SU1291862A1 - Method of determining papaverin and pachycaprin in case of their mutual presence in solution - Google Patents

Abstract

. The invention relates to medicine, in particular to methods for analyzing mixtures of salts of physiologically active organic bases, intended for pharmacies and control and analytical laboratories in analyzing and controlling the content of papaverine and pahicardin in the joint presence. The purpose of the invention is to improve the accuracy of determination of papaverine and pahikarpin with their joint presence. For this purpose, in the determination of papaverine, the method of direct potentiometry with preliminary acidification of the analyzed solution to a pH of 2.1-2.7 is used, using an ion-selective electrode sensitive to organic cations. The total content of papaverine and pahicarpin is determined by potentiometric titration using sodium tetraphenylborate as the titrant, using an ion-selective electrode sensitive to organic cations. The content of pahicarpin is equal to the difference between the total content of papaverine and pahicarpin and the content of papaverine. 3- tab. (L with 1C with 00 Od N5

Description

The invention relates to medicine, in particular to methods for analyzing mixtures of salts of physiologically active organic bases, and can be used in pharmacies and control and analytical laboratories for analyzing and controlling the contents of papaverine and pachikarpin when they are present together.

The purpose of the invention is to improve the accuracy of determination of papaverine and pachycarpine when they are present together.

To determine papaverine, the method of direct potentiometry is used by preliminary acidification of the analyzed solution to a pH of 2.1-2.7 using an ion-selective electrode sensitive to organic cations, and the total content of papaverine and pachycarpine is determined by potentiometric titration using tetraphenyl- sodium borate as a titrant and using an organic cation-sensitive ion-selective electrode, the content of pachycarpine being determined by the difference between papaverine and pachycarpine and papaverine

The method is carried out as follows.

Analyzing 0.2 g of the mixture (precise weighting) of the composition: Papa Verin hydrochloride (1ShGH) 0.1 g; Pahicarpine hydroiodide (PCHI) O, 1 g, is placed in a 50 ml volumetric flask, dissolved in a small volume of water, and then brought to the mark with water. A precisely selected volume of the solution (20 ml) is transferred to a 50 ml beaker and the pH of the solution is adjusted to 2.4 with a concentrated solution of HC 1 using a magnetic stirrer, using a glass H-selective electrode to control the pH. The potential of the indicator ion-selective electrode is then measured, the membrane of which consists of PVC (25 wt.X), dibutyl phthalate (70 wt.%, And trioctoxybenzenesulfonic acid (5 wt.%) Relative to the chloro-silver-based comparison electrode on the universal ionomer. the calibration graph for the logarithm of the concentration of papaverine, determine the concentration of papaverine corresponding to the removal of the dose, and calculate the content of papaverine in the mixture

0

five

0

with

After measuring the potential, a solution of 10 M sodium tetraphenylborate is added to the analyzed solution in small portions from a burette with a division of 0.1 ml. Then the solution is held after each added portion for 10–15 s and the corresponding potential value is removed. At the point of equivalence, a solution of sodium tetraphenyl borate is added in portions of 0.1 ml. According to the obtained results, the titration curve is constructed and the volume of sodium tetraphenylborate solution is found corresponding to the equivalence point. Based on the data obtained, calculate the total content of papaverine and pahykarpina. The content of pachycarpine is determined by the difference between the total content of papaverine and pahycarpine and the content of papaverine.

 The statistical processing was carried out according to the results of five analyzes presented in Table 1.

Table 1

0.1003 ± iO, 00240, 2.001 ± to, 0010

0.0998 ± 0.0024

Example 1. Determination of PGH and PCHI in a mixture: PGH 0.1 g; PHGI 0.05 g

Analysis of the mixture for papaverine was carried out as described using an ion-selective electrode, the membrane i qrpporo consists of PVC (25 wt.%), Oxygen bromonaphthalene (70 wt.%) And trioctoxybenzenesulfonic acid (5 wt.%).

. The sum of PGGH and PCHI is also determined in the same way as described, but in a separately selected portion of the initial solution (20 ml). The results of the analysis are presented in table 2.

table 2

12918624

Example 2. Determination of PPGH. The analysis of the mixture is carried out anapogically and PPGI in the mixture: PPGH 0.1 g; The example 1 with three different values is 0.1 g. no x pH. The results of the analysis are presented in table 3.

Table 3

2-, 7 0.102010.0020 0.2000010.0011 0.0982 + 0.0020 2.4 0.1005 ± 0.0024 0.2000010.0007 0.0998 ± 0.0024 2, G 0.0980+ 0,0022 0,200110,0010 0,102110,0022

Claims (1)

An invention of using an ion-selective electrode characterized in that. The method of determining papaverine and the aim of increasing the accuracy of the ophthalmicus when they are combined when each component in the mixture is combined, in solution, including op-determination of papaverine, analysis and determination of papaverine by directing the mixture acidified to a pH of 2.1 Potentiometric determination and 2.7, and the amount of pahikarpin opresumma papaverine and pahikarpin is divided by the difference between the total potentiometric titration with plant content of papaverine and pahicarpivorum sodium tetraphenylborate from is-and content of papaverine.