CN220176774U - Mycoplasma culture medium preparation device capable of preventing pollution - Google Patents
Mycoplasma culture medium preparation device capable of preventing pollution Download PDFInfo
- Publication number
- CN220176774U CN220176774U CN202321667900.2U CN202321667900U CN220176774U CN 220176774 U CN220176774 U CN 220176774U CN 202321667900 U CN202321667900 U CN 202321667900U CN 220176774 U CN220176774 U CN 220176774U
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- culture medium
- cavity
- test tube
- medium preparation
- connecting plate
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- 239000001963 growth medium Substances 0.000 title claims abstract description 41
- 241000204031 Mycoplasma Species 0.000 title claims abstract description 20
- 238000011177 media preparation Methods 0.000 title claims abstract description 18
- 238000007789 sealing Methods 0.000 claims abstract description 36
- 238000002156 mixing Methods 0.000 claims abstract description 31
- 229920001971 elastomer Polymers 0.000 claims abstract description 29
- 238000004659 sterilization and disinfection Methods 0.000 claims description 29
- 239000007788 liquid Substances 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 13
- 238000004321 preservation Methods 0.000 claims description 9
- 241000204003 Mycoplasmatales Species 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 3
- 210000002966 serum Anatomy 0.000 abstract description 18
- 238000002360 preparation method Methods 0.000 abstract description 10
- 230000001954 sterilising effect Effects 0.000 abstract description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 14
- 238000000034 method Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 241001138504 Mycoplasma bovis Species 0.000 description 1
- 206010028470 Mycoplasma infections Diseases 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920005549 butyl rubber Polymers 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a mycoplasma culture medium preparation device capable of preventing pollution, which comprises a box body, wherein a mixing cavity, a sterilizing cavity and a steam generation cavity are sequentially arranged in the box body from top to bottom, a charging port is arranged at the upper end of a sealing cover, a rubber plug is movably inserted in the charging port, a sealing plug is movably inserted at the bottom of a connecting plate, and an electric telescopic rod is arranged on the connecting plate. Adopt above-mentioned technical scheme to make a mycoplasma culture medium preparation facilities that prevents pollution, through inserting the rubber buffer in the charge door, sealed the charge door, when adding the serum to mixing the intracavity simultaneously, can directly stab the needle tubing that contains the serum into the rubber buffer and carry out the joining of serum, need not to pull out the rubber buffer and reinsert, further prevent to lead to the fact the pollution to the culture medium preparation in the preparation process.
Description
Technical Field
The utility model relates to the technical field of culture medium preparation, in particular to a mycoplasma culture medium preparation device capable of preventing pollution.
Background
Mycoplasma is a kind of minimum prokaryotic cell type microorganism which has no cell wall, high polymorphism and can pass through a filter and can be cultured and proliferated by using an artificial culture medium, mycoplasma culture means that mycoplasma is inoculated into a culture medium, the growth condition of the mycoplasma is observed to judge whether mycoplasma infection exists, the culture medium of mycoplasma is different from the culture medium of common bacteria, because the nutrition requirement of mycoplasma is higher than that of common bacteria, 10% -20% of human or animal serum is required to be added into the culture medium of mycoplasma besides basic nutrient substances to provide cholesterol and yeast required by mycoplasma, therefore, serum is required to be additionally added into the common culture medium preparation and mixed, for example, an utility model with an authorized bulletin number of CN215050281U discloses a preparation device for a chromogenic culture medium of mycoplasma bovis, a split charging test tube bracket, an ultraviolet disinfection lamp and a quantitative valve are arranged to place split charging test tubes into a test tube groove, the split charging is sterilized, and then the quantitative valve is opened, the prepared culture medium is dripped into the test tube through split charging and split charging test tubes, so that the prepared culture medium is effectively polluted, and the quality of the culture medium is easily influenced when the test tube is in a sterile state, the test tube is required to be in a test tube, the test tube is easy to be in contact with an external state, but the preparation is easy to be in a sterile state when the test tube is required to be in a test tube, and the test tube is in an external state, the test tube is required to be in an external state, and the test tube is easy to be exposed to be in a sterile port, and the test tube is easy to be polluted, and the external port is easy to be in the preparation port and the external port is easy to be polluted. For this purpose, we propose a mycoplasma culture medium preparation device that prevents contamination.
