SU1131901A1 - Strain pseudomonas aeruginosa 1/5 used for purifying waste liquors at fine organic synthesis plants containing nonionogenic surfactants and morpholine and method for biochemical purification of waste liquors - Google Patents

Abstract

1. The Pseudomonas aeruginosa 1/5 strain (collection of the Central Piece Museum ,,, I. :: I industrial microorganisms of the VNIIGENETIK Institute, collection number CMPM B-2557) used for purification of waste waters of fine organic synthesis, containing a mixture of non-ionic surface -active substances and morpholind. 2. A method of biochemical treatment of wastewater from organic compounds, including treatment with fermented sediment under anaerobic conditions and aerobic purification, is of great concern so that, in order to increase the degree of purification of wastewater, fine organic enterprises, simplifying and cheapening the process, aerobic purification is the strain of Pseudomonas aeruginosa 1/5. 3, Method according to p. 1, characterized in that the fermented precipitate is introduced once.

Description

111 The invention relates to the protection of the environment and can be used for the purification of waste waters of fine organic synthesis enterprises. Wastewater containing high concentrations of non-nutrient surface-active substances (nonionic surfactants) and morpholine, in particular, fine organic synthesis wastewater is usually purified by burning, which is economically disadvantageous. The well-known strain Ps, aeruginosa 1 / destroys a mixture of synthetic organic substances (aniline, phenol, nitro benzene, dimethylaniline, o-nitroanisop, p-nitrophenol, p-phenetizin, naphthalene) found in the waste water of the aniline dye industry lj. However, studies have shown that the addition of one of the nonionic surfactants or morpholine leads to the absence of biodegradation, therefore, wastewater treatment is impossible on biological installations and the only method. water disposal is incineration. Closest to the present invention is a method for purifying wastewater from aromatic nitro compounds, including pretreatment of circulating fermented sludge under anaerobic conditions and aerobic purification of water. Water purification from nitro compounds is carried out in three stages, namely, circulating fermented sediment is treated with sewage to sorb nitro compounds, then the precipitate formed after separation of the mixture containing aromatic nitro compounds is pumped to the digester (anaerobic treatment), and the water is transferred to an aerobic biological cleaning in two-stage, aeration tanks. To clean each new batch of water, it is mixed with a new batch of fermented sludge, and the consumption of the fermented sludge is 0.4-0.5 g of dry matter per 1 mg of the recovered aggregate 2J. The disadvantage of this method is the ability to purify only one-type wastewater containing, for example, individual aromatic nitro compounds. In addition, the applied load for the concentration of 2 grams of oil is low — 50 mg / l; the process technology is three-stage and is associated with a high consumption of fermented sludge, which makes purification much more difficult both in equipment and economically. The aim of the invention is to obtain the active destructor stearox-6, OC-20, hydropol-200, polyethylene glycol-35, with an initial concentration of 1550 mg / l each, and morpholine at a concentration of 300 mg / l and using them in the wastewater treatment of fine organic enterprises. synthesis to increase the degree of purification, simplify and reduce the cost of the process. The goal is achieved by the PS strain. acruginosa 1/5, (collections of the Central Museum of Industrial Microorganisms of the VNIIGENETIK Institute, collection number CMPM B-2557) used to purify wastewaters of fine organic synthesis containing a mixture of non-ionic surfactants and morpholine. In addition, according to the method of biochemical treatment of wastewater from organic compounds, which includes the treatment of fermented sediment under anaerobic conditions and aerobic purification, aerobic purification is carried out using Pseudomonas aeruginosa strain 1/5, and the fermented sediment is introduced once. The strain has the following morphological and physiological properties. 1 The cells are small, rod-shaped, with rounded ends, located singly or in pairs, gram-negative, mobile, with a polar flagellum, do not form a dispute. On mi-peptone agar, the colonies are round, 3-4 mm in diameter, flat, translucent in transmitted light, and wavy edge of the colony. The surface of the colony is smooth, moist, glossy, consistency is viscous. It has a blue-green pigment diffusing into the medium. On the moc-peptone broth, the culture causes a uniform turbidity. Culture oksidazopolozhitelna, catalase, hydrolyzes gelatin, not gidrolnzuet starch, has no lecithinase and levansaharoznoy activity, forms hydrogen sulfide and does not produce indole, milk does not change, glucose fermented, aerobe, capable of denitrification, arginine decarboxylated to withstand the concentration of sodium chloride in the medium of less than five%. The optimum growth temperature is 28-30 ° C, a slight increase is observed at 41 ° C. On the Omel Nskogo mineral medium with water-soluble bromothymol blue it forms an acid from glucose, lactose, maltose, mannitol and sucrose and does not use dulcite, arabinose, glycerin, sodium tartrate. The culture absorbs sodium acetate and citrate. The strain destroys up to 6500 mg / l of a mixture of nonionic surfactants (stearsk-6, OS-30, hydropol-200, proxanol, PL-3, polyethylene glycol-35) and 300 mg / l morpholine. An example of the preparation of the strain. The soil taken on the territory of the plant for organic synthesis was introduced into a flask. liquid mineral medium of the following composition, g / l: fiH jNO-j 1.0; K2HP04 0.6-KH2P04 0.4i NACB. 0.5, nonionic surfactants with a total concentration of