SU1503015A1 - Method of producing diagnosis of endogenic intoxication - Google Patents

Abstract

This invention relates to medicine. The aim of the invention is to improve the diagnostic accuracy and speed up the method. The method is characterized in that the erythrocytes are separated from the blood, the erythrocyte mass is placed in a test tube with a solution of a vital dye, for example, 0.025% methylene blue. Mix and incubate for 10-12 minutes. at room temperature, then centrifuged, the supernatant is spectrophotometrized and, when absorbed by red blood cells more than 35.69-38.55% of the dye, endogenous intoxication is diagnosed.

Description

This invention relates to medicine.

The aim of the invention is to improve the accuracy and acceleration of the method.

The method is carried out as follows.

4 ml of blood is taken from the patient's vein into a tube containing 1 ml of sodium citrate 3.8, mixed, and the red blood cells are separated by centrifugation for 10 minutes at 3000 rpm. Plasma is removed. Using a micropipette, transfer 1 ml of erythrocyte mass to a tube containing 3 ml. solution of the vital dye, for example, 0.025%, methi | Foam blue, prepared on physiological saline solution of 0.9 ° sodium chloride. Mix and incubate for 10-12 minutes at room temperature (18-22 C). Further, ci1: trifugate for 10 minutes at 3000 rpm. Transfer the supernatant to the cell of the photoelectrocalimeter or spectrophotometer. The optical density of the initial rastus and supernatant liquid is determined in units of the scale by the calorimetric method; o. :, for example, with the help of c :: --- ktro4yuometr: SF-26 (wavelength 6JO m), in relation to a physiological solution of 0.9% sodium chloride.

The amount of absorbed dye. I, A is calculated (in%) by the next form A, 00 -, B

c - optical density ghd-south

dye solution, extrication units; C — optical density of races g} eora

dye after incubation with erythrocytes of large, unit of extinction. Example 1. Patient B., 59 years old, is undergoing treatment in the intensive care unit with a diagnosis of E-morrhagic pancreatic necrosis, DI1) fuzny peritonitis, post-laparotomy, drainage of the abdominal bands U i

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ty and omental cyMKt ;. When the TV TV .JICNIA is in a state of separation, the consciousness is inhibited, the face is more than peaceful, 1 temperature 38 ° C, tachycardia is up to 112 in mil. Mrterinalpe; .- h. 100/60 mm Hg Along with the general. Investigations into the presence of endogenous toxicity are carried out. Erythrocytes are isolated from the venous patient for 10 minutes. incubated in 0.025% methylene blue solution. After re-separation of the erythrocytes, the optical densities of the initial dye solution (B) and the dye solution after incubation (c) on an SF-26 spectrometer are determined. Get the following indicators:, 9; . The formula calculates the amount of absorbed dye L 100-06:: 1.9100 6b, 4%. Erythrocytes of healthy people absorb up to 37.12 + 1.43% of the dye.

The data obtained indicate the presence of endogenous intoxication. As a result of the treatment, the patient's condition improved. The control determination of the amount of absorbed dye according to the proposed method revealed a decrease in this indicator, which at discharge was 40.2%, which is close to that of healthy people.

Example 2: Sick G., 26 years old, is undergoing treatment in the intensive care unit with a diagnosis of bronchial asthma, infectious and allergic form, asthmatic status of 3 degrees. At the height of the asthmatic status, the amount of absorbed methylene blue by red blood cells of venous blood is determined to be 80.2%, which indicates a pronounced endogenous intoxication. After 1 day. After stopping the status of JTOT, the indicator is 51.2%. Such a significant decrease in endogenous intoxication is clinically accompanied by an improvement in the condition of the patient.

Example 3: Patient K., 69 years old, is undergoing treatment in the intensive care unit of JHHH with a diagnosis of ischemic heart disease, acute transmular, anterior-widespread myocardial infarction, atherosclerosis of the aorta, coronary cardiosis of H,. acute myocardial infarction

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- {. vpoHfMu, endogenous intoxication-; and. By the number of absorptive hl of -v, I and 11; the new blue was ((), T. In the subacute period, this period is reduced to 49.5%, which is positive clinical disease and electrocardiographic dynamics of the disease.

The proposed method for the diagnosis of endogenous intoxication has been applied in 62 patients with various diseases. In all patients, there is a statistically significant erythrocyte uptake of vital dye compared with erythrocytes of healthy people, which indicates a high degree of sensitivity. Well, in one healthy donor the amount of absorbed dye did not go beyond the established norm, which indicates a high specificity of the method. The accuracy of the random method is 4-5 times Bijiiiic TO4HOCTV of a known method.

With the help of the OrOlt, we propose. The method allows to diagnose the endogenous int ((sikachiyuyu with gts mo1tsi only sobs.t0

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without. nocTcii) otmero C i oh clos ma te ,, that allows significantly y; 111; gti; s procedure is cleared; a 1ip on C; ABNIUM with the known method. The influence on the results of the study of the conditions and periods of storage of do-yrs is excluded: oi croppa, possible immunological reactions between erythrocytes donor ;; and (iMMOR patient that POVI-

accuracy of diagnosis of endogenous intoxication. In addition to TCI, the predagay, y. Method is much faster than the known one (33-40 minutes versus 2.5-3 hours), which expands the possibilities of its use for the rapid diagnosis of endogenous intoxication in various diseases. The dynamics of the studied index corresponds to the typical data, which can serve as a test for the effectiveness of the treatment being carried out and have not only a diagnostic, but also a prognostic value ;.

Claims (1)

Claims of the invention A method for diagnosing endogenous intoxication by means of a blood test, characterized in that, in order to increase the accuracy and speed up the method, the erythrocyte is subjected to the test (, 1, mix them with a solution of ne incubated for 10–12 min at room temperature, diagnosed with endogenous intura and toxication when erythrocytes are absorbed by erythrocytes.