CN113214352B - Novel device and method for extracting protein from microalgae - Google Patents

Novel device and method for extracting protein from microalgae Download PDF

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Publication number
CN113214352B
CN113214352B CN202110579253.9A CN202110579253A CN113214352B CN 113214352 B CN113214352 B CN 113214352B CN 202110579253 A CN202110579253 A CN 202110579253A CN 113214352 B CN113214352 B CN 113214352B
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cylinder
block
fixed
reaction
electric push
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CN113214352A (en
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马静
徐田朋
曹君旸
韩照莹
徐军田
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Jiangsu Ocean University
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Jiangsu Ocean University
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/34Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/145Extraction; Separation; Purification by extraction or solubilisation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

The invention discloses a novel device for extracting protein from microalgae, which belongs to the technical field of microalgae protein extraction and comprises a bottom box, wherein a conical block is fixed on the inner side of the bottom box, a filtering frame is fixed on the upper end of the conical block, an adjusting electric push rod is embedded and installed in the middle of the conical block, a blocking block is fixed at the movable end of the adjusting electric push rod, lifting electric push rods are symmetrically installed on the outer side of the bottom box, a cylinder is arranged on the inner side of the bottom box, a connecting block is symmetrically fixed on the outer side of the cylinder, the connecting block is fixedly connected with the lifting electric push rods, a through hole is formed in a bottom plate of the cylinder, an installation block is symmetrically fixed on the inner wall of the cylinder, a slot is formed in the installation block, and a reaction container component is arranged on the inner side of the cylinder; the invention is provided with the filter frame which can filter finished liquid, and the position of the cylinder is moved so as to clean the filter frame and impurities in the filter frame, and the reaction cylinder can be taken out so as to clean the interior of the reaction cylinder conveniently, thereby improving the cleanliness.

