SU1427299A1 - Method of quantitative analysis of nonachlasin - Google Patents

Abstract

The invention relates to analytical chemistry, in particular, the quantitative determination of nona-aliasine used in medicine. Ana-; tease is carried out by amperimetric titration of the sample with Na-salt of a 12-molybdenum phosphoric heteropolyacid. This reagent allows an increase in sensitivity up to 5 µg / ml and an accuracy of up to 0.925 against 3.08% with a detection time of 15 minutes, 1 tab.

Description

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The invention relates to a method for the determination of nonahlazine - 2-chloro-, 4-diaaebicyclo (4.3.0) -nonayl-4-propionyl phenothiazine dihydrochloride- which can be found in -. change in the work of analytical laboratories

The purpose of the invention is to increase the sensitivity with a pH of 1.8-2.0. The resulting solution is placed in an electrochemical / cell with an electrode system, the voltage is set to 0.10 V and titrated with a solution of sodium salt of a 12-molybdenum phosphoric heteropoly acid (510 mol / l) in portions of 0.50 MP.

accuracy and accuracy, 0.10 ml each is added. Fixation: Example 1, 0.0300 g of nonahlazine (exact weight corresponding to the content of the drug in the tablet) is dissolved in 10.0 ml of distilled water, 5.0 ml (l mol / l) of sodium sulfate electrolyte solution is added, 10, 0 ml of a buffer polarographic solution (a mixture of phosphoric, acetic and boric acids) with pH 1.8-2.0 "The solution is transferred into an electrochemical cell with an electrode system (the indicator is an end graphite electrode with a working surface diameter of 5 mm and a speed of rotation 660 rpm; reference electrode - saturated caloma a half-element, set the voltage to + 0.10 V and fix the value of zero current. Add a titrant (5-10. mol / l sodium salt solution of 12-molybdenum-phosphoric heteropoly acid) to the electrochemical cell in 0.50 portions. ml, within the equimiminum point, the detectable end of the nonahlazine solution 10 mo

the valence of the titrant is added to 0.10 ml. The fixation of the diffusion value is a definable minimum of 5.0 µg / ml, a constant current (Jj;) 60 s after the addition of the titrant. Amperometric titration is completed after a sharp increase in Jj- | The volume of titanium, according to the time of the method for the determination of nano-lasine in colic 15

Claims (1)

Formula inventions titrated, determined graphically on the amperometric titration curve, EXAMPLE 2: Weighted and triturated:, ™ A tablet of nonahlazine is dissolved in 10.0 ml of distilled water, 5.0 ml (10 mol / L) of sodium sulfate solution and 10.0 ml of a buffered polarographic solution are added. ranks jj. 60 seconds after the addition of the titrant. The volume of titrant that is titrated is determined graphically on the amperometric titration curve. Other components of the tab: kids do not affect the course of analysis, The table shows the results of a comparative analysis of the measurement accuracy of the proposed method and prototype (p 7; P 0.95), The accuracy of the proposed method in comparison with the prototype is more than 3 times higher (standard deviation is 0.925 and 3.08%, respectively) -. The scatter of data or the reproducibility of the method is much less than that of the prototype. The method of quantitative determination of nonahalines by the method of ammerometric titration is characterized by high sensitivity, the absence of outliers, Minimum determined concentration of nonahlazine solution 10 mol / l a detectable minimum of 5.0 µg / ml, a detectable minimum of 5.0 µg / ml, The time of carrying out the method of quantitative determination of nonahlazine is 15 min. Invention Formula The method of quantitative determination of nonahalase, characterized in that, in order to increase the sensitivity and accuracy of determination, the analyzed sample is subjected to amperometric titration with sodium salt of 12-molybdenum phosphoric heteropoly acid. 103.27 98.03 95.42 104.53 99.33 98.03 94.00 and 98.44 S ± 3.08 S, ± 1.16 A ± 2.842 and 98.44 + 2.84 X 100.28 S ± 0.925 S, ° 1 0.349 A + 0.855 and 100.28 0.855