SU143963A1 - Method for isolating crystalline sodium salt of 6-aminopenicillanic acid from culture fluid obtained by deep fermentation of the microorganism Pemcillium chrysogenum - Google Patents
Method for isolating crystalline sodium salt of 6-aminopenicillanic acid from culture fluid obtained by deep fermentation of the microorganism Pemcillium chrysogenumInfo
- Publication number
- SU143963A1 SU143963A1 SU725173A SU725173A SU143963A1 SU 143963 A1 SU143963 A1 SU 143963A1 SU 725173 A SU725173 A SU 725173A SU 725173 A SU725173 A SU 725173A SU 143963 A1 SU143963 A1 SU 143963A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- aminopenicillanic acid
- microorganism
- chrysogenum
- pemcillium
- sodium salt
- Prior art date
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
Изобретение касаетс способов выделени 6-аминопеннциллановой кислоты в виде ее натриевой соли из культуральной жидкости, получающейс при глубинной ферментации микроорганизма Penicillium chrysogenum .The invention relates to methods for isolating 6-aminopenncyllanic acid in the form of its sodium salt from the culture fluid obtained by deep-fermentation of the microorganism Penicillium chrysogenum.
Известные способы не позвол ют выделить препарат 6-аминопенициллановой кислоты из культуральных жидкостей, полученных путем глубинной ферментации в кристаллическом виде.The known methods do not allow the preparation of 6-aminopenicillanic acid to be isolated from the culture fluids obtained by deep fermentation in crystalline form.
В данном изобретении этот недостаток устран етс тем, что наход щуюс в нативном растворе 6-аминопенициллановую кислоту с помогцью переносчика экстрагируют при ,8 органическим растворителем . Экстракт под1целачивают и реакстрагируют из него 6-аминопенициллановую кислоту водой. Из полученного реэкстракта удал ют воду и перекристаллизовывают готовый ирепарат, например, из этилового спирта.In the present invention, this disadvantage is overcome by the fact that 6-aminopenicillanic acid in a native solution is extracted with a carrier solution, with 8 organic solvents. The extract is pickled and 6-aminopenicillanic acid is reacted from it with water. Water is removed from the resulting reextract and the final preparation is recrystallized, for example, from ethanol.
Способ основан на свойстве 6-аминопенициллановой кислоты, наход щейс в кислом нативном растворе в виде катиона, образовывать соли с органическими соединени ми, обладающими рко выраженным кислотным характером. В качестве таковых примен ют сульфокислоты жирного или ароматического р да, однозамещенпые эфиры органических сульфокислот и прочие. Указанные соединени выполн ют роль переноечиков 6-аминопенициллановой кислоты из водной среды в органическую . Органическа среда может быть образована малорастворимыми в воде спиртами, сложными эфирами или другими подход щими растворител ми. Реэкстракцию 6-аминопенициллановой кислоты в водную среду производ т в щелочной среде. В результате проведени процесса экстракции и реэкстракции происходит как концентрирование раствора 6-аминопенициллановой кислоты, так и удаление большого количества примесей, содержавшихс в нaтивIю растворе. Из полученного реэкстракта удал ют воду и обрабатывают сухой препарат спиртомThe method is based on the property of 6-aminopenicillanic acid, which is in an acidic native solution in the form of a cation, to form salts with organic compounds that have a clearly pronounced acid character. As such, fatty or aromatic sulfonic acids, monosubstituted esters of organic sulfonic acids, and others are used. These compounds play the role of transferring 6-aminopenicillanic acid from an aqueous to an organic medium. The organic medium can be formed by water-soluble alcohols, esters, or other suitable solvents. Extraction of 6-aminopenicillanic acid into the aqueous medium is carried out in an alkaline medium. As a result of the extraction and reextraction process, both the concentration of the 6-aminopenicillanic acid solution and the removal of a large amount of impurities contained in the inactive solution occur. Water is removed from the obtained reextract and the dry preparation is treated with alcohol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU725173A SU143963A1 (en) | 1961-04-05 | 1961-04-05 | Method for isolating crystalline sodium salt of 6-aminopenicillanic acid from culture fluid obtained by deep fermentation of the microorganism Pemcillium chrysogenum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU725173A SU143963A1 (en) | 1961-04-05 | 1961-04-05 | Method for isolating crystalline sodium salt of 6-aminopenicillanic acid from culture fluid obtained by deep fermentation of the microorganism Pemcillium chrysogenum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU143963A1 true SU143963A1 (en) | 1961-11-30 |
Family
ID=48299688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU725173A SU143963A1 (en) | 1961-04-05 | 1961-04-05 | Method for isolating crystalline sodium salt of 6-aminopenicillanic acid from culture fluid obtained by deep fermentation of the microorganism Pemcillium chrysogenum |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU143963A1 (en) |
-
1961
- 1961-04-05 SU SU725173A patent/SU143963A1/en active
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