Description

Novel device and method for extracting protein from microalgae
Technical Field
The invention relates to the technical field of microalgae protein extraction, in particular to a novel device and a method for extracting protein from microalgae.
Background
Microalgae are micro-algae populations whose morphology is distinguishable under a microscope, and generally refer to a general term for microorganisms containing chlorophyll a and capable of photosynthesis, which belongs to one type of protozoa. Microalgae mass-cultured or produced by biotechnology belong to four phylum: cyanobacteria, chlorophyta, chrysophyta, and Rhodophyta. In the process of culturing microalgae, the protein in the microalgae needs to be extracted, and an enzymolysis device is needed in the extraction process.
The patent specification of patent publication No. CN211713107U discloses an enzymolysis device for extracting protein from microalgae, which comprises a hollow barrel, one side of cavity barrel bottom is fixedly installed with the support frame, and the top fixedly connected with cap of cavity barrel, the inlet pipe has been cup jointed to one side of cap top fixedly, and the one end of inlet pipe extends to the inner chamber of cavity barrel, the grease extraction pipe has been cup jointed to the opposite side of cap top fixedly, the drain chute has been seted up to the bottom of cavity barrel, and the inner chamber fixed mounting of drain chute has the filter. The enzymolysis device for extracting protein from microalgae is convenient for better enzyme-inactivating treatment of the solution in the reaction vessel by the mutual coordination among the heating vessel, the heater, the control line and the controller.
The scheme still has the defects that: the reaction vessel in above-mentioned scheme is after using, is inconvenient to clear up its inside, and above-mentioned scheme is provided with the filter and can filters the discharge material, but the clearance is inconvenient after the filter uses.
Therefore, there is a need to design a novel device and method for extracting protein from microalgae.
Disclosure of Invention
The invention aims to provide a novel device and a method for extracting protein from microalgae, which are used for solving the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a novel draw protein from microalgae device, includes the base tank, the outside symmetry of base tank is provided with the fluid-discharge tube that has the valve, the inboard of base tank is fixed with the toper piece, the upper end of toper piece is fixed with the filter frame, the middle part embedding of toper piece is installed and is adjusted electric putter, the expansion end of adjusting electric putter is fixed with the sprue, the outside symmetry of base tank is installed and is gone up and down electric putter, the inboard of base tank is provided with the drum, the outside symmetry of drum is fixed with the connecting block, connecting block and lift electric putter fixed connection, the through-hole has been seted up on the bottom plate of drum, the inner wall symmetry of drum is fixed with the installation piece, the slot has been seted up on the installation piece, the inboard of drum is provided with the reaction vessel subassembly, the upper end of drum is provided with the apron.
Further, in the novel device for extracting protein from microalgae, the reaction container assembly comprises a reaction cylinder, a liquid outlet pipe, rectangular blocks, handshakes and inserting blocks, wherein the liquid outlet pipe is connected to the bottom end of the reaction cylinder, the liquid outlet pipe is connected with the blocking blocks in a clamping mode, the rectangular blocks are symmetrically fixed on the outer side of the reaction cylinder, the handshakes are fixed at the upper ends of the rectangular blocks, and the inserting blocks are fixed at the lower ends of the rectangular blocks and are connected with the inserting grooves in a clamping mode.
Furthermore, in the novel device for extracting protein from microalgae, a rubber layer is arranged on the outer side of the blocking block.
Further, in the novel device for extracting protein from microalgae, hot water is arranged inside the cylinder.
Furthermore, in the novel device for extracting protein from microalgae, the cover plate is in threaded connection with the cylinder.
Furthermore, in the novel device for extracting protein from microalgae, the top of the blocking block is conical.
Further, in the novel device for extracting proteins from microalgae, the cylinder is provided with a heater.
Furthermore, in the novel device for extracting protein from microalgae, the upper end of the cover plate is fixed with the handle.
Further, in the novel device for extracting protein from microalgae, the bottom end of the cylinder is contacted with the upper end of the filter frame.
The application method of the novel device for extracting protein from microalgae comprises the following steps:
s1: taking down the cover plate, adding a reaction solution into the reaction cylinder, and covering the cover plate;
s2: after the reaction is finished, the electric push rod is started to be regulated, the blocking block is driven to move, and the blocking block moves out of the liquid outlet pipe and the through hole, namely, finished liquid in the reaction cylinder can be discharged;
s3: after the finished liquid is discharged, the control block is moved back to the original position, the finished liquid is filtered by the filtering frame, and the liquid discharge pipe is opened to discharge the finished liquid;
s4: the cover plate is taken down, the handles on the two sides are manually held, the plug block is separated from the slot, and the liquid outlet pipe is separated from the plug block, so that the reaction cylinder can be taken out, and the interior of the reaction cylinder is conveniently cleaned;
