SU1394098A1 - Method of diagnostics of glomerular hematuria - Google Patents

Abstract

 This invention relates to medicine, more specifically to nephrology. The purpose of the invention is to increase the accuracy of the method. For this, the patient's urine sample is centrifuged at 1500 rpm for 5 minutes. Separate the supernatant fraction and precipitate. Red blood cells of the patient are added to the supernatant at the rate of 1 drop of blood / 9.5 ml of liquid. Swabs are prepared from the sediment and the supernatant, the floxin is permeated in the buffer solution, covered with a glass slide, and microscopic. If the shape and size of the erythrocytes in the smear are changed from the sediment in comparison with the smear from the supernatant fraction, it is done to indicate the presence of glomerular hematuria.

Description

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This invention relates to medicine, in particular to nephrology.

The purpose of the invention is to show the exact point of the method.

The method is carried out as follows.

Take a sample of 10 ml of fresh urine. The sample is centrifuged at 1500 rpm for 5 minutes. The supernatant fraction and sediment are separated. The patient's red blood cells are added to the supernatant fraction at the rate of 1 drop of blood per 9.5 ml of liquid.

Samples are prepared from the precipitate and supernatant liquids, which are stained with a solution of phloxin in a buffer solution, coated with a glass and microscopically. Diagnose the presence of hemuerular hematuria by changing the shape and size of erythrocytes in a smear from a sediment compared with a smear from a supernatant fraction, which makes it possible to exclude the release of traumatic erythrocytes (non-globular hematuria),

I Example 1, A patient has headache, weakness, HELL 180–110 mm Hg, Art. In urine: relative density 1,018, protein 4,95 g / l, in sediment 8-10 erythrocytes in the visual field, hyaline cylinders.

In the study of sediment, erythrocytes were found with a changed size and shape. In a smear from the supernatant fraction, the erythrocytes are not changed, the glomerular Yaturi syndrome is diagnosed, chronic glomerulonephritis

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Example 2, A patient has a complaint of pain in the pig, weakness, frequent urination, BP 120-80 mm Hg, Art. In urine: relative density 1,012, no protein, leukocytes in the sediment, erythrocytes 10-15 in the visual field. In the study of erythrocytes, changes in their size and shape were not detected in the sediment. In the smear from the supernatant fraction, the erythrocytes were also not changed. Diagnosed with non-glomerular hematuria, urolithiasis.

Thus, the proposed method allows to increase the accuracy of diagnosis of glomerular aturia hem from non-glomerular to 100%. The specificity of the method also increased 2 times. The method makes it possible to completely eliminate the accidental appearance of a traumatic blood from systemic kidney damage.

Claims (1)

Invention Formula A method for diagnosing glomerular hematuria by microscopic examination of urine sediment, which is due to the fact that, in order to show the accuracy of the method, a supernatant fraction is additionally examined, to which peripheral blood erythrocytes are added and the diagnosis of glomerular hematuria is compared in size and forms of red blood cells in the sediment and supernatant fraction. fenaKTop N, dumb Compiled by L, Stebaeva Tehred M. Morgental - Order 2213/39 Circulation 847 VNIIPI USSR State Committee for inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab ,, d, 4/5 Proofreader Oh, Kravtsov Subscription