SU1185239A1 - Method of preparing erythrocytes preserved at low temperature for transfusion - Google Patents
Method of preparing erythrocytes preserved at low temperature for transfusion Download PDFInfo
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- SU1185239A1 SU1185239A1 SU833618359A SU3618359A SU1185239A1 SU 1185239 A1 SU1185239 A1 SU 1185239A1 SU 833618359 A SU833618359 A SU 833618359A SU 3618359 A SU3618359 A SU 3618359A SU 1185239 A1 SU1185239 A1 SU 1185239A1
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- USSR - Soviet Union
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- preserved
- transfusion
- low temperature
- erythrocytes
- preparing
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Изобретение относитс к медицине, а именно к низкотемпературному консервированию эритроцитов человека, а может найти применение на станци х переливани крови и банках крови. ,The invention relates to medicine, namely to the low-temperature preservation of human erythrocytes, and may be used at blood transfusion stations and blood banks. ,
Цель изобретени - снижение гемолиза эритроцитов в цроцессе размораживани .The purpose of the invention is to reduce the hemolysis of erythrocytes during the thawing process.
Пример. Эритроциты, консервированные в алюминиевом гофрированном JQ контейнере конструкции ЦНИИГПК (14-100-300 мм) в объеме 250 мл под защитой 30% глицерина и 35% 1,2-пропандиода из жидкого азота перенесли в пенопластовый чехол с толщиной стенок 40 мм и зазором между стенками чехла и контейнера 2 мм и выдерживали в нем 16 мин до достижени температуры - (4,8°С/мин.), Затем контейнер перенесли в воду с температурой +45 °С и вьщержали в ней 75 с (100 С/мин) до полного оттаивани материала.Example. Erythrocytes preserved in a JQ aluminum corrugated container of the TsNIIGPK construction (14-100-300 mm) in a volume of 250 ml under the protection of 30% glycerol and 35% of 1,2-propane diode from liquid nitrogen were transferred to a foam case with a wall thickness of 40 mm and a gap between the walls of the cover and the container were 2 mm and kept there for 16 minutes until the temperature reached - (4.8 ° C / min.). Then the container was transferred to water with a temperature of +45 ° C and held there for 75 seconds (100 C / min) until complete thawing of the material.
Сохранность эритроцитов определ ли по уровню гемолиза в размороженных эритроцитах. Гемолиз составл л: при консервировании с глицерином 352 мг%,с 1,2-пропандиолом 174мг%. Дл контрол параллельно проводили одноэтапное .размораживание эритроцитов путем погружени контейнеров в вод ную баню с температурой . При этом гемолиз составл л: при контсервировании с глицерином 631 мг%, р 1,2-пропандиолом 286 Red blood cell integrity was determined by the level of hemolysis in thawed red blood cells. Hemolysis was: when preserved with glycerol, 352 mg%, with 1,2-propanediol, 174 mg%. For controlling in parallel, a one-stage erythrocyte defrosting was performed by immersing the containers in a temperature water bath. At the same time, hemolysis was as follows: when conserved with glycerol, 631 mg%, p 1,2-propanediol 286
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU833618359A SU1185239A1 (en) | 1983-07-11 | 1983-07-11 | Method of preparing erythrocytes preserved at low temperature for transfusion |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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SU833618359A SU1185239A1 (en) | 1983-07-11 | 1983-07-11 | Method of preparing erythrocytes preserved at low temperature for transfusion |
Publications (1)
Publication Number | Publication Date |
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SU1185239A1 true SU1185239A1 (en) | 1985-10-15 |
Family
ID=21073173
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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SU833618359A SU1185239A1 (en) | 1983-07-11 | 1983-07-11 | Method of preparing erythrocytes preserved at low temperature for transfusion |
Country Status (1)
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SU (1) | SU1185239A1 (en) |
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1983
- 1983-07-11 SU SU833618359A patent/SU1185239A1/en active
Non-Patent Citations (1)
Title |
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Методическое письмо. Методы . долгосрочного хранени в замороженном состо нии эритроцитов, предназначенных дл трансфузии, М., МЗ СССР, 1980. * |
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