s5: and starting the lifting electric push rod to lift the position of the cylinder so as to clean the filtering frame and impurities in the filtering frame.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention can discharge the finished liquid into the bottom box, and the filter frame is arranged to filter the finished liquid, and the position of the cylinder is moved to clean the filter frame and impurities in the filter frame.
2. The invention can take out the reaction cylinder, is convenient for cleaning the inside of the reaction cylinder, improves the cleanliness and avoids pollution caused by residual liquid.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is an enlarged schematic view of the structure of FIG. 1A according to the present invention;
FIG. 3 is an enlarged schematic view of the structure of FIG. 1B according to the present invention;
FIG. 4 is a front view of the present invention;
FIG. 5 is a schematic view of the cylinder of the present invention in a position raised;
in the drawings, the list of components represented by the various numbers is as follows:
the device comprises a 1-bottom box, a 2-conical block, a 3-liquid discharge pipe, a 4-filter frame, a 5-adjusting electric push rod, a 6-blocking block, a 7-lifting electric push rod, an 8-connecting block, a 9-reaction container assembly, a 91-reaction cylinder, a 92-liquid outlet pipe, a 93-rectangular block, a 94-handshake, a 95-inserting block, a 10-installing block, an 11-inserting groove, a 12-cover plate, a 13-handle, a 14-cylinder and a 15-through hole.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-5, the present invention provides a technical solution: the utility model provides a novel draw protein from little algae device, including the base case 1, the upper portion of base case 1 sets up to the opening form, and the outside symmetry of base case 1 is provided with the fluid-discharge tube 3 that has the valve, and the inboard of base case 1 is fixed with toper piece 2, and the upper end of toper piece 2 is fixed with filter frame 4, and filter frame 4 can filter finished product liquid, and electric putter 5 is installed in the middle part embedding of toper piece 2, and electric putter 5 is adjusted's movable end is fixed with the locating piece 6, and the top of locating piece 6 sets up to the coniform, and the outside of locating piece 6 is provided with the rubber layer.
The outside symmetry of base box 1 installs lift electric putter 7, the inboard of base box 1 is provided with drum 14, install the heater on the drum 14, the inside of drum 14 is provided with hot water, the outside symmetry of drum 14 is fixed with connecting block 8, connecting block 8 and lift electric putter 7 fixed connection, through-hole 15 has been seted up on the bottom plate of drum 14, through-hole 15 and the joint of shutoff piece 6, the inner wall symmetry of drum 14 is fixed with installation piece 10, slot 11 has been seted up on the installation piece 10, the inboard of drum 14 is provided with reaction vessel assembly 9, the upper end of drum 14 is provided with apron 12, the upper end of apron 12 is fixed with handle 13, apron 12 and drum 14 threaded connection, the inboard of apron 12 is provided with the internal thread, the outside of drum 14 is provided with the external screw thread, the bottom of drum 14 contacts with the upper end of filter frame 4.
Wherein, reaction vessel assembly 9 includes reaction section of thick bamboo 91, drain pipe 92, rectangular block 93, handles 94 and inserted block 95, and the bottom of reaction section of thick bamboo 91 is connected with drain pipe 92, drain pipe 92 and the joint of sprue 6, and the outside symmetry of reaction section of thick bamboo 91 is fixed with rectangular block 93, and rectangular block 93's upper end is fixed with handles 94, and rectangular block 93's lower extreme is fixed with inserted block 95, and inserted block 95 and slot 11 joint.
The application method of the novel device for extracting protein from microalgae comprises the following steps:
s1: removing the cover plate 12, adding a reaction solution into the reaction cylinder 91, and covering the cover plate 12;
s2: after the reaction is completed, the electric push rod 5 is started to be regulated, the blocking block 6 is driven to move, and the blocking block is moved out of the liquid outlet pipe 92 and the through hole 15, namely, finished liquid in the reaction cylinder 91 can be discharged;
s3: after the finished liquid is discharged, the control block 6 moves back to the original position, the finished liquid is filtered by the filter frame 4, and the liquid discharge pipe 3 is opened to discharge the finished liquid;
s4: the cover plate 12 is taken down, the handles 94 on the two sides are manually held, the insert block 95 is separated from the slot 11, the liquid outlet pipe 92 is separated from the blocking block 6, and the reaction cylinder 91 can be taken out, so that the interior of the reaction cylinder is conveniently cleaned, and the pollution of residual liquid to the interior of the reaction cylinder 91 is avoided;
s5: the lifting electric push rod 7 is started, and the position of the cylinder 14 is lifted, so that the filter frame 4 and impurities in the filter frame are cleaned conveniently, and the blocking condition is avoided.
In the description of the present specification, the descriptions of the terms "one embodiment," "example," "specific example," and the like, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended only to assist in the explanation of the invention. The preferred embodiments are not exhaustive or to limit the invention to the precise form disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best understand and utilize the invention. The invention is limited only by the claims and the full scope and equivalents thereof.

