SU1392096A1 - Method of controlling process of cultivation of aerobic microorganisms - Google Patents

Abstract

The invention can be used in the production of amino acids and enzymes. The aim of the invention is to reduce the loss of the target product with the emission of foam during operation of the apparatus with the maximum possible filling of the gas-liquid medium (GLM). For this purpose, the difference in hydrostatic pressures is determined by sensors 2 and 3 installed along the height of the apparatus 1 in the aeration zone. The difference in block 5 determines the actual average value of the SHC density. In addition, the hydrostatic pressure difference of the SHC is determined by sensor 2 and the gas pressure above the liquid by sensor 4, according to which in block 6 the calculated value of the SHC density is determined under the condition MaKCHMaJibHO of the possible filling of the apparatus with foamed medium. The control unit 8 controls the flow of the foam carrier through the valve 9 depending on the difference between the calculated and actual values of the SHC density. The calculated density value is set by the setting device 7 and automatically adjusted in block 6 depending on the change in the volume of the loaded liquid medium. 1 il. § (L

Description

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The invention relates to methods for controlling processes that are accompanied by foam formation during the cultivation of aerobic microorganisms, and can be used in the microbiological, food and medical industry.

Yri / cultivation of microorganisms due to their vitality fO and air supply for the azelation of the medium

I occurs the distribution of air bubbles and the emitted gas in the liquid

I will form a gas-liquid medium.

i Depending on the content of gases in this medium, its density and volume in the apparatus change.

g Dp exclude product losses with

: by discharging foam, the process of cultivating I of aerobic microorganisms is expediently carried out so that the upper level of oena does not exceed the maximum

1 of the permissible filling value of a specific machine design.

The aim of the invention is to reduce the loss of product with the emission of foam by adjusting the supply of antifoam agent according to the relative density of a given density of a gas-liquid medium, determined in the measurement zone to different MINIMUM values.

The drawing shows a diagram explaining the process control method.

The device 1 is equipped with pressure sensors 2-4, of which sensors 2 and 3 are installed in the aeration zone of the liquid and sensor A above the liquid level.

The outputs of sensors 2 and 3 are connected to the inputs of the unit 5 that converts the difference of hydrostatic pressures into a signal, 1 is the proportionality of the average density of the gas-liquid medium in the measurement zone, which is limited in height by the apparatus with the installation levels of sensors 2 and 3.

Sensor A and sensor 2 are connected to the inputs of the unit 6 for converting the difference in hydrostatic medium pressure at the level that the sensor 2 is installed and the gas pressure above the liquid into the signal is proportional to the density of the gas-liquid medium as its volume increases to the maximum permissible filling of the apparatus.

On one of the inputs of the conversion unit 6 is served from the output of sensor i

LeMioi NZ conditions udein its volume is 30 constant signal setting

in the measurement zone to different MINIMUM values.

The drawing shows a diagram explaining the process control method.

The device 1 is equipped with pressure sensors 2-4, of which sensors 2 and 3 are installed in the aeration zone of the liquid and sensor A above the liquid level.

The outputs of sensors 2 and 3 are connected to the inputs of the unit 5 that converts the difference of hydrostatic pressures into a signal, 1 is the proportionality of the average density of the gas-liquid medium in the measurement zone, which is limited in height by the apparatus with the installation levels of sensors 2 and 3.

Sensor A and sensor 2 are connected to the inputs of the unit 6 for converting the difference in hydrostatic medium pressure at the level that the sensor 2 is installed and the gas pressure above the liquid into the signal is proportional to the density of the gas-liquid medium as its volume increases to the maximum permissible filling of the apparatus.

To one of the inputs of the conversion unit 6 is supplied from the output of the sensor.

MA to the maximum allowable level

For this purpose, the difference between the hydrostatic dissociation of the gas-liquid srein and the height of the pressure, the difference between the hydrostatic pressure of the fluid and the pressure of the gases above the fluid is determined. The regulation of the supply of the pen-detector is performed depending on the difference between the calculated and actual values of the density of the gas-liquid medium. .

In this case, the actual values of BODY are determined by the difference in the hydrostatic pressures of the gas-liquid medium over the height of the apparatus and the calculated value by the difference between the pedrostatic pressures of the liquid medium and the pressure of the gases above the liquid.

This definition of the calculated density value is due to the fact that with different loading of the apparatus with liquid medium and additional feeds of various components during the operation of the apparatus, an increase in the volume of the gas-fluid medium during foaming up to the maximal filling capacity of the amis. accompanied by: a change in the average density of the gas-liquid medium

where -

g liquid-liquid medium provided that the apparatus is fully loaded

The signal from the output of the block b enters the regulation of block 8 as a task automatically corrected in response to the variable volume of the medium in the apparatus during the operation, for example, by feeding the reagents for pH and so on.

The output of the regulating block® 8 yoedog veins with the executive mechism of Pan 9 of the supply of antifoam agent.

The method is carried out in the following manner.

When the apparatus was filled with medium and sowing material, which were combined with the definition of tactical instructions, races; by, determine the average value of n from the axis of the gas-liquid medium when filling the atmosphere with J, for example, to its full volume;

 . -

S J.f.s: iOz9YuiYs) b.

GSuJ

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the medium density of the gas-liquid medium under the filling conditions of 0.9 Ud,

KG / M S

fii Pr the DENSITY of the liquid medium and

gas, kg / m;

Oud ,, W., - the total volume of the apparatus and the volume of fluid in the apparatus, m,

A signal proportional to the obtained value of p. Is inputted to block 6 through the air sensor 7.

Since the sensors 2 and 3 are installed along the height of the apparatus at a certain distance from each other, the measurement density is average in volume, limited by the base of measurement.

At the beginning of the process, the signal at the output of block 5 is greater than the reference signal from the output of block 6, therefore, the regulating block 8 does not give a signal to supply the detector through the valve 9,

As biomass grows and foam forms, the volume of the gas-liquid mixture increases and its density decreases.

When the average density in the measurement zone decreases to the specified value, block 8 outputs a signal to the supply of the antifoam agent.

The destruction of the foam is accompanied by a sharp decrease in the level of the gas-liquid medium and an increase in its density, as a result of which the block B causes the signal to stop the supply of the antifoam agent. Depending on the setting of the parameters of the adjusting dego block 8, the permissible change in density and, accordingly, the change in level are established.

As a result of the implementation of this method in comparison with the base object in the production of L-lysine with equal loads of liquid medium apparatus with a capacity of 100 m, the yield of culture fluid increases by an average of 2.5 m while reducing the specific consumption of antifoaming agent from 0, 118 to 0,056 per ton finished product.

Claims (1)

Invention Formula A method for controlling the cultivation of azobic microorganisms involving the regulation of gas pressure in the apparatus and the supply of antifoam agent, characterized in that, in order to reduce product losses with foam release, the difference between the hydrostatic pressures of the gas-liquid medium along the height of the apparatus and the difference of the hydrostatic pressure of the liquid medium and the pressure of gases above the gas is determined depending on the difference between the calculated and actual values of the density of the gas-liquid environment, and. the latter is determined from the difference in hydrostatic pressures of the gas-fluidic medium along the height of the apparatus, and the calculated value is determined from the difference between the hydrostatic pressure of the liquid medium and the pressure of gases above the liquid.