SU1172887A1 - Method of biochemical treatment of waste water - Google Patents

Abstract

1. METHOD OF BIOHMASH CLEANING of sewage by microorganisms in the presence of synthetic latex and with stirring, characterized in that, in order to increase the degree of purification, microcultures use cultures of Micrococcus luteus, Phodotonila glutinis, Rseudomonas aerugindsa, or their mixture, as a synthetic, as a synthetic, synthetic, as a synthetic, synthetic, as a synthetic, synthetic synthesis, or as a synthetic synthesis. - divinyl styrene la, tex, which is introduced into the cell suspension. 2. The method according to claim 1, of which it is introduced into the suspension of β-cells at a concentration of 200-800 mg / l.

Description

The invention relates to methods for the biochemical treatment of wastewater and to be used in industrial areas where pure cultures of microorganisms are used for the treatment of wastewater. There is a known method of introducing into the active strain pure cultures of microorganisms possessing a high specific destructive activity. The disadvantage of this method is that bacterial cells in a dispersed state practically do not settle to the bottom of the secondary settler and the entire biomass growth goes with purified water, as a result of which a new contamination of wastewater occurs, as well as process activity, since it is impossible to create a high concentration of microorganisms in the system without recirculation. There is a method for improving the structural and sedimentation properties of activated sludge during biochemical purification by introducing high molecular weight (:: street compounds: polysaccharides, RNA and DNA proteins, as well as synthetic latexes. The disadvantage of this method is with an increase in the oxidative capacity of activated sludge only by strengthening the flakes and improving the sedimentation properties using the activity of spontaneous microflora without the targeted effect of certain specialized strains of microorganisms purpose of biodegradation of organic pollutants The aim of the invention is to increase the degree of purification. The aim is to ensure that the biochemical purification is carried out with stirring by cultures of microorganisms Nicrococcus luteus, Rhodotorula glutinis, Pseudomonas aeruginosa or their mixture in the presence of divinyl sterile latex, which is introduced into suspension, and the suspension is suspended in a suspension. , preferably to a concentration of 200800 mg / l. Artificial bioflocks can be obtained from microorganisms belonging to any genus, but the species attribute determines the specific conditions of the flooding process. Cultivation: density of microbial suspension, concentration of synthetic latex. interaction time of two dispersed systems, sufficient for sensitization, age of the culture of microorganisms. With a high density of the slurry of Got 10-15 g / l on a dry weight basis, flocculation takes place instantly when grinding the suspension and after several hours at a lower density. Instant flocculation can also be achieved for a suspension of microorganisms with a density below critical (the critical density is different in suspensions of various microorganisms) by introducing a flocculin stimulator, for example, a polyvalent Al cation, concentration of 0.050.2 mg / cubic meter of mg of sediment. The culture age does not affect the flocculation process in all microorganisms. Yeast and pseudomonads flocculate better when young cells predominate in suspension, and for microcox culture age does not matter. The flocculation process is equally important for microorganisms belonging to different genera, influenced by the pH of the medium, the ionic strength of the solution, the presence of organic substances. , In an acidic environment, flocculation proceeds faster, but in order to preserve the normal viability of cells, flocculation is best carried out at a pH value that is optimal for the development of microorganisms. Flocculation is more intense with a greater ionic strength of the solution, but it is necessary to observe the physiological capabilities of the cells. Organic matter leads to the stabilization of colloids, therefore flocculation proceeds better in an environment where there is no organic matter. Example I. A suspension of a daily culture of Micrococcus luteus grown on a dense nutrient medium, in a synthetic medium without a source of uterol or in a physiological solution, pH 6-7, is prepared. The density of the suspension is 1.5-2.0 units of distillation. Divinyl styrene latex is added at a concentration of 200 mg / l. Create intense mixing of the liquid. After 24 hours, flocks with a sludge index of 52 cm / g are obtained. With a load of organic matter up to 2000 mg Oj / l COD and flock concentration 2 g / l (in Sukhoi Becyi, after 24 hours, the COD level decreases by 85%.

PRI mme R 2. At the same UI, a suspension of Micrococcus luteus with a density of 2.75-3.0 units-ex sintine (critical density for this culture) is prepared, divinylstyrene latex is added at a concentration of 200-300 mg / l. and shaken or bubbled. During l-S minutes of attack, O et flocculide. Floks silt index of about 60 cm / g. By their oxidizing ability, these flocks do not differ from those obtained by the method given in Example 1.15. Measurement 3. A mixture of three

daily cultures, active strains of destructors of vinyl esters of tlicole: Pseudomonas aeruginosa strain - specific destructor of divinyl esters of glycols, Micrococcus liiteus strain - specific destructor of monovinyl ethers

glycol strain Phodotorxila glutinis - specific destructor 25 2 methyl 1,3 dioxolane (isomer of monovinyl ether of ethylene glycol |, flocculates, Flood association 3 times washed with phosphate buffer (pH 7.2 or physiological growth-30

by thief and transferred to synthetic medium at a concentration of 4 g / l on a dry basis; vinyl ethers of glycols in a concentration are introduced into the medium as a carbon source. j 2 (MPC for effluent 2 mg / l). Wednesday aerated. After 24 hours, the level of COD reduction is determined. It corresponds to 50% of the original COD value. The same decrease; level: COD is observed when oxidizing glycol vinyl ethers

Organic compound

. under similar conditions, the dispersed cells of three cultures of these microorganisms.

Example4. In laboratory aerotanks Dobiem 2l, aeration period 24 h), biological treatment of waste water containing acetaldehyde, vinyl-n-butyl ether, ethyl acetate, ethanol, butanol at concentrations of 100, 100, 700, 1500 and 300 mg / l, respectively, is carried out. The biomass concentration of 3 g / l dry weight of the obsgn options is only active sludge and active sludge + flocks. The content of organic matter in water is carried out by GLC. The table shows the data on the percentage oxidation of organic compounds by microflora in the case of using one active sludge, active sludge with latex and the proposed method.

The use of fused cells provides the following advantages, namely: easy separation of microorganisms from suspensions, the ability to create biomass recirculation of pure cultures of active microorganism tamms in treatment plants, structures, higher flock viability compared to dispersed cells, allowing repeated use of bioflora cleaning for a long period of time (up to 6 months).

Fulfillment of these conditions will significantly increase the efficiency of wastewater treatment when using pure cultures of microorganisms specially adapted to the degradation of highly toxic organic compounds.

Claims (2)

1. WAY OF BIOCHEMICAL WASTE WATER CLEANING by microorganisms in the presence of synthetic latex with stirring, characterized in that, in order to increase the degree of purification, Micrococcus luteus, Phodotorula glutinis, Rseudomonas aeruginosa cultures or a mixture thereof, as synthetic latex are used as microorganisms latex, which is introduced into a suspension of cells. 2. The method according to π.1, wherein the divinyl styrene latex is introduced into the cell suspension at a concentration of 200-800 mg / L.