SU1057933A1 - System for automatic control of process of growing micro-organisms - Google Patents

Abstract

THE AUTOMATIC URALATION SYSTEM OF THE CULTIVATIVE MICROORGANISM PROCESS, containing a pH sensor connected through the pH regulator to the actuator, is installed -: - I and determine the growth rate of microorganisms, and an actuator mounted on the supply line of nutrient salts, making it possible, in order to improve the quality of control, to be equipped with series-connected the sawtooth signal, comparison, amplification and conversion units, the output of the latter is connected to the actuator installed on the supply line of nutrient salts, and the output of the microorganism growth rate determination unit through the matching unit of the control unit, and an inductor installed on the supply line of the test salts.

Description

The invention relates to medical technology, in particular to microbiology, in particular to devices controlling the processes of fermentation and cultivation of microorganisms,

A known system for automatically controlling the cultivation process of microorganisms, comprising a pH sensor coupled via a pH regulator to an actuator installed on the ammonia water supply line, and an actuator installed on the nutrient salt supply line of the HP.

The disadvantage of this system is that it does not provide the required quality control of the supply of nutrient salts to the fermenter.

Closest to the proposed system of automatic control of the cultivation process of microorganisms, containing a pH sensor connected via a pH regulator with an actuator installed on the supply line of ammnachnoy water, and serially connected blocks for determining the parameters of self-oscillations of pH and determining the growth rate of microorganisms and an actuator mounted on the supply line of nutrient salts, while the input of the unit for determining the parameters of self-oscillations pH is connected to the regulator pH, and the output of the microorganism growth rate determination unit. Through an additional regulator is connected with the actuator installed on the supply line zj.

The disadvantage of this system is that it does not provide the required quality of control, since it is necessary to use an actuator with a high accuracy class. .

The purpose of the invention is to improve the quality of control.

The goal is achieved by those. That a system for automatically controlling the cultivation process of microorganisms, comprising a pH sensor coupled via a pH regulator to; The mechanism installed on the ammonia water supply line and with successively connected blocks for determining parameters of pH self-oscillations and determining the growth rate of microorganisms and the actuator installed at the supply line of nutrient salts is equipped with series-connected blocks of sawtooth signal, comparing, amplifying and converting, the output of the latter is connected to the actuator installed on the supply line of nutrient salts, and the output of the unit for determining the growth rate by matching the microorganisms which is associated with block comparison unit, wherein for feeding nutrient salts choke line set.

FIG. I depicts a schematic diagram of the automatic yjipaBleky process of microbial cultivation; in fig. 2 - plots on the principle of obtaining a control signal with a variable pulse duration.

The system works as follows.

Wort, air, ammonia water and nutritional salts are fed into the fermenter I with a constant pressure. The control system contains a pH sensor 2 connected via a pH regulator 3 to an actuator 4, installed on the ammonia water supply line, an actuator 5 and a choke 6 installed on the nutrient salt supply line, a unit 7 for determining self-oscillating pH parameters, b Approximately 8 determinations of the growth rate of microorganisms, block 9 of matching, and sequence: but connected by a block 10 of a different signal1, block 1 I of a comparison, block 12 of a gain, and block I3 of a conversion. The signal from pH sensor 2 cul-. In the fermenter 1, a pH regulator 3 is supplied, which compares the desired pH value of the culture medium with the current one and forms a sialy; 1) of the actuator 4, which controls the supply of ammonia to the farmer. Depending on the insensitivity of the regulator, self-oscillations of the pH of the culture medium with a constant amplitude appear in the system. At the same time, the rate of decrease in the pH of cultures of a strong medium is proportional to the rate of pocta of the microorganism | Ma, and the rate of increase in the pH of the culture medium is proportional to the difference between the rates of supply of ammonia water and the growth of the microorganism.

In block 7, the determination of parameters (self-oscillations of the pH of the culture medium) determines the parameters of the self-oscillations: the continuation of the increase in pH and decrease in the pH of the culture medium. In block 8, the parameters of self-oscillations determine the growth rate of microorganisms. where, as a driver signal, it is fed to the input of the comparator unit II, which generates a control signal for the actuator 5 installed on the supply line of nutrient salts. The sawtooth signal is formed in the sawtooth signal 10, which is also fed to the input of the comparator unit 11. A pulsed signal is obtained at the output, the pulse duration of which varies depending on the output signal of the matching unit, i.e., the growth rate of the microorganism M0. ) a change in the 01f1is signal proportional to the growth rate of microorganisms The dotted line 1 @ shows the supported signal). It is given by the input of block 1 of the comparison, where it is compared with the sawtooth signal, which is fed from block 10 of the sawtooth signal (rpaijMK b). The resultant signal with a variable pulse duration (plot c) from the output of the comparison unit 1I is fed to block 12 amplified, where it is amplified in power and then fed to conversion unit 13, the signal is converted into a signal suitable for controlling the actuator 3, installed on the supply line nutritional salts. Thus, when the control pulses arrive, the actuator is fully opened and the feed salts enter the fermenter. In the intervals between pulses, the actuator is closed and the supply of nutrient salts is stopped. Since the differential pressure (y on the choke installed on the feed salt supply line is constant, the flow rate, when the actuator opens, is determined by the capacity of the choke and maintains with the required accuracy.

Thus, the automated control system for the cultivation of microorganisms ensures precise control of the supply of feed salts to the fermenter depending on the growth rate of the microorganisms and ensures high quality control.

An analysis of the advantages of this control system was carried out by experimental studies of it on a semi-industrial fermenter. The results obtained were compared with the prototype, taken as the base object, and with a typical technological process, where the supply of nutrient salts is carried out manually using measuring tanks and a pump.

The analysis showed that the proposed control system provides high quality control of the supply of nutrient salts, facilitates the work of apparatchiks and increases the productivity of the department of preparation of nutrient salts of yeast production. The use of the control system improves the quality indicators by an average of 5.0-6.5% compared to the base object.

Claims (1)

AUTOMATIC CONTROL SYSTEM OF CULTIVATION OF MICRO-ORGANISMS, containing a pH sensor connected via a pH regulator with an actuator installed ίγ'-Λ * 'located on the ammonia water supply line, and with series-connected units for determining the pH parameters of self-oscillations and determining the growth rate of microorganisms, and installed on the feed line of nutrient salts, characterized in that, in order to improve the quality of control, it is equipped with serially connected sawtooth blocks th signal comparison, amplification and conversion yield of the latter is connected to an actuator mounted on the feed line of nutrient salts, and an output unit determining the speed of growth of microorganisms by neniya acceptance unit, and salts of tatelnyh connected to a unit comparable to the feed line, a choke is installed. Air Nutrient Salts Ammonia Water Suspension fie. 1>