SU1125199A1 - Method for detecting lead in blood - Google Patents

Abstract

METHOD FOR DETERMINING LEAD IN THE BLOOD, based on the fluorescent determination of protoporphyrin, which is based on the fact that, in order to improve the accuracy, speed up and optimize the method, a blood sample, fluorescent standard and substrate are irradiated with emitting light in the range of 580-670. Using a spectrophotometer, the fluorescence intensity of the sample, the fluorescent standard and the substrate, and the lead concentration is determined by the formula. with „-if IfEb; -. where - lead concentration - fluorescence intensity of the sample IP - fluorescence intensity § subglome Jll Ig - fluorescence intensity {// standard fluorophore, B - constant

Description

The invention relates to toxicology and can be used to determine lead in the blood and control its concentration during treatment.

A known method of determining the ion of lead in the blood [lj.

However, the method does not allow quantification.

A known method for determining lead in the blood by the content of coproporphyrin in the urine [2].

However, the known method is laborious, time-consuming, requires more reagents and gives an approximate estimate.

The purpose of the invention is improving accuracy, accelerating and simplifying the method.

This goal is achieved by the fact that, according to the method for determining lead in blood by a fluorescent method _20, a blood sample, a fluorescent standard and a substrate are irradiated with exciting light in the range of 580-670 nm, and the fluorescence intensity of a sample · ₂ jca, fluorescence standard and the substrate is measured using a spectrophotometer and determine the concentration of the pig formula where C _C8

Jo about ΣίΖΣϋ

I -1 _and ' ^x s and

- lead concentration; sample intensity;

- the intensity of the substrate;

- Intensity of a standard fluorophore;

- constant (0.8 μg / 100 ml)., carry out the following .40 fluorescence fluorescence ^ torus fluorescence when exposed to exciting sample in the range of 580-670 nm on the test sample, fluorescent standard and substrate. The concentration of lead in the blood is calculated by the formulas where C _c6 et g = in Σ $ _Σϋ ^С C8 ^b I -I 'с η

- lead concentration}

- the fluorescence intensity of the sample;

- fluorescence intensity of the substrate;

Ι _β is the fluorescence intensity of a standard fluorophore;

B is a constant (0.8 μg / 100 ml). Example 1. Patient A. has been working with lead for 8 years. A blood sample (0.25 ml) is placed on the spectrophotometer carriage next to the fluorescent standard.

In relative units, the fluorescence intensities of the sample, substrate, and standard when exposed to stimulating light are 10, respectively; 0.5 and 0.65. According to the formula, the concentration of lead in the blood is C = 50 μg / 100 ml.

CR

Example 3. Patient B. working with lead for 1 year. A blood sample (0.25 megapixels) is placed on the spectrophotometer carriage next to the fluorescent standard. In relative units, the fluorescence intensities when exposed to exciting light are respectively 6.5 for the sample, substrate and standard; 0.5 and 0.65. ! According to the formula, the concentration of blood pigs is found:

With _St. = 32 μg / 100 ml.

The proposed method allows. after calibrating the device, quickly determine the concentration of lead in the blood with high accuracy. In this case, an insignificant amount of patient blood (0.25 ml) is required, and the use of a serial spectrophotometer allows examinations to low-skilled personnel.

IN

A way at once.

1 drop of blood (0.25 ml) of the examined patient is placed on the carriage of the spectrophotometer. On the other nest of the same carriage, the same amount of the fluorescent standard of rhodamine B dissolved in ethylene glycol (concentration 8 mg / mp) is placed, the quantum yield of which is 0.65. Detect sequentially

Claims (1)

A METHOD FOR DETERMINING LEAD IN BLOOD, based on a fluorescent determination of protoporphyrin, characterized in that, in order to increase the accuracy, speed up and simplify the method, a blood sample, a fluorescent standard and a substrate are irradiated with exciting light in the range of 580-670 nm, recorded using a spectrophotometer, the fluorescence intensity of the sample, fluorescence standard and substrate and determine the concentration of lead by the formula Ι1Ξΐη._ where - lead concentration? - the fluorescence intensity of the sample? - fluorescence intensity, substrate? - fluorescence intensity of a standard fluorophore *, - constant (0.8 μg / 100 mp). 1 1125199