Disclosure of Invention
In order to solve the problems, the utility model provides the following technical scheme: the utility model provides a prevent mycoplasma culture medium preparation facilities of pollution, includes the box, the inside mixing chamber, disinfection chamber and the steam generation chamber of having set gradually from last to down of box, the box upper end is connected with the closing cap through the cylinder subassembly, be provided with stirring subassembly on the closing cap, the closing cap upper end is provided with the charge door, the activity is pegged graft in the charge door has the rubber buffer, be provided with the branch tubulation between mixing chamber and the disinfection chamber, be provided with the valve on the branch tubulation, the movable joint in the disinfection chamber has test tube holder, the disinfection intracavity is connected with the connecting plate through apart from the regulating part, the movable plug that has pegged graft in connecting plate bottom, be provided with electric telescopic handle on the connecting plate, be provided with the pipe between steam generation chamber and the disinfection chamber, the disinfection chamber side is provided with the shower nozzle.
Preferably, a heat preservation cavity is arranged on the side wall of the mixing cavity, and a liquid inlet pipe and a liquid outlet pipe are respectively arranged on two sides of the heat preservation cavity.
Preferably, the test tube seat comprises a bottom plate and a sleeve, the bottom plate is movably clamped at the bottom of the disinfection cavity, the upper end of the bottom plate is fixedly connected with the sleeve, and the sleeve and the split charging tube are coaxially arranged.
Preferably, an elastic pad is fixedly connected to the side wall of the sleeve.
Preferably, the sealing plug is fixedly connected with a rubber pad, the bottom end of the connecting plate is fixedly connected with a contact pin, and the contact pin is inserted into the rubber pad.
The utility model has the advantages and beneficial effects that: the utility model provides a mycoplasma culture medium preparation device capable of preventing pollution, which is characterized in that a rubber plug is inserted into a feed inlet to seal the feed inlet, so that external bacteria are prevented from entering a preparation cavity through the feed inlet, pollution to a culture medium is reduced, meanwhile, when serum is added into a mixing cavity, a needle tube containing the serum can be directly penetrated into the rubber plug to add the serum, after the completion of the addition, the needle tube is directly taken out, the rubber plug is still sealed to the feed inlet, the rubber plug is not required to be pulled out and reinserted, pollution to a culture medium preparation in the preparation process is further prevented, high-temperature steam can be introduced into a disinfection cavity through the cooperation of a steam generation cavity and a guide pipe, a test tube and a culture medium reagent in the test tube are sterilized, after the sterilization is completed, a sealing plug can be inserted into the test tube through the cooperation of a distance adjusting piece and an electric telescopic rod, and then the test tube is taken out, and pollution to the culture medium in the process of the test tube is further prevented.
Drawings
In order to more clearly illustrate the embodiments of the utility model or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the description below are only some embodiments of the utility model, and that other drawings can be obtained according to these drawings without inventive faculty for a person skilled in the art.
FIG. 1 is a front cross-sectional view of the present utility model;
FIG. 2 is a schematic perspective view of the case of the present utility model;
fig. 3 is an enlarged schematic view at a in fig. 1.
In the figure: the device comprises a box body 1, a mixing cavity 2, a sterilizing cavity 3, a steam generating cavity 4, a cylinder assembly 5, a sealing cover 6, a stirring assembly 7, a charging port 8, a rubber plug 9, a heat preservation cavity 10, a liquid inlet pipe 11, a liquid outlet pipe 12, a valve 13, a sub-pipe 14, a test tube seat 15, a bottom plate 151, a sleeve 152, an elastic pad 153, a distance adjusting piece 16, a connecting plate 17, a sealing plug 18, an electric telescopic rod 19, a rubber pad 20, a plug rod 21, a guide pipe 22 and a spray head 23.
Detailed Description
The following describes the embodiments of the present utility model further with reference to the drawings and examples. The following examples are only for more clearly illustrating the technical aspects of the present utility model, and are not intended to limit the scope of the present utility model.
