CN213085950U

CN213085950U - Umbilical cord mesenchymal stem cell's recovery device

Info

Publication number: CN213085950U
Application number: CN202021408704.XU
Authority: CN
Inventors: 樊晋宇; 张诗薇; 池金鹏; 赵亚琪; 程雪; 申昕炜
Original assignee: Shandong Tianchuan Precision Medical Technology Co Ltd
Current assignee: Shandong Tianchuan Precision Medical Technology Co Ltd
Priority date: 2020-07-17
Filing date: 2020-07-17
Publication date: 2021-04-30
Anticipated expiration: 2030-07-17

Abstract

The utility model belongs to the technical field of the medical instrument technique and specifically relates to a recovery device of umbilical cord mesenchymal stem cells. Including cord blood cryopreserved bag and centrifugation bag, cord blood cryopreserved bag include first cavity and second cavity, first cavity and second cavity between be equipped with the governing valve, cord blood cryopreserved bag on be equipped with the injection port, the injection port be equipped with a plurality of, the centrifugation bag including transplanting the bag and shifting the bag, the transplantation bag on be equipped with and shift the pipe, the pipe that shifts on be equipped with the stop valve, the pipe end that shifts be equipped with the puncture ware, the puncture ware set up on the injection port of cord blood cryopreserved bag, transplantation bag and transfer bag between be equipped with communicating pipe, communicating pipe on be equipped with the stop valve, transplantation bag and transfer bag on all be equipped with the puncture port.

Description

Umbilical cord mesenchymal stem cell's recovery device

Technical Field

The utility model belongs to the technical field of the medical instrument technique and specifically relates to a recovery device of umbilical cord mesenchymal stem cells.

Background

Brief introduction: in recent years, medical research shows that cord blood contains very abundant Hematopoietic Stem Cells (HSCs) and can restore the human hematopoietic and immune systems. Umbilical cord blood hematopoietic stem cell transplantation is an effective method for treating diseases such as leukemia, lymphopathy, aplastic anemia and the like. Therefore, umbilical cord blood has become an important source of hematopoietic stem cells, has been widely used in clinic, and is a valuable human biological resource. So the number of people who choose to cryopreserve cord blood is increasing. However, whether cord blood transplantation is successful or not is also very important in the resuscitation process, in addition to the concentration and cryopreservation steps. The freezing and thawing of cord blood cells follows the principle of slow freezing and fast thawing, and the freezing and thawing method comprises the following basic steps: cell freezing solution preparation → 0 ℃ ice water bath → -80 ℃ temperature control refrigerator → -196 ℃ liquid nitrogen preservation. When the cord blood cell sample is recovered, the cord blood cell sample can be melted in a water bath at 37 ℃ for 2 minutes. Because DMSO is toxic to cells and its high osmotic pressure property can cause a large number of cells to lose activity and even die, cell resuscitants require centrifugation to remove DMSO from suspension. Therefore, when the cell sample is just thawed, a proper amount of protective agent needs to be added to dilute the solution and adjust osmotic pressure, so as to achieve the purpose of protecting the cells.

Quote: the utility model discloses a recovery device of umbilical cord blood umbilical cord mesenchymal stem cells of publication number CN207912954U, including recovery liquid bag, transplant bag and transfer bag, use recovery bag to the injection recovery liquid in umbilical cord blood cryopreserving bag in to umbilical cord blood recovery process, transfer the umbilical cord blood of resuscitating to the transplant bag again, transfer to the transplant bag after centrifugation, transfer to the transplant bag after the centrifugation, this device is connected in umbilical cord blood recovery process and is transferred the process and all used injection tube and puncture ware, easily increase umbilical cord blood and pollute the risk, and put transplant the area and transfer bag simultaneously in centrifuge centrifugation, easily cause the problem of bad connection between the bag, cause the pollution of umbilical cord blood. (ii) a

The novel conception is as follows: the utility model provides a design umbilical cord mesenchymal stem cells's recovery device, reduces the connecting tube between each bag body, reduces the pollution risk of cord blood.

SUMMERY OF THE UTILITY MODEL

For solving the resuscitation problem of the hematopoietic stem cells, the utility model provides a resuscitation device of umbilical cord mesenchymal stem cells.

