SU1227662A1 - Device for taking samples from apparatus - Google Patents

Description

The invention relates to a technique of sterile sampling of ionogenic media for biochemical and microbiological analysis from apparatus operating under aseptic conditions, mainly fermentors for the submerged cultivation of microorganisms, cells and viruses, and can be used in drug production processes and vaccines in enterprises. x biological, microbiological and medical industry. In addition, it can be used for sterile introduction of various additives, for example, components of nutrient media in fermentation devices and other sealed vessels.

The aim of the invention is to prevent pathogenic microflora from entering the environment from the sampling port and, thus, to ensure the safety of servicing the device.

The drawing shows the proposed device, a longitudinal section.

The device for taking samples from the apparatus consists of a syringe, including a cylindrical body 1, divided by a partition 2 into a sterilization chamber 3 and a chamber 4 inside which there is a guide, made in the form of a piston 5. In the center of the guide there is an opening 6, which is on top piston 5 communicates with channel 7 of rod 8, which is sealed tightly on the piston. The rod 8 is equipped with a fitting 9, to which the receiving capacitance 10 is hermetically connected, made of heat-resistant and biologically neutral material, for example, in the form of a pear made of silicone rubber. From below, the opening 6 is connected to the channel 1 of the needle 12, which is made of a capillary tube fixed at one end on the piston 5 and connected by a channel. 1b with channel 7. The opposite end of the needle is plugged and pointed. At the base of the plug, on the side of the needle 12, there is a hole 13, located in the sealing sleeve 4, which is pressed by the nut 15 with a hole through the center through which the needle 12 passes.

The chamber 3 is internally equipped with a heating element 16 in the form of a metal spiral and is filled with a biologically neutral easily meltable

and fast freezing, paraffin, paraffin.

Outside the housing 1 there is a projection on which a cap

nut 17. The lower part of the body (sterilization chamber 3) enters inside another sterilization chamber 18, filled (by analogy with chamber 3) with paraffin, and fits tightly with

through the sealing gasket 19. The sterilization chamber 18 is permanently mounted on the sampling port 20 of the apparatus 21 for the submerged cultivation of microorganisms, cells and viruses. Inside the chamber 18 is provided with a heating element 22. At the bottom of the center there is an elastic gasket 23, preloaded with a nut 24 with a hole

centrally for the passage of the needle 12. Outside the sterilization chamber 18, there is a cooling jacket 25 with an inlet 26 and an outlet 27 inlets.

To limit the movement of the piston 5 upwards, a cap nut 28 is mounted on the housing 1.

The device works as follows.

I PreBartel1) But about sterilized

in an autoclave, a device, the sterilization chamber 3 of which is filled with paraffin frozen after sterilization during the sampling process,

„„, -,

The help of the cap nut 17 through the gasket 19 fits tightly with the sterilization chamber 18, also filled with solidified paraffin (the initial position of the stem 8 is the upper extreme one). Then, paraffin is heat-sterilized at the points of attachment of housing 1 to chamber 18, for which heating elements 16 and 22 are turned on for a certain time (5-7 minutes) and paraffin is heated to 125-130 ° C. After sterilization is completed, paraffin is cooled in sterilization chambers 3 and 18 to a temperature below

sterilization temperature (65-70 ° C) When paraffin reaches the specified temperature, the rod 8 is moved to the lower end position. Avoid blockage with liquid paraffin

the channel 11 of the needle 12 at the moment of its passage through the molten paraffin by pressing the pear 10 is carried out} and the air is blown

11 through the hole 13 until the needle gets inside the apparatus 21. After that, the pear opens and due to elastic forces it restores its previous volume. As a result of the dilution tank 10 created inside the suction tank 10, the liquid (or gas) from the apparatus is sucked inside the tank. The rod 8 returns to its original position. The heating elements 16 and 22 are switched on and during 5-7 minutes sterilization of the joining places of chambers 3 and 18, the end of needle 12, nuts 15 and 24, and sealing elements 14 and 23 is performed. Upon completion of sterilization with water supply. cooling jacket 25 paraffin is hardened in chambers 3

and 8, and then the decoupling of the housing 1 and the sterilization chamber 18. Selected from the apparatus 21 into the receiving tank 10 of the proAa together with the syringe is transferred to a specially equipped sterile room for biochemical and microbiological examination.

The use of the proposed device for sampling pathogenic media for biological and microbiological analysis will allow, in comparison with known devices, to reduce the loss of the fermentation medium as a result of excluding its acidification from extraneous microflora; improve the sanitary and hygienic conditions of the staff.

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Compiled by G. Bogachev Editor L. Veselovska Tehred G. Gerber Proofreader S. Shekmar

..... M ™ .in.in-. ., - "-" - "-..- -.- .- .--" in- - - --- -Order 2263/27 Circulation 490Subscribed.

VNIIPI USSR State Committee

for inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab., 4/5

Production and printing company, Uzhgorod, st. Project, 4

Claims (1)

DEVICE FOR SAMPLING FROM APPARATUS, mainly fermenters, containing a syringe with a receiving container mounted on the sampling pipe of the fermenter having an elastic gasket pressed by a nut, characterized in that, in order to prevent pathogenic microflora from the sampling pipe from entering the environment and, thus to ensure the safety of servicing the device, the syringe body and the sampling pipe are equipped with sterilization chambers with heaters that communicate with each other when they are docked and filled with paraffin, while the sterilization chamber of the sampling pipe has a cooling jacket.