SU1397810A1 - Method of quantitative analysis of sodium thiopental - Google Patents

Description

(21) 416370A / 28-0

(22) 12/16/86

(46) 05.23.88. Bul No. 19

(71) Zaporozhye Medical Institute

(72) S.G.Solomonova, S.S.Artyemchenko, V.V. Petrenko, N.P. Borova

and V.M. Nichvoloda

(53) 543.42.03 (088.8)

(56) USSR State Pharmacopoeia, 10th ed. - M .: Medicine, 1968, p. 693.

Korenman I.M. Photometric analysis. M .: Himi, 1975, p.279.

(54) METHOD FOR QUANTITATIVE DETERMINATION OF SODIUM TIOPENTAL

(57) The invention relates to analytical chemistry, in particular to the quantitative determination of sodium thiopental 5-ethyl-5- (2-amyl) -2-sodium thiobarbiturate with anhydrous sodium carbonate. The purpose of the invention is to increase the sensitivity and simplify the method. The determination is carried out by treating a solution of the analyzed sample of sodium thiopental with a solution of N-toluene-sulfonyl-2- (2,4-diketo-4-methoxy-iso-butyl) -, 4-naphthoquinone imine in acetone with subsequent photometry of the resulting solution. The sensitivity increases by 19-47 times, the analysis time is reduced by 2.3-2.6 times in comparison with the known method. 2 tab.

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This invention relates to analytical chemistry, in particular to methods for the quantitative determination of 5-ethyl-5- (2-amyl) -2-sodium thiobarbiturate with anhydrous sodium carbonate-sodium thiopental, used in medical practice as a means for intravenous anesthesia.

The purpose of the invention is to increase the sense of vitality and simplify the method.

Example. Quantitative determination of sodium thiopental in substance.

An exact weight of the drug in the range of 0.01-0.02 g is dissolved in water in a 50 ml volumetric flask and made up to the mark with water. 1 ml of the solution obtained is placed in a volumetric flask with a capacity of 25 ml, 5 ml of a 0.25% acetone solution of N-toluenesulfonyl-2- (2-4-dicoteto-A-methoxyisobutyl) -1,4-naphthoquinohyl are added. After 10 min, the volume of the solution was adjusted to the mark with acetone. In parallel, experiments were carried out with a standard solution of sodium thiopental and a background solution. The optical density of the analyzed solutions is measured against the background of the control using a spectrophotometer at 610 nm in cuvettes with a layer thickness of 1 cm.

The calculation of the quantity of sodium thiopental in terms of 5-ethyl-5- (2-amyl) -2-thiobarbituric acid is given by the formula

WITH,%

0 50-25

de D is the optical density of the analyzed solution; optical density of the standard solution; concentration of a standard spectrophotometric solution (0.0012 g in I00 ml) sample, g; layer thickness, see

D. C. P 1

The results of quantitative determination of sodium thiopental in the substance are given in Table 1.

The results of the comparative analysis of the proposed and known methods are given in table 2.

Table 2

Time of analysis, min

Sensitive, mg / ml photometric solution

35-40

0.15-0.75

15

0,008-0,0160

0

Claims (1)

As can be seen from the table. 2. The proposed method exceeds the known sensitivity by 19–47 times in magnitude 19–47, the analysis time is reduced by 2.3–2.6 times. Invention Formula The method of quantitative determination of sodium thiopental by treating an analyzed sample with an organic reagent followed by photometry of the resulting solution, characterized in that, in order to increase the sensitivity and simplify the process, H-toluenesulfonyl-2- (2-4) is used as a reagent. -diketo-4-methoxyisobutyl) -1,4-naphthoquinone imine and the treatment is carried out in acetone.