The utility model provides a technical scheme that its technical problem adopted is: the utility model provides a recovery device of umbilical cord mesenchymal stem cell, freezes including the umbilical cord blood and deposits bag and centrifugal bag, the umbilical cord blood freeze and deposit the bag and include first cavity and second cavity, first cavity and second cavity between be equipped with the governing valve, the umbilical cord blood freeze and deposit and be equipped with the injection port on the bag, the injection port be equipped with a plurality of, the centrifugal bag including transplanting the bag and shifting the bag, the transplantation bag on be equipped with the transfer pipe, the transfer pipe on be equipped with the stop valve, transfer pipe end be equipped with the puncture ware, the puncture ware set up on the injection port that the umbilical cord blood freezes and deposits the bag, transplantation bag and transfer be equipped with communicating pipe between the bag, communicating pipe on be equipped with the stop valve, transplantation bag and transfer bag on all be equipped with the puncture port.

As optimization, first cavity be the resuscitation liquid chamber, the second cavity freeze and deposit the chamber for the cord blood, first cavity and second cavity between be equipped with the connecting pipe, the governing valve set up on communicating pipe, the governing valve include valve member and seal box, the seal box top be equipped with the opening, the seal box lower part set up in first cavity, seal box bottom set up in first cavity outside, seal box outer wall and the bag body sealing connection of first cavity, the seal box both sides be equipped with the through-hole, the connecting pipe pass two through-hole settings, connecting pipe one end set up in first cavity, the connecting pipe other end set up in the second cavity, the valve member set up in the opening. The connection part of the communicating pipe and the sealing box is connected in a sealing way.

Preferably, the number of the injection ports is two, the two injection ports are respectively a first injection port and a second injection port, the first injection port and the second injection port are respectively arranged on the first cavity and the second cavity, and the puncture outfit is arranged on the second injection port.

As optimization, the stop valve include fixed block and locking part, the fixed block be the U type, the fixed block on be equipped with the screw, locking part include locking screw and locking plate, locking screw set up in the screw, the locking plate set up in locking screw terminal.

Preferably, the centrifugal bag is made of polyvinyl chloride.

The whole beneficial effect of this scheme is: the cord blood cryopreservation bag is set into two cavities, the resuscitation solution is injected into the first cavity, and then the resuscitation solution is directly transferred into the second cavity through the transfer tube to resuscitate the cord blood, so that the injection time in the resuscitation solution injection process can be reduced, and the pollution risk is reduced; and will shift the bag and transplant the bag and set up on same bag body, and shift the connecting line setting between bag and the transplantation bag and at the internal portion of bag, can reduce the chance that connecting line and external world contacted, reduce the infection risk.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is the structure schematic diagram of the regulating valve of the present invention.

Fig. 3 is a schematic view of the section structure of the regulating valve of the present invention.

Figure 4 is the utility model discloses a stop valve structure sketch map.

The umbilical cord blood cryopreservation bag comprises an umbilical cord blood cryopreservation bag body, a centrifugal bag body, a first cavity body, a second cavity body, a regulating valve, a first transplantation bag body, a second cavity body, a regulating valve, a second cavity body, a third cavity body, a fourth.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate the position or positional relationship based on the position or positional relationship shown in the drawings, or the position or positional relationship which is usually placed when the product of the present invention is used, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.

In the embodiment shown in fig. 1, the umbilical cord mesenchymal stem cell recovery device comprises an umbilical cord blood cryopreservation bag 1 and a centrifugal bag 2, the cord blood freezing and storing bag 1 comprises a first cavity 3 and a second cavity 4, an adjusting valve 5 is arranged between the first cavity 3 and the second cavity 4, the cord blood freezing and storing bag 1 is provided with a plurality of injection ports, the centrifugal bag 2 comprises a transplanting bag 6 and a transferring bag 7, a transfer tube 8 is arranged on the transplantation bag 6, a stop valve 9 is arranged on the transfer tube 8, a puncture outfit 10 is arranged at the tail end of the transfer tube 8, the puncture outfit 10 is arranged on the injection port of the cord blood freezing bag 1, a communicating pipe 11 is arranged between the transplanting bag 6 and the transferring bag 7, the communicating pipe 11 is provided with a stop valve 9, and the transplanting bag 6 and the transferring bag 7 are both provided with puncture ports 12.

