SU1212416A1 - Method of extracting albumin from blood plasma - Google Patents

Description

The invention relates to the technology of obtaining protein preparations and can be used in the medical industry.

The aim of the invention is to increase the yield of the target product.

Example. Vials with dry plasma of long periods of hr nei (up to 4 years) are opened daily on a fractionation day. Dry pore; okg, 6 star1 iva1s | t and pinned

The last of the last impurity mi protein

of nature.

Sodium Caprylate is loaded into the measuring device.

at the rate of 30 ml of 10% solution for 1 l

centrifugate (2 + 3).

In mernik load 1 n. solution

hydrochloric acid at the rate of 70 ml per 1 l

centrifugal.

In mernik load 1 n. caustic solution

soda at the rate of 45 ml per 1 liter of centrifuge in the jacketed reactor, -k. him with non-Q fugata. discontinuous mixing | 1 (roll-out centro- pugate (2 - + - 3) is loaded into the reacted g) 1 stylized into ду for the half, turn on the stirrer and serve I in homogeneous. m, sy and. cool the head of the reactor with water with a mixture temperature of up to 0 ° G Na, .. 9v g of powder from 35 to 50 ° C. After applying the heat dry plasma. 6epyf 1000 ml of pyrogen-free water into the jacket of the reactor from the mernik occasion. To the plasma cooled to 0 ° C, 15% solution of sodium hydroxide is added to the reactor with a 10% solution. Then from the measuring device cooled to (- 15) - (- 20 °) 53% ethanol at the rate of 180 ml per 1000 ml of plasma. The final alcohol concentration in the mixture is 8%. The temperature of the mixture in the process of adding alcohol is gradually reduced from 0 ° to-3 ° C by the end of the precipitation. At the end of the addition of alcohol, the mixture is left for 3 to 6 hours.

20

at (-3 ± 1) ° С. In these conditions,

start serving 1 n. hydrochloric acid solution until the pH of the mixture reaches 3.3. After the pH of the mixture is set (3.3), and the temperature has reached 14-15 ° C, the mixture is kept for 20 minutes. After 20 minutes, a solution of caustic soda is fed to the reactor from the measuring device until a pH of 4.6 is reached. For complete maturation of the precipitate, the mixture is kept up to the beginning. The precipitate is precipitated by 1-fraction of plasma proteins, 25 centrifuged at pH 4.6 for containing mainly fibrinogen. Dropping-20 min. Then centrifuged at 17 ° C.

The precipitate is separated by centrifugation. The centrifugate is filtered on a suction filter. The centrifugate is measured and loaded into the reaction. The filtrate, having a temperature of 18 ° C, is collected in a reactor, in the jacket of which

The precipitation of the fraction (2 + 3) is carried out by circulating the cooled solution. By measuring slowly (no more than 12 liters per hour) 30, 96% ethyl alcohol is added from the addition of alcohol to the centrifugate at a rate of 220 ml per 1 liter of filtrate. Alcohol of a couple of 610 ml on 1 l. Alcohol before adding is cooled to (- 15) - (-20) ° C. In this case, the temperature of the mixture in the reactor is gradually reduced to (-5) - (- 6) ° C. After the precipitation is complete, the mixture is stirred for at least 2 hours.

The precipitate was separated by centrifugation. The centrifugate (2 + 3) is measured and loaded into a reactor equipped with

the mixer and fed into the jacket of the reactor. Then it is centrifuged, the centrifugate is water with a temperature of 35-50 ° C. with the production waste, and the residue of the fraction contains electrophoretic pure

The third stage is the most responsible albumin. The crude precipitate of albumin by the plant stage of albumin purification when it is thrown, is frozen at a time, “quenched — released from dry plasma. Dry and remove the rf and p globum powder to prepare the solution of the desired concentration, hemoglobin traces, cleavage, sterile filter, pour into albumin complex compounds and remove vials.

cooled to a temperature of (- 15) - (-20) ° C, turn on the reactor stirrer and mix thoroughly, to avoid foaming, the filtrate loaded at the previous stage. In order to precipitate all albumin, chilled 96% alcohol is added to the filtrate. The temperature of the mixture at the end of the precipitation is reduced to (-8) - (-10) ° C and left to mature for 10 hours.

the cooled solution circulates. 96% ethyl alcohol is loaded into the measuring device at the rate of 220 ml per 1 l of filtrate. Alcohol

Then it is centrifuged, the centrifugal is with the production waste, and the precipitate is cooled to (–15) - (-20) ° C, the reactor mixer is turned on and thoroughly mixed, avoiding foaming, the filtrate loaded in the previous stage. In order to precipitate all albumin, chilled 96% alcohol is added to the filtrate. The temperature of the mixture at the end of the precipitation is reduced to (-8) - (-10) ° C and left to mature for 10 hours.

Claims (1)

METHOD FOR ISOLATING ALBUMINE FROM BLOOD PLASMA by sequential precipitation of fibrinogen and globulins followed by precipitation from the obtained albumin supernatant with ethyl alcohol, characterized in that, in order to increase the yield of the target product, the globulin precipitation stage is carried out by adding a stabilizer and heating to 14–15 ° С at pH 3.3, followed by adjusting the pH to 4.6.