed protein-vitamin supplements, foodstuffs, medicinal, perfumery and other preparations.

Known shallow (8-12 cm) installation for the cultivation of microalgae (A.M. Muzafarov, T.T. Taubaev Chlorella, published by FAN, Tashkent, 1974, pp. 37-38).

The disadvantage of these installations is the complexity of the design, high energy consumption, increased consumption of C02, difficult sanitary processing and reduced productivity due to a number of factors.

Closest to the proposed installation to the technical essence is the 4-coal wood lotkovoy installation

(V.V. Pinevich et al. Typical plant for the mass cultivation of single-celled algae. Plant physiologists. TN bailout. 6,1964, p. 1084-1088, fig. I).

The disadvantage of the installation is that a wooden board is used for its construction. Inside the installation is lined with a low-strength plastic wrap, resulting in difficult sanitary treatment. The absence in the installation of mechanisms for the effective mixing of suspensions, insufficient aeration and illumination leads to a decrease in the intensity of photosynthesis and an increase in physiological inactive cells. As a result, the cells settle down on the internal surface of the plant, they accumulate on the bottom, decompose. they are a source of contamination with organic matter. When ripe

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It is impossible to ensure synchronism of cell culture development by culture (6-8 days) and constant suspension of the suspension in the plant, for 30-60 days.

The use of the finished 5% carbon dioxide leads to a significant consumption, requires the use of a compressor and is associated with a high cost of electricity.

In order to improve performance, quality of the final product and reduce gas consumption, a block tray installation is proposed, characterized in that its internal surface to eliminate algae cell overgrowth, improve the efficiency of use of light energy and the convenience of preventive treatment is lined with arbitrary shaped glass plates. contributing to the effective saturation of the suspension with cheap atmospheric carbon dioxide and oxygen, which are necessary for photosynthesis algae, at the same time also contributing to the acceleration of laminar and turbulent flow of the suspension; applied a mobile device with paddle wheels, vertical shafts with brushes, a cable, designed to raise sedentary cells and provide additional efficient mixing of the suspension; drain pipes were installed for layering of multi-age synchronous cells of the suspension.

Typical reinforced concrete trays with radially rounded end walls, which are separated by 0.6 m from the partition wall with rounded ends, 2 m wide, 30 m long, 80 cm high, can be used for the construction of the reactor. The inner surface of the trays is lined with arbitrary glass tiles form, you can use broken glass.

Figure 1 shows a tray installation, top view; figure 2 - installation in cross section, side view; Fig. 3 shows a pipe system for layer-by-layer separation of synchronous cells and cladding glass plates, side view.

The plant for the cultivation of microalgae consists of a housing 1, a dividing partition 2 with rounded ends 3, an electric pump 4 with impeller 5, suction b and discharge 7 pipes, an injector 8, a rotameter 9, a valve-valve of the rotameter 10, a frame 11c wheels 12, paddle agitators 13 with brushes 14 on vertical shafts 15, belt 16, reversing electric motor 17, gearbox 18, pulleys 19, drums 20 for winding cable tape 21, drain pipe system consisting of upper tube 22, middle 23 and lower 24. fitted with a crane 25 and facing with ekl nnyh plates 26.

The installation works as follows. Drinking-quality tap water is poured into the cleaned and disinfected unit at a level of 24 cm.

0 volume (15 m3) with a temperature in the range. Cultivation is carried out in natural light (about 8-75 thousand lk). The suspension is stirred during the daytime, starting at 8 o'clock.

5 am to 8 pm Electric pumps operate in automatic mode (20 minutes in the working and 40 minutes in the non-working). A typical nutrient medium (04) is added to water based on 1 liter of water, ammonium sulfate - 0.2 g; calcium phosphate monosubstituted - 0.03 g; calcium sulfate - 0.03 g; sodium bicarbonate - 0.1 g; magnesium sulfate - 0.08 g; potassium chloride - 0.025 g; ferric chloride, 1%

5 solution - 0.15 ml; soil extract - 0.5 ml. Soil extract is prepared from garden soil: 1 gr. Soils are poured over with 3 ml of distilled water (1: 3), shaken for 5 minutes and passed through a filter and added to the medium after filtration. Salts of trace elements are added to the nutrient medium. In the prepared nutrient medium, the initial synchronous culture of microbial growth is taken, taken from the active unit in an amount that provides the initial density, for example, 3-8 mpn. cells per 1 ml of suspension. After that, the pump 4 is turned on, which creates a directional

0 ring slurry flow. The suspension through the pipe 6 is sucked into the pump, where it is picked up by the impeller 5 through the discharge pipe 7 at a speed of 0.5-0.6 m / s is fed to the injector 8, which is attached

5 at the end of the discharge pipe 7.

