SU1495371A1 - Method of preparing sugar-containing media for growing feed yeast - Google Patents

Abstract

The invention relates to the microbiological industry and is a method for preparing sugar-containing media for growing fodder yeast. The purpose of the invention is to increase the yield of yeast. Wood hydrolyzate or alcoholic bard is purified from inhibiting impurities by ozonation. The amount of ozone required for processing is calculated according to the formula D = 0.054 C _f + 0.05 C _omf + 0.03 C _lgv1 + 0.02 C _lgv2 , where C _f , C _omf , C _lgv1 , C _lgv2 are furfural concentrations, hydroxymethylfurfural, the first and second fractions of lignohumic substances. Ozonation is carried out at a temperature of from 30 to 60 ° C. 2 tab. hm pIoJcoRJ.a226

Description

one

(21) 4238382 / 28-13

(22) 05/04/87

(46) 23-07..89. Bul P 27

(71) Scientific and production hydrolysis association Hydrolysis

(72) A.A. Yavich, A.A. Shupl, E.E..Dmitriev, I.M.Golubkov, V.V.R. bov, V.G.Ponomarenko, E.A.Evelson and V.A. .Rits (53) 577.15 (088.8)

(56) USSR Author's Certificate No. 209379, cl. C 12 N 1/22, 1965,

Likhovid R.D., Korolkov I.I. To the question of the purification of wood hydrolysates by dispersing gaseous oxidizers: Coll. proceedings VNIIGydroliz, 1969, t. 18, p. 120

(54) METHOD OF PREPARATION OF SAMSORFETING MEDIA FOR EXTRACTING CORTEX YEARS

(57) The invention relates to the microbiological industry and is a method for preparing sugar-containing media for growing fodder yeast. The purpose of the invention is to increase the yield of yeast. Wood hydrolyzate or distillery bard is purified from inhibiting impurities by ozonation. The amount of ozone required for treatment is calculated by the formula, 054 Сф + 0.05 Со cf + + 0, ОЗСдг, + 0.02 С, rw ,,, where С,,

 lgv

SLF concentration of furfur

la,. oxymethyl furfural, the first and second fraction of lignohumic substances. Ozonation is carried out at a temperature of from 30 to. 2 tab.

The invention relates to the microbiological industry, in particular to a method for preparing sugar-containing media, such as hydrolysis, for growing fodder yeast by purification from inhibiting impurities such as furfural, oxymethylfurfural, ligno-humic substances that reduce the yield of yeast.

The purpose of the invention is to increase the yield.

The method is carried out as follows.

The sugar-containing medium, for example, wood hydrolyzate or distillery bard, is treated with ozone at 30 - 60 ° C. The amount of ozone is determined by the formula ,, 05 CoM f - 0.,. + 0.02 Cdge, 1,. .

 where b is the amount of ozone, g / l.

I

vra

Agwy

concentrations of furfural, hydroxymethylfurfural, the first and second fractions of ligno-humic substances, respectively, g / l.

The first fraction of ligno-humic substances are substances that are quickly oxidized.

SO eaten

oo j

3149

ozone, i.e. the rate constant of their oxidation, the rate constants above) the breakdown of sugars, the second fraction consists of substances having an oxidation rate constant, which is smaller than the rate of decay of the sugars, the ozone-treated medium is used to enhance fodder yeast. On media treated with ozone, the amount of which is determined by formula (1) in the indicated temperature range; maximum yeast yield is observed. When the temperature of the solution is below 30 and 60 ° C, more ozone is required to provide the same degree of purification, which leads to the decomposition of sugars. With more than 10% of the decomposition of sugars, the degree of purification of the medium is reached and the corresponding nature of the amount of yeast cannot be compensated. A decrease in yeast biomass yield due to a decrease in sugar concentration. Empirically derived proportionality ratios between the amount of ozone and the concentration of inhibitory impurities in formula (1) are optimal for calculating the amount of ozone used for processing.

Example 1. The wood hydrolyzate (50% hardwood and 50% coniferous) is neutralized with lime milk to pH 3.5 at 90 ° C, held at this temperature for 1 hour, the gypsum is separated by filtration, the pH is adjusted to 4.2 25 The% ammonia solution in water. The concentration of furfural and hydroxy- 1-methylfurfural is determined spectrophotometrically. To determine the concentration of the first and second LGV fractions, a 100 ml sample of the medium is treated with activated carbon, stirred and filtered and washed with water. Then the precipitate is washed with 100 ml of ethanol to obtain the first fraction of LGV,

and 40 ml of 5% -Hiro alkaline solution, obtaining a second LGV fraction. The amounts of LGV in the first and second fractdas are obtained after evaporation of the alcohol and drying to constant weight.