First cavity 3 be the resuscitation liquid chamber, second cavity 4 for the chamber is deposited to cord blood freezes, governing valve 5 set up on communicating pipe 11, governing valve 5 include valve member 13 and seal box 14, first cavity 3 and second cavity 4 between be equipped with the connecting pipe, seal box 14 top be equipped with the opening, seal box 14 lower part set up in first cavity 3, seal box 14 bottom set up in first cavity 3 outsidely, seal box 14 outer wall and the bag body sealing connection of first cavity 3, seal box 14 both sides be equipped with the through-hole, the connecting pipe pass two through-hole settings, connecting pipe one end set up in first cavity 3, the connecting pipe other end set up in second cavity 4, valve member 13 set up in the opening.

The two injection ports are respectively a first injection port 15 and a second injection port 16, the first injection port 15 and the second injection port 16 are respectively arranged on the first cavity 3 and the second cavity 4, and the puncture outfit 10 is arranged on the second injection port 16.

Stop valve 9 include fixed block 17 and locking part, fixed block 17 be the U type, fixed block 17 on be equipped with the screw, locking part include locking screw 18 and locking plate 19, locking screw 18 set up in the screw, locking plate 19 set up in locking screw 18 end.

The device is when specifically using, use the syringe to pour into the first cavity 3 with recovery liquid in, adjust valve member 13, open governing valve 5, make recovery liquid flow into in the cord blood cryopreserving bag 1, then transfer pipe 8 on centrifugal bag 2 is connected through puncture ware 10 and second injection port 16, open stop valve 9, discharge mixed liquid into transplant bag 6, close transplant bag 6 and transfer the stop valve 9 between the bag 7, put into centrifuge with centrifugal bag and centrifuge, after the centrifugation, beat stop valve 9, discharge upper liquid into transfer bag 7 among, transfer on next step.

The above embodiments are only specific cases of the present invention, and the protection scope of the present invention includes but is not limited to the product form and style of the above embodiments, any resuscitating device of umbilical cord mesenchymal stem cells according to the claims of the present invention and any suitable changes or modifications made thereto by those skilled in the art shall fall within the protection scope of the present invention.

Claims

1. The utility model provides a recovery device of umbilical cord mesenchymal stem cell which characterized in that: including cord blood cryopreserved bag and centrifugation bag, cord blood cryopreserved bag include first cavity and second cavity, first cavity and second cavity between be equipped with the governing valve, cord blood cryopreserved bag on be equipped with the injection port, the injection port be equipped with a plurality of, the centrifugation bag including transplanting the bag and shifting the bag, the transplantation bag on be equipped with and shift the pipe, the pipe that shifts on be equipped with the stop valve, the pipe end that shifts be equipped with the puncture ware, the puncture ware set up on the injection port of cord blood cryopreserved bag, transplantation bag and transfer bag between be equipped with communicating pipe, communicating pipe on be equipped with the stop valve, transplantation bag and transfer bag on all be equipped with the puncture port.

2. The resuscitation device for umbilical cord mesenchymal stem cells according to claim 1, wherein: first cavity be the resuscitation liquid chamber, the second cavity freeze and deposit the chamber for the cord blood, first cavity and second cavity between be equipped with the connecting pipe, the governing valve set up on communicating pipe, the governing valve include valve member and seal box, seal box top be equipped with the opening, seal box lower part set up in first cavity, seal box bottom set up in first cavity outside, seal box outer wall and the bag body sealing connection of first cavity, seal box both sides be equipped with the through-hole, the connecting pipe pass two through-hole settings, connecting pipe one end set up in first cavity, the communicating pipe other end set up in the second cavity, the valve member set up in the opening.

3. The resuscitation device for umbilical cord mesenchymal stem cells according to claim 1, wherein: the two injection ports are respectively a first injection port and a second injection port, the first injection port and the second injection port are respectively arranged on the first cavity and the second cavity, and the puncture outfit is arranged on the second injection port.

4. The resuscitation device for umbilical cord mesenchymal stem cells according to claim 1, wherein: the stop valve include fixed block and locking part, the fixed block be the U type, the fixed block on be equipped with the screw, locking part include locking screw and locking plate, locking screw set up in the screw, the locking plate set up in locking screw terminal.

5. The resuscitation device for umbilical cord mesenchymal stem cells according to claim 1, wherein: the centrifugal bag is made of polyvinyl chloride.