Due to a strong jet of suspension under a pressure of 1 atm, a reduced pressure area is created through the nozzle of the injector 8, where, as a result of the vacuum, atmospheric air is intensively sucked through the rotameter 9. An air-liquid mixture is formed, which under pressure 0 is ejected into the reactor. , 7-0.8 m / s. Degree of supply

5 suspension by atmospheric air depends on the speed of the outgoing suspension from the injector nozzle 8 on the diameter of the air inlet of the valve-valve 10 of the rotameter. For example, with a nozzle opening diameter of -14 mm, using a pump (ЗК-9) - 45 m3 / h in

Depending on the air gap of the valve valve of the injector, the air supply to the suspension, according to the indication of the rotameter, reaches from 30 m3 / hour to 50 m3 / hour.

After injection, the suspension, acquiring additional turbulent motion, flows through the tray and is actively stirred. Moving along the tray, the flow of the suspension enters the zone of the suction pipe 6 of the pump 4, the cycle of operation is repeated.

Simultaneously with the disconnection of the electropump of the reactor, which mixes the suspension, the reversing electric motor 16 with the blades 13 is automatically switched on, with the brushes 14 on the vertical shafts 15,

The cleaning device operates as follows: after the automatic switching of the reversing electric motor 17, for example, the right rotation of the motor shaft and the gearbox 18, the winding drum 20 begins to rotate slowly and winds the cable 21 of the right impeller on the vertical shaft 15, driving it simultaneously in a slow rotation and translational movement of the frame 11 on the wheels 12. As a result, the brushes 14 intensively remove the settled cells from the inner surface of the trays lined with glass plates 26 and at the same time actively mixed by blades 13 all the thickness of the suspension. The winding drum of the left paddle wheel at this time unwinds the cable. Moving in the right direction to the end edge of the partition 2 in the device, the automatic stop of the reversing electric motor 17 is activated. After it stops, the electric drive automatically turns on in the opposite direction. So the device works for 40 minutes. After automatically for 20 minutes, the motor 4 is turned on to mix the slurry and supply air and CO.

Subsequently, as they mature, i.e. the emergence of cultures on a plateau, on days 3–4 in the reactors, by settling at night time for 10–12 hours, cell synchronization takes place, based on natural sedimentation.

In each reactor, there is a pipe system for layering the discharge of synchronous, same-age physiological active cells. To separate and transfer synchronous cell cultures to other reactors for further cultivation, first open the valve 25 of the drain pipe 22 to drain the upper translucent layer of the daughter cells of the cells, which is fed into

another reactor for further cultivation; then the valve of the drain pipe 23 is opened to drain the middle light green layer of semi-mature cells. The dark green bottom layer of the suspension — mature mother cells — partially remains in the reactor. Subsequently, adding water and nutrient medium to the reactors continues to grow algae.

The use of the proposed plant for the cultivation of microalgae contributes to an increase in productivity by accelerating the yield on the plateau by a factor of 2 to obtain a continuous synchronous culture of algae, which solves the problem of providing good food and obtaining high-quality biomass; it gives the opportunity to get rid of accompanying microalgae-growing plants when growing algae, increases the dry mass yield by 4 times, allows the use of C02 from the atmosphere.

Claims (3)

1. Installation for the cultivation of microalgae, containing a horizontal longitudinal open tank with an internal coating, a carbon dioxide supply pipe and a medium draining system, placed in a capacious vertical longitudinal partition with the formation of a closed channel for circulation of the medium between it and the vessel wall of the tank and the microalgae consumption flow generator , characterized in that, in order to increase the quality of the final product and to reduce gas consumption, the lateral ends of the partition are made In the tank, on the sides of the partition, vertical eaIs are placed with BT1, a wall for cleaning walls and additional mixing, equipped with a reversible drive and mounted for movement along the tank. At this carbon dioxide supply pipe, the ejector is supplied at the outlet, and the system The medium discharge consists of three tubes, the inlet sections of which are placed vertically in the tank and their ends are arranged stepwise at three levels to select the medium at various stages of synchronous growth of microalga s. 2. Installation pop. 1, characterized in that the inner coating of the container is made of glass. 3. The installation according to claim 1, in connection with the fact that the carbon dioxide supply pipe is in communication with the atmosphere. Air g 1 K 13 15 16 gshgl i5 Schig.1 10, 1 I 74 vTXN1 FIG 2 1PG y y ZB 25 NX / XSV