SU1237979A1 - Method of quantitative determining of activation of lymphoid cells - Google Patents

Abstract

The invention makes it possible to increase the sensitivity of determining the activation of lymphocytes. To do this, a previously obtained bioassay is placed in a non-luminescentric cuvette with optical quartz bottom glass, located directly above the photocathode of the photomultiplier, which is thermostatically controlled at 38 ° C. A sample of lymphocytes is placed in a cuvette and after 2-3 minutes the intensity of luminescence is recorded. A solution of timostimulin or another inducer is added, 5–10 minutes after the introduction of the immunostimulator, the chemoluminescence is determined and the activation of lymphocytes is evaluated by the number of pulses per unit-time. Table I C 9 th 00; o

Description

The invention relates to medicine, namely to immunology, and concerns methods for detecting and objectively recording the state of lymphocyte activation, and can be used in the diagnosis of diseases accompanied by changes in the body's immunological reactivity.

The goal of the Reteni is to increase the sensitivity of the method.

The method is carried out as follows.

 I 1 ml-gvpryyy of ovarian blood is applied to f A mixture of ficoll and Vorrohr, & (Р-1,077), Cent tubes ;; The EHUs are poured at 1000 rpm for 30 minutes, the BpwrpoiwTbi settle to the bottom of the tube, the lymphocytes remain in the interface. Lymphocytes are harvested, washed twice and counted (from 1 ml of blood with a normal content of lymphocytes

but it manages to get about 10 cle.

current, which is resuspended in 1 ml of Hanks solution). Thus obtained bioassay is placed in a cuvette of non-luminescent glass with a bottom

5 of optical quartz, positioned. Immediately above the photocathode of the PM, thermostatically controlled at 38 ° G. After the reference measurements, the luminescence value of the cell (background) and

 About biobelling cuvettes. A sample of lymphocytes in a volume of 0.7-1.0 ml (10 mm phocytes) is placed in a cuvette and after 2-3 minutes the intensity of luminescence is recorded by the method described above.

 5 Then add 0.7 ml of timostimulin or other inducer solution and after 5-10 minutes the luminescence of the stimulated lymphocytes is again recorded,

20 Chem Iluminides immunocompetent cells 5 min after the stimulator is shown in the table,

Human lymphocytes, Levamisole 0.1 µg / mp

and-

0.15 mg / ml I, 0 mg / ml

9 10

0.1 µg / ml 6 1.0 mg / ml 6

1, About mg / ml 6

Claims (1)

Invention Formula The method of quantitative determination of the activation of lymphoid cells by: adding immunostimulators to lymphocyte lymphocyte suspensions followed by taking into account the results obtained, tVNIIIPI 251 82 9 10 123 27 27 204 74 36 33 41 In order to increase the sensitivity of the method, chemiluminescence is determined within 5-10 minutes after the introduction of the immunostimulator and the activation of lymphocytes is evaluated by the number of pulses per unit time.