Claims (9)

1. The utility model provides a novel draw protein from little algae device, includes base box (1), its characterized in that: the automatic filter is characterized in that drain pipes (3) with valves are symmetrically arranged on the outer side of the bottom box (1), conical blocks (2) are fixed on the inner side of the bottom box (1), a filter frame (4) is fixed on the upper end of each conical block (2), an adjusting electric push rod (5) is embedded and installed in the middle of each conical block (2), a blocking block (6) is fixed on the movable end of each adjusting electric push rod (5), lifting electric push rods (7) are symmetrically installed on the outer side of the bottom box (1), cylinders (14) are arranged on the inner side of the bottom box (1), connecting blocks (8) are symmetrically fixed on the outer side of each cylinder (14), the connecting blocks (8) are fixedly connected with the lifting electric push rods (7), through holes (15) are formed in the bottom plate of each cylinder (14), mounting blocks (10) are symmetrically fixed on the inner wall of each cylinder (14), inserting grooves (11) are formed in the mounting blocks (10), reaction container assemblies (9) are arranged on the inner side of each cylinder (14), and cover plates (12) are arranged on the upper ends of the cylinders (14).
The reaction container assembly (9) comprises a reaction cylinder (91), a liquid outlet pipe (92), rectangular blocks (93), a handshake (94) and an inserting block (95), wherein the liquid outlet pipe (92) is connected to the bottom end of the reaction cylinder (91), the liquid outlet pipe (92) is connected with a blocking block (6) in a clamping mode, the rectangular blocks (93) are symmetrically fixed to the outer side of the reaction cylinder (91), the handshake (94) is fixed to the upper end of each rectangular block (93), the inserting block (95) is fixed to the lower end of each rectangular block (93), and the inserting block (95) is connected with a slot (11) in a clamping mode.
2. The novel device for extracting protein from microalgae according to claim 1, wherein the device is characterized in that: and a rubber layer is arranged on the outer side of the blocking block (6).
3. The novel device for extracting protein from microalgae according to claim 1, wherein the device is characterized in that: the inside of the cylinder (14) is provided with hot water.
4. The novel device for extracting protein from microalgae according to claim 1, wherein the device is characterized in that: the cover plate (12) is in threaded connection with the cylinder (14).
5. The novel device for extracting protein from microalgae according to claim 1, wherein the device is characterized in that: the top of the blocking block (6) is conical.
6. The novel device for extracting protein from microalgae according to claim 1, wherein the device is characterized in that: a heater is mounted on the cylinder (14).
7. The novel device for extracting protein from microalgae according to claim 1, wherein the device is characterized in that: the upper end of the cover plate (12) is fixed with a handle (13).
8. The novel device for extracting protein from microalgae according to claim 1, wherein the device is characterized in that: the bottom end of the cylinder (14) is contacted with the upper end of the filter frame (4).
9. The method of using a novel apparatus for extracting protein from microalgae as claimed in claim 1, comprising the following steps:
s1: removing the cover plate (12), adding a reaction solution into the reaction cylinder (91), and covering the cover plate (12);
s2: after the reaction is finished, the electric push rod (5) is started to be regulated, the blocking block (6) is driven to move, and the blocking block moves out of the liquid outlet pipe (92) and the through hole (15), namely, finished liquid in the reaction cylinder (91) can be discharged;
s3: after the finished liquid is discharged, the control block (6) is moved back to the original position, the finished liquid is filtered by the filter frame (4), and the liquid discharge pipe (3) is opened to discharge the finished liquid;
s4: the cover plate (12) is taken down, the handles (94) on the two sides are manually held, the insert block (95) is separated from the slot (11), the liquid outlet pipe (92) is separated from the blocking block (6), and the reaction cylinder (91) can be taken out, so that the interior of the reaction cylinder is cleaned conveniently;
s5: and starting the lifting electric push rod (7) to lift the position of the cylinder (14) so as to clean the filtering frame (4) and impurities in the filtering frame.
CN202110579253.9A 2021-05-26 2021-05-26 Novel device and method for extracting protein from microalgae Active CN113214352B (en)

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CN113214352B true CN113214352B (en) 2023-07-25

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1757537A1 (en) * 1991-01-16 1992-08-30 Институт Ботаники Ан Казсср Microalgae growing tank
CN1158091A (en) * 1995-07-04 1997-08-27 汉斯·格奥尔格·胡贝尔 Device for removing solids from liquid flowing along a channel
CN107840382A (en) * 2017-10-25 2018-03-27 江阴市弘诺机械设备制造有限公司 A kind of sewage disposal device with cleaning plant
CN211057006U (en) * 2019-11-07 2020-07-21 内蒙古蓝一生物科技有限公司 A draw jar for drawing phycocyanin
CN211713107U (en) * 2019-12-26 2020-10-20 云南爱尔康生物技术有限公司 Enzymolysis device for extracting protein from microalgae

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1757537A1 (en) * 1991-01-16 1992-08-30 Институт Ботаники Ан Казсср Microalgae growing tank
CN1158091A (en) * 1995-07-04 1997-08-27 汉斯·格奥尔格·胡贝尔 Device for removing solids from liquid flowing along a channel
CN107840382A (en) * 2017-10-25 2018-03-27 江阴市弘诺机械设备制造有限公司 A kind of sewage disposal device with cleaning plant
CN211057006U (en) * 2019-11-07 2020-07-21 内蒙古蓝一生物科技有限公司 A draw jar for drawing phycocyanin
CN211713107U (en) * 2019-12-26 2020-10-20 云南爱尔康生物技术有限公司 Enzymolysis device for extracting protein from microalgae

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