As shown in figures 1-3, the utility model relates to a mycoplasma culture medium preparation device for preventing pollution, which comprises a box body 1, wherein a mixing cavity 2, a sterilizing cavity 3 and a steam generating cavity 4 are sequentially arranged in the box body 1 from top to bottom, common culture medium reagents can be mixed and stirred in the mixing cavity 2 to prepare common culture medium reagents, a split-charging test tube is arranged in the sterilizing cavity 3, after the culture medium reagents of mycoplasma are split-charged in the split-charging test tube, the test tube and the culture medium can be sterilized, a steam generator and a water source are arranged in the steam generating cavity 4, high-temperature steam can be introduced into the sterilizing cavity 3 through the steam generating cavity 4, and the test tube is sterilized by the high-temperature steam;
the side wall of the mixing cavity 2 is provided with a heat preservation cavity 10, two sides of the heat preservation cavity 10 are respectively provided with a liquid inlet pipe 11 and a liquid outlet pipe 12, the liquid inlet pipe 11 and the liquid outlet pipe 12 are externally connected with a preheating liquid, a liquid pump and the like, the preheating liquid with a certain temperature can be introduced into the heat preservation cavity 10, the mixing cavity 2 is subjected to heat preservation and heating, the requirement of reagent preparation is met, and in addition, a temperature sensor can be arranged in the mixing cavity 2, so that the temperature in the mixing cavity 2 can be conveniently controlled;
the upper end of the box body 1 is connected with a sealing cover 6 through an air cylinder assembly 5, the sealing cover 6 is provided with a stirring assembly 7, the stirring assembly 7 can be a matching piece of a stirring shaft, a stirring blade and a motor, the stirring assembly 7 extends into the mixing cavity 2 and can mix and stir reagents in the mixing cavity 2, the air cylinder assembly 5 can drive the sealing cover 6 to ascend or descend, when the sealing cover 6 ascends under the action of the air cylinder assembly 5, the stirring assembly 7 can be taken out of the mixing cavity 2 at the same time, the stirring assembly 7 is convenient to clean and disinfect, pollution to the next reagent preparation is reduced, when the sealing cover 6 descends under the action of the air cylinder assembly 5, the sealing cover 6 can seal the mixing cavity 2, and sealing assemblies such as sealing rings and the like can be arranged between the sealing cover 6 and the mixing cavity 2, so that the sealing performance of the mixing cavity 2 is ensured;
the upper end of the sealing cover 6 is provided with a feed inlet 8, the feed inlet 8 is movably inserted with a rubber plug 9, because the nutrition requirement of a mycoplasma culture medium is higher than that of a common culture medium, serum and the like are required to be added into a common culture medium preparation, the growth requirement of the mycoplasma is met, the serum can be added into the mixing cavity 2 through the feed inlet 8, the rubber plug 9 can be a butyl rubber plug, when the serum is added, the serum can be sucked into a needle tube, the needle tube pierces the rubber plug 9, the serum can be directly added into the mixing cavity 2 by using the needle tube, the needle tube can be directly pulled out of the rubber plug 9 after the addition, and the rubber plug 9 is not required to be taken out or reinserted into the feed inlet 8 in the addition process of the serum, so that the feed inlet 8 is always kept in a sealed state, and the pollution to the reagent in the mixing cavity 2 is effectively reduced;
a sub-tube 14 is arranged between the mixing cavity 2 and the disinfection cavity 3, a valve 13 is arranged on the sub-tube 14, a test tube seat 15 is movably clamped in the disinfection cavity 3, a hinged door can be arranged on the side surface of the disinfection cavity 3, the disinfection cavity 3 is convenient to open, a sub-packaging test tube is placed on the test tube seat 15, the test tube seat 15 can be pulled out of the disinfection cavity 3, after the preparation of a culture medium is completed, the valve 13 can be opened, the sub-tube 14 is utilized to add the reagent in the mixing cavity 2 into the sub-packaging test tube on the test tube seat 15, no manual intervention is needed, and the pollution is further reduced;
the test tube seat 15 comprises a bottom plate 151 and a sleeve 152, the bottom plate 151 is movably clamped at the bottom of the disinfection cavity 3, the upper end of the bottom plate 151 is fixedly connected with the sleeve 152, the sleeve 152 and the split-tube 14 are coaxially arranged, an elastic pad 153 is fixedly connected to the side wall of the sleeve 152, the split-tube can be placed in the sleeve 152 to limit the split-tube, the elastic pad 153 can be an elastic rubber pad, and the split-tube is protected and limited;
the disinfection cavity 3 is internally connected with a connecting plate 17 through a distance adjusting piece 16, a sealing plug 18 is movably inserted at the bottom of the connecting plate 17, an electric telescopic rod 19 is arranged on the connecting plate 17, the distance adjusting piece 16 can be an electric telescopic rod or an air cylinder assembly, the distance adjusting piece 16 is used for adjusting the sealing plug 18 to the upper part of a split charging test tube, the electric telescopic rod 19 is used for pressing the sealing plug 18 downwards into the split charging test tube, the sealing plug 18 is separated from the connecting plate 17, after the sealing is finished, the disinfection cavity 3 is opened again, the split charging test tube is taken out, and the pollution to reagents in the split charging test tube can be effectively reduced;
the sealing plug 18 is fixedly connected with a rubber pad 20, the bottom end of the connecting plate 17 is fixedly connected with a contact pin 21, the contact pin 21 is inserted into the rubber pad 20, and the sealing plug 18 can be inserted into the bottom end of the connecting plate 17 by utilizing the cooperation of the contact pin 21 and the rubber pad 20, so that the subsequent separation of the sealing plug 18 and the connecting plate 17 is facilitated;
be provided with pipe 22 between steam generation chamber 4 and the disinfection chamber 3, disinfection chamber 3 side is provided with shower nozzle 23, is provided with steam generator in the steam generation chamber 4, is provided with the liquid filling port on the steam generation chamber 4, and the high temperature steam that steam generator produced can be imported to shower nozzle 23 through pipe 22, is imported to disinfection chamber 3 through shower nozzle 23, carries out the high temperature sterilization to split charging test tube and the culture medium reagent after split charging.
