SU1560108A1 - Method of determining oldness of depth - Google Patents

Abstract

This invention relates to forensic medicine. The purpose of the invention is to improve the accuracy of determining the time of death. Aqueous moisture is taken from the anterior chamber of the eye, then the fluorescence of the aqueous humor products of the eye chamber is measured at an excitation wavelength of 278 to 296 nm, and of emission from 320 to 370 nm. The duration of death is determined graphically from the ratio of the fluorescence intensities of the test object and the control. The use of the invention will improve the rapid diagnosis of the limitation of death. 1 il.

Description

The invention relates to medicine, namely to forensic medicine.

The purpose of the invention is to improve accuracy regardless of the time of death.

The method is carried out as follows.

Three to five microliters of water is taken from the deceased from the anterior chamber of the eye, without affecting the iris. Usually, the pupils of the dead are dilated and the iris does not interfere with the selection of water from the purest moisture. The fluorescence of the water products of the moisture chamber of the eye is measured at an excitation wavelength of 278 to 296 nm, and of emission from 320 to 370 nm. It was found that after the death of mammals, an increase in the fluorophore content occurs in the aqueous humor. There is a linear correlation between the fluorescence intensity of products F, reduced to the fluorescence of the standard, and the limitation period of occurrence of death T. An excitation wavelength of 293 nm and a fluorescence observation wavelength of 340 nm vary widely from 278 to 296 nm.

and from 320 to 370 nm, respectively, which does not affect the accuracy of determining the statute of limitations of death, since the principle itself is important - measurement of the content of chromophores that fluoresce in the same area as the aromatic amino acid tryptophan. The assessment of the content of such compounds is most accurately carried out by fluorimetric. In the work using the standard fluorescence 25 μm tryptophan solution. The standard fluorescence measurement conditions are the same.

After determining the fluorescence intensity F of the sample and standard E., based on previously obtained experimental dependencies between F / FCT and the time from the onset of death T, an estimate is made of how much time has passed since the death of a person or some other mammal.

The drawing shows how the fluorescence parameter of aqueous humor depends on the statute of limitation of death in humans. The linear nature of dependencies for more than 100 hours from the onset of

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death and a small scatter of experimental points makes it possible to accurately estimate the time of onset of death over wide time limits.

Example 1. M., 60 years old, who died from a traumatic brain injury, takes water to obtain moisture for analysis in an amount of 25 µl 99 hours and 10 minutes after the onset of death. Moisture is mixed in a quartz cuvette with 300 μl of distilled water. The suspension was fluorimetric at 340 nm (excitation 293 nm). The measured intensity of the sample and standard 30 srv. units and 0.9 sr. units respectively. The calculated parameter F / Er 33.33 rel. units, which according to the experimentally obtained dependence corresponds to 101 hours from the time of death. A comparison with the actually fixed period of limitation of death gives a method error of 2%. The analysis time takes about 1 minute.

Example 2. K., a 43-year-old who died from a traumatic brain injury, takes water for analysis in an amount of 25 µl after 33 hours and 15 minutes from the onset of death. Moisture is mixed in a quartz cuvette with 300 μl of distilled water. The suspension is fluorimetric at 370 nm (excitation is 278 nm). The measured intensity of the sample and standard 5.9 sr. units and 1.1 sm. units respectively. The calculated parameter F / FCT 5.36 rel. units, which, according to the experimentally obtained dependence, corresponds to 32 hours and 15 minutes after the onset of death. Comparison with the statute of limitations

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five

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The death stage gives a method error of 2%. Analysis time takes about 1. min.

Example 3. M., 22 years old, who died from a traumatic brain injury, takes water to obtain moisture for analysis in an amount of 25 µl 7 hours after the onset of death. Moisture is mixed in a quartz cuvette with 300 μl of distilled water. The suspension is fluorimetric at 320 nm (excitation is 296 nm). The measured intensity of the sample and standard 1.9 sr. units and 1.1 sm. units respectively. The calculated parameter F / Fcr is 0.44, which corresponds according to the experimentally obtained dependence of 6 hours and 10 minutes after the onset of death. A comparison with the actually fixed period of limitation of death gives a method error of 13.5%.

The table shows the accuracy of the known methods and the proposed method.

The use of the invention allows to improve the express diagnosis of the limitation of death.

Claims (1)

Invention Formula The method for determining the age of death by examining the aqueous humor of the anterior chamber of the eye, characterized in that, in order to improve accuracy regardless of the time of death, the fluorescence of aqueous humor and the fluorescence intensity ratio of the test object are measured at 340 + 20 nm compared with control at an excitation wavelength of 280 ± 20 nm graphically determines the duration of death. Activity of potassium ions in the heart cavity Chemiluminescence homogenates Liquor spectrometer Autographic tissue self-image analysis Auricle Fluorescence Fluorescence water nisty moisture 15 0-72 960-2160 0-100 120 60-90 20 30-60 30 2 # W. (rel. units)  behind . / oo T (t)