5 liters of hydrolyzate, adjusted to pH 4.2, are treated with ozone in a column apparatus. The amount of ozone is determined by the formula (1). The treated hydrolyzate was used to digest the Candida scottii KS-2 yeast at 38-39 ° C, pH 4.2. The results are presented in table 1.

PRI mme R 2. The method is carried out according to Example 1, but the distillery bard is subjected to a scrub. The results are presented in table 2.

Thus, the proposed process of cleaning sugar-containing solutions from inhibiting impurities allows increasing the yield of cortical yeast by 15-50% while reducing the amount of ozone used.

F o rmu l invention

The method of preparing sugar-containing mediums for growing fodder yeast, providing for their ozonation, characterized in that, in order to increase the yield of yeast, before ozonization, concentrations in furfural medium, oxymethylfurfural, lignohumic substances of the first and second fractions are determined, while ozonization is carried out at 30 - 60 C, and the amount of ozone is calculated by the formula

, 054С, + 0,05Со „-Y.OZSdgb, +

+ 0,02Сдгв,

where ssr, sd (p,

Slgv, Slgv, 2. concentrations, furfural, oxymethylfurfural, first and second fractions of lignohumic substances, respectively.

Table 1

Table 2

Claims (1)

Claim A method of preparing sugar-containing media for growing fodder yeast, providing for their ozonation, characterized in that, in order to increase the yield of yeast, concentrations of furfural, hydroxymethylfurfural, lignohuminic substances of the first and second fraction are determined in the medium before ozonation, while ozonation is carried out at 30-60 C, and the amount of ozone is calculated by the formula D = O, O54C _T + O, O5C _OM , + O, O3C _{M & 1} + + 0.02С dgvg> where Οφ, 0 _{ΟΛ5 <ρ} , ^with lgv, “C. lggbgg. “Concentrations of furfural, hydroxymethylfurfural, and the first and second fractions of ligno-humic substances, respectively. Table 1 Example  ~ t - _g ------ - -------- - -------------- The composition of the neutralizer after treatment Yeast yield from PB,% The total amount of yeast _in g / l t ^and s D, g / l PB,% Sugar, g / l Of, g / l ^ 0M <? ’ g / l Sdge. G / l G-lgv * "g / l 1 (cont role) 3.2 29.5 0.8 1.05 1,2 0.6 31 9.9 2 thirty 0.143 3,1 2.73 0.52 0.8 - 0.55 48 14.9 3 40 0.143 3,1 2.74 0.54 0.78 - 0.54 48 14.9 4 60 0, 143 3,1 27.4 0.50 0.79 - 0.55 475 14.7 5 20 0, 143 3,1 27.5 0.59 0.89 - 0.60 46 14.2 6 70 0.143 3,1 27.4 0.61 0.92 - 0.60 45.5 14.1 7 40 0, 100 3.15 28.0 0.65 0.98 0 _l 6 45 14.17 8 40 0,200 2.95 2,51 0.46 0.65 - 0.49 49 14,4 9 40 0,500 2.70 2,32 0.38 0.55 - 0.41 51 18.6 10 (famous) 20 2 2.1 18,4 0.28 0.44 0.29 54 11, 3 20 4 1.8 16.0 0.21 0.33 . - 0.21 56 10.1 Table 2 Example 0 t, s D, g / l g ...... ^L '' ..... '' Composition of stillage after ozone treatment Yeast yield from PB,% Yeast count g / l PB,% Sugar, g / l S ,, g / l Oomt "g / l Odgv | g / l ^With LG6 „> g / l 1 (cont role) 1,1 0.95 0.15 0.6 0.5 0.4 44 4.8 2 thirty 0.06 1,0 0.87 0.1 .0.45 - 0.37 62 6.2 3 40 0.06 1,0 0.88 0.105 0.45 - 0.38 61.5 6.15 4 60 0.06 1,0 0.87 0.1 0.46 0.37 62.5 6.25 5 20 0.06 1,0 0.89 0.115 0.48 - 0.4 58.5 5.85 6 70 0.06 1, -0 0.90 0.12 0.48 - 0.4 58 5.8 7 40 0,03 1.05 0.92 0.12 0.50 - 0.4 57 6.0 8 40 0.1 0.92 0.82 0.85 0.4 - 0.33 64 5.9 9 40 0.2 0.83 0.72 0,075 0.36 - 0.30 66 5.5 10 (famous) 20 2 0.6 0.53 0.056 0.29 __ 0.25 69 4.1 20 4 0.42 0.35 0.04 0.23 - 0.21 71 3.0