SU1582129A1 - Method of determining individual sensitivity to treatment in chronic nonspecific disease of the lungs - Google Patents

Abstract

The invention relates to medicine, in particular to laboratory tests in the treatment of pulmonary patients, and can be recommended as a criterion for an early assessment of the effectiveness of therapy. The aim of the invention is to accelerate and improve the accuracy of the method. This goal is achieved by preparing a suspension of washed erythrocytes, mixing the resulting erythrocyte suspension with hydrochloric acid in a volume ratio of 20: 1. Hemolysis is carried out at 37 ° C. The registration of the dynamics of optical density is carried out on a photometric device with an optical logarithm introduced into the electrical circuit. The effectiveness of therapy is judged by the normalization of the profile of the resulting acid hemolysis curves in relation to the average hemolysis curves of healthy individuals.

Description

one

(21) 3930948 / 28-14

(22) J2.07.85

(46) 07/30/90. Bul Number 28

(71) Odessa Medical Institute. N.I.Pirogova

(72) V.M. Yurlov, T. B. Evdokimova and V. P. Kozlov.

(53) 616.07 (088.8)

(56) Prevention — Certain diseases of the internal organs. Ed. Ternovy K.S., Kiev: Vida School, 1983, pp.128-130, 154-157;

(54) METHOD FOR DETERMINING INDIVIDUAL SENSITIVITY TO TREATMENT IN CHRONIC NONSPECIFIC DISEASE OF THE LUNGS

(57) The invention relates to medicine, in particular to laboratory tests in the treatment of pulmonary

patients, and can be recommended as a criterion for an early assessment of the effectiveness of therapy. Purpose invented Shadowing is the acceleration and improved accuracy of the method. This goal is achieved due to the fact that a suspension of washed erythrocytes is prepared, the obtained errocyte suspension is mixed with hydrochloric acid in a volume ratio of 20: 1. Hemolysis is carried out at 37 ° C. The registration of the dynamics of optical density is carried out on a photometric device with an optical logarithm introduced into the electrical circuit. The effectiveness of therapy is judged by the normalization of the profile of the obtained acid hemolysis curves with respect to the average statistical hemolysis curves of healthy individuals.

(I

The invention relates to medicine, in particular, to laboratory research in the treatment of pulmonary patients, and can be recommended as a criterion for an early assessment of the effectiveness of therapy.

The invention is aimed at accelerating and improving the accuracy of the method.

The method is carried out as follows.

A patient is taken 0.2 ml of blood from a finger in 2 ml of isotonic sodium chloride solution. Next, a suspension of washed erythrocytes with an optical density of 0.700 is prepared in the usual manner. The resulting erythrocyte suspension is mixed with 0.02 n. hydrochloric acid in the ratio of 20 + 0.3: 1 + 0.3.

Hemolysis is carried out in a temperature-controlled cuvette at 36.6-37 ° C. The change in the density of the samples is determined on a photometric device with an optical logarithm introduced into the electrical circuit. The individual sensitivity of the patient to treatment is determined by the change in the degree of hemolysis relative to its normal values.

The method is illustrated by the following examples.

Example 1. Patient Pavlichenko N.V. enrolled in the pulmonary department of the 411 AVH of 09/14/86 with a diagnosis of chronic low-pressure pneumonia in the acute phase; chronic obstructive bronchitis; pulmonary heart disease II Art.

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On admission from the general analysis of blood acceleration of ESR up to 25 mm / h, leukocytosis with a neutrophilic shift to the left. In an X-ray examination of the lungs, there is an increase in a brochosuscular pattern of $ 5. “, a tear. Spirography - change in respiratory function of a mixed type. The study of the dynamics of acid hemocolysis of erythrocyte suspension was carried out at a ratio of the volumes of erythrocyte suspension and hydrochloric acid within the selected parameters of 20.3: 1.3 and incubation temperature of 37 ° C. 15

On the first day of inpatient treatment, hemolysis curves were recorded that went beyond the permissible rms values obtained in the study of hemolysis of healthy individuals.

individuals. The patient was prescribed a complex treatment, including antibacterial, bronchodilator, desensitizing drugs, physiotherapy. On the 3rd day of the treatment, the dynamics from the side of general clinical manifestations of the disease, hemogram data, pulmonary X-ray, spirography were absent. When repeated studies of acid hemolysis in the above parameters 30 ho, curves of the pathological profile were still recorded. The patient was replaced with an antibacterial drug (replacing the semi-synthetic antibiotic of the penicillin group with cephalosporin). 3 days after the change of the antibiotic, positive dynamics from the general blood test, pulmonary x-ray, and respiratory function were noted, while from the side of the acid hemopdolysis curves obtained with this ratio of agents and at an incubation temperature of 37.0 ° С, an increase in the degree of spherulation, indicating the restoration of the functional D5 state of erythrocyte membranes.

Example 2. Erythrocytes were studied for resistance to the effects of hydrochloric acid in the same patient Pavlichenko N.V. of Example 1, but in other modes. The erythrocyte suspension was mixed with hydrochloric acid in the ratio of 1.7: 0.7 and incubated at 36.6 ° C. In these regimens, the efficacy of treatment for nonspecific chronic lung disease was evaluated. The degree of hemolysis of erythrocytes was found to reflect a gradual improvement in the condition of the patient under the influence of the carried out thermonia. On the 7th fi day from the start of treatment, the hemolysis curve indicated a recovery in the state of the erythrocyte membranes, which meant an improvement in the clinical condition of the patient.

A significant increase in the degree of erythrocyte spherulation after adding hemolytic in a volume ratio of 19.8: 1.2 was not obtained due to a significant drop in optical density in the first 30 seconds accompanying the process of mixing agents. With a ratio of 20.5: 0.5, it was not possible to obtain sufficient repeatability of the profile of hemolysis curves due to difficulty in accurate dosage of small amounts of injected acid. A positive effect was achieved when conducting research in the temperature range of 36.6-37.0 ° C. At 36.4 and 37.1%, the positive dynamics from the profiles of the recorded curves was not observed.

The individual sensitivity to the treatment of COPD is more accurately determined using this method (experience) than the traditional (control) method (see tab. 1).

This method allows for earlier determination of individual sensitivity to treatment and, accordingly, the correction of treatment regimens, which reduces the duration of hospital stay by an average of 2.91 + 1.14 (see Table 2).

The method is simple to perform, does not require expensive reagents, and is available for poorly equipped laboratories.

Claims (1)

Invention Formula The method of determining individual sensitivity to treatment in chronic nonspecific lung disease by examining the patient's blood, which is indicated by the fact that, in order to accelerate and improve the accuracy of the method, the erythrocytes of the patient's peripheral blood are mixed with hydrochloric acid in a ratio of 20 + 0.3: 1 +0.3, the mixture is incubated at 36.6-37, 0 ° C, the degree of hemolysis of erythrocytes is measured by photometry, and the individual sensitivity to treatment is determined by the value of this indicator. i Table 1 table 2