SU1677711A1 - Method for simulation of dystrophic varus deformity of the femoral neck - Google Patents

Abstract

The invention relates to medicine and can be applied in modeling dystrophic varus deformity of the femoral neck. The goal is to approach the clinical course due to the complete reproduction of the clin-x-ray picture. In anesthetized animal, the capsule of the hip joint is dissected along the posterior surface of the femoral neck and the posterior retinal retinal vessels of the femoral neck are crossed. Radiographically on the 30th and 90th days, compliance with clinical pathology was found.

Description

The invention relates to medicine and can be applied in experimental modeling of pathological conditions of the proximal end of the femur.

The aim of the invention is to approach the clinical course by fully reproducing the clinical x-ray picture.

The method is carried out as follows

In the area of the left hip joint of the rabbit the wool is cut. The rabbit is fixed in the supine position. The operative field is treated twice with a 1% solution of an alcoholic iodine tincture. Under local anesthesia of tf, with a 25% solution of novocaine, a section, arched around the large trochanter of the thigh, dissected through the skin and revealed a large trochanter of the femur. The scalpel is cut and displaced up to the bone-cartilage plate apophysis of the large den with the average annual muscle attached to it. On the back of the femoral neck cut through the capsule of the hip joint. Then, inserting the tip of the scalpel into the joint's joint, the intersection of the retinuclear vessels along the posterior semicircle of the femoral neck and their hemostasis are performed. After that, the joint capsule is sutured tightly. Apophysis of the greater skewer is fixed to its bed with catgut sutures. The wound is sutured in layers tightly. After 30 and 90 days, an x-ray of the hip joint was performed.

The proximal metaphysis of the femoral bones of animals is subjected to histological examination. To this end, these bone segments are fixed in a 20% neutral formalin solution. Then, preparation, decalcification in a 6% solution of nitric acid, degreasing with alcohol, and pouring into celloidin are carried out sequentially. On the microtome they drop

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Cut in the frontal plane with a thickness of 5–8 µm, followed by hemotoxilin and eosin staining according to the generally accepted Van Gieson technique.

Results of experimental studies.

The observation period is 30 days. The animal moves, slightly limping on the operated limb. The hip is reduced and slightly bent at the hip joint. Passive movements in the hip joint are limited to an angle of 10-15 ° more due to retraction and internal rotation. Radiographically, in the medial parts of the femoral neck, a zone of pathological bone remodeling was determined in the form of marked osteoporosis, dedifferentiation of the beam structure of the cancellous bone. Morphologically, dystrophic changes were observed in the bone tissue of the femoral neck, in its medial regions, where there were areas with bone beams undergoing necrosis and lysis. Such bone beams were deprived of osteocytes. The bone marrow of the femoral neck was replaced with adipose tissue, its myeloid elements were depleted. In some areas in the lower half of the thigh, absorbable bone beams were replaced with fibroreticular tissue. Simultaneously with the resorption processes, reparation processes occur in these areas.

The observation period is 90 days. The animal uses the left lower limb, leaning slightly on it. Radiographically, by this date, cervical deformity developed. At the same time, there was an increase in the size of the greater trochanter of the femur — changes characteristic of the dystrophic vascular deformity of the femoral neck in patients with this disease. False education

joint in the middle third of the femoral neck in experimental animals is characteristic of the stage of varus deformity - coxa vara coll llsls. Morphologically, in all the animals in the femoral neck, bone marrow is replaced with fatty tissue. In the middle third of the neck there were areas with bone beams in the necrosis stage. The latter were deprived of osteocytes. Absorbable bone beams, replaced by fibroreticular tissue, were also found there. Simultaneously with the processes of resorption in these areas there are processes of reparation.

Thus, the proposed method allows to obtain all macroscopic, radiological, histological and clinical signs characteristic of dystrophic varus deformity of the femoral neck - dystrophic coxa vara.

The method of modeling dystrophic varus deformity of the femoral neck is characterized by the following advantages: it allows to reveal a new, previously unknown link in the pathogenesis of the varus deformity of the femoral neck; allows reproducing in the animal experiment a model of the disease.

Claims (1)

Invention Formula A method of modeling dystrophic varus deformity of the femoral neck by creating local ischemia in an experimental animal, characterized in that, in order to approach the clinical course due to the complete reproduction of the clinical x-ray picture, local ischemia is created by occlusion of the posterior retin of the femoral neck vessels.