SU1491885A1 - Strain of lactobacillus acidophilis bacteria for normalizing the microflora in vaginal microbioceosis disturbances - Google Patents

Abstract

FIELD OF THE INVENTION The invention relates to medical microbiology, in particular, to the manufacture of medicinal preparations - dentics. To prepare the bacterium, the strain of bacteria LACTOBACILLUS ACIDOPHILUS CMPM B-3325 is used. The purpose of the invention is to increase the adhesive ability of bacteria. The increased adhesive ability of the strain helps to accelerate the normalization of microflora in violation of the vaginal microbiocenosis. 2 tab.

Description

The invention relates to medical microbiology, and in particular to the production of curative: preparations - dentics.

The aim of the invention is to increase the adhesion ability of bacteria included in the composition of bacterium,

The bacterial strain Lactobacillus acidophilus was obtained from the initial strain AL-28 by selection. The strain is deposited in the central Museum of Industrial Microorganisms of the Institute of Scientific Research Institute of Geotechnology under the number of CMMP B-3325 and is characterized by the following prizes.

Cultural and morphological features. Rod-shaped cells, and thin, non-spore.

Gram-positive, indistinguishable. On dense medium, MDCs form round colonies, the profile is convex, white, homogeneous, easily removed by a loop from the surface of the agar, edges smooth, opaque, without gloss. Pigment does not form. In a liquid medium, MPC grows diffusely throughout the tube (except for the aerobiosis zone in the upper layers of the nutrient medium), a precipitate is formed at the bottom, easily broken when shaken.

Skimmed milk - on the second day after sowing, the milk coagulates to form a tight clot and separate the whey. On a liquid synthetic medium H.Haenel in the form of a uniform turbidity and a small

00 00

SP

draft; the number of viable cells at the same time reaches 10 -10 cells / / MP. The first passages of the strain produce abundant growth on Blaurock’s liquid medium for bifidobacteria; in post-seeding, the growth of the strain may be depleted. In semi-liquid (0.7%) agar, Blaurock (when seeded in test tubes with 9 mp of medium) forms slightly convex disc-shaped colonies.

Physiological and biochemical properties. Anaerobic, on a solid medium grow after replacing the air WITH. Maximum growth at 37.5 ° C. The optimum pH is 6.2-6.4.

Ferments sucrose, maltose, galactose, fructose, lactose, raffinose; does not ferment lyxose, xylose.

mannitol, rhamnose, dulcite, citrulline, ribose, sorbitol.

Acid formation when grown in skimmed milk 90 (according to Turner).

Laboratory testing showed non-pathogenicity of L.acidophilus A-A for experimental animals and humans.

Example 1. A strain of lactobacilli is infused on a liquid MFC medium (pH 6.2–6.4) for 36 hours in a thermostate in bacteriological tubes containing at least medium. The optimal temperature is 37.5 C. Sowing dose 1 ml of 36-hour culture

Hepatic

decoction

Twin 80

100.0 ml 1.0 ml

Distilled water Up to 500.0 ml

Hydrolyzate

milk 500,0 ml

rI6,2

Sterilization 20 min at 0.5 atm.

On the basis of a lyophilized preparation, vaginal suppositories can be prepared.

The biomass of bacteria containing W-Yu cells is mixed with 30 g of cocoa butter and 2.0 g of lanolin. The resulting homogeneous mass is rolled out and laid out in a special metal dispenser, with which 30 candles can be obtained from the initial amount of biomass. Candles are stored in cellophane wrappers in a refrigerator. In addition, the drug can be applied per vaginum using tampons or instils. At the same time, the ampoules are diluted with 1-2 ml of boiled or distilled water, as well as with saline.

Take p2. Studying the effect of lactobacilli on the normalization of vaginal microflora in women.

Under observation there were women of different ages with vaginal dysbiosis: the level of lactobactus

After that, the suspension of microorganisms pH was from 10 to less than 10 cells / centrifuged at 300 rpm for 1 g. At the same time, the amount of staphyllion is 15 min. The precipitate is resuspended in preservative and the mixture is broken into ampoules, and then subjected to freeze drying.

Preservative - LF containing 10% sucrose and 1% gelatin, pH 6.0.

After freeze-drying, at least 10 viable cells should be contained in one ampoule containing 1 dose of the preparation.

The composition of the MFC medium for lactobacillus growth:

MgSO.iuu mg

Cytheine (HC1)

Ammonium citrate

Sodium acetate

Glucose

MnSO

Peptone

Yeast

avtopizat

(Di fso)

2 g

200 mg 200 mg 2 g 5 g 20 g 37.9 g 10.0 g

5.0

pH ranged from 10 to less than 10 cells / / g. At the same time, the amount of staphylo

cocci amounted to 10-10 cells / g, yeast 1C -10 cells / g and enterobacteria 10 cells / g, 8 women received topical daily for 10 days, 1 gi 10 cells / g L.acidophilus. The other 8 women did not receive the bacterial preparation. On the 12th day after the start of treatment in women who received -L.acidophilus, the number of lactobacilli increased to cells / g (i.e., 10 times or more). The number of staphylococci, yeast and enterobacteria, on the contrary, decreased by 100, 10 and 10 times, respectively. In women who did not receive topically during the treatment of L.acidophilus, the severity of vaginal dysbiosis during this period of observation remained at the same level.

Claims (2)

The results are shown in table 1 and 2. The formula of the invention Bacterial strain Lactobacillus acido-philus CMPM B-3325 (Central Museum of Industrial Microorganisms This SIIIg enetnk) used to prepare bacterium for norm1491885 lizatsii No. 1 of a croflora at disturbance of a microbiocenosis of a vagina. Table 1 table 2