When the device is used, raw materials of common culture medium reagents are added into the mixing cavity 2, the stirring assembly 7 is utilized for stirring and mixing, the sealing cover 6 can seal the mixing cavity 2 when the action of the air cylinder assembly 5 is lowered, the serum can be sucked into the needle tube when the serum is added, the needle tube is used for puncturing the rubber plug 9, the serum can be directly added into the mixing cavity 2 by the needle tube, after the addition is finished, the needle tube can be directly pulled out of the rubber plug 9, the rubber plug 9 is not required to be taken out or reinserted into the feed inlet 8 in the process of adding the serum, the feed inlet 8 always maintains a sealing state, the pollution to the reagent in the mixing cavity 2 is effectively reduced, the split charging test tube is placed on the test tube seat 15, after the preparation of the culture medium is finished, the valve 13 can be opened, the reagent in the mixing cavity 2 is added into the split charging test tube seat 15 by the split charging tube, manual intervention is not needed, the pollution is further reduced, high-temperature steam generated by the steam generator can be directly led into the spray head 23 by the guide tube 22, the spray head 23 is led into the disinfection cavity 3, the split charging process is not required to be taken out of the rubber plug 9, the split charging process is carried out by the electric sealing plug 18, the split charging process is further, the split charging test tube is separated by the electric sealing plug 18 and the sealing plug 18 is separated by the electric sealing plug 18, the split charging process is further, the split charging test tube is separated by the sealing plug 18, the split sealing plug 18 is separated into the split test tube, and the split charging test tube is separated into the test tube, and the test tube is finally, and the test tube is completely separated by the split sealing test tube is separated, and the test tube is 18, the split after the test tube is 18, and the split.
The foregoing description of the preferred embodiments of the utility model is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the utility model.
Claims (5)
1. The utility model provides a prevent mycoplasma culture medium preparation facilities of pollution, includes box (1), its characterized in that, box (1) inside has set gradually hybrid chamber (2), disinfection chamber (3) and steam generation chamber (4) from last extremely down, box (1) upper end is connected with closing cap (6) through cylinder subassembly (5), be provided with stirring subassembly (7) on closing cap (6), closing cap (6) upper end is provided with charge door (8), movable grafting has rubber buffer (9) in charge door (8), be provided with branch pipe (14) between hybrid chamber (2) and disinfection chamber (3), be provided with valve (13) on branch pipe (14), disinfection chamber (3) internal activity joint has test tube holder (15), be connected with connecting plate (17) through distance adjusting part (16) in disinfection chamber (3), connecting plate (17) bottom activity grafting has sealing plug (18), be provided with electric telescopic handle (19) on connecting plate (17), be provided with between steam generation chamber (4) and disinfection chamber (3) and shower nozzle (23) side.
2. The mycoplasma culture medium preparation device for preventing pollution according to claim 1, wherein a heat preservation cavity (10) is arranged on the side wall of the mixing cavity (2), and a liquid inlet pipe (11) and a liquid outlet pipe (12) are respectively arranged on two sides of the heat preservation cavity (10).
3. The mycoplasma culture medium preparation device for preventing pollution according to claim 1, wherein the test tube holder (15) comprises a bottom plate (151) and a sleeve (152), the bottom plate (151) is movably clamped at the bottom of the disinfection cavity (3), the upper end of the bottom plate (151) is fixedly connected with the sleeve (152), and the sleeve (152) and the split charging tube (14) are coaxially arranged.
4. A mycoplasma culture medium preparing apparatus according to claim 3, wherein an elastic pad (153) is fixedly connected to the side wall of the sleeve (152).
5. The mycoplasma culture medium preparation device for preventing pollution according to claim 1, wherein a rubber pad (20) is fixedly connected to the sealing plug (18), a contact pin (21) is fixedly connected to the bottom end of the connecting plate (17), and the contact pin (21) is inserted into the rubber pad (20).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321667900.2U CN220176774U (en) | 2023-06-29 | 2023-06-29 | Mycoplasma culture medium preparation device capable of preventing pollution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321667900.2U CN220176774U (en) | 2023-06-29 | 2023-06-29 | Mycoplasma culture medium preparation device capable of preventing pollution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220176774U true CN220176774U (en) | 2023-12-15 |
Family
ID=89113727
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321667900.2U Active CN220176774U (en) | 2023-06-29 | 2023-06-29 | Mycoplasma culture medium preparation device capable of preventing pollution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220176774U (en) |
-
2023
- 2023-06-29 CN CN202321667900.2U patent/CN220176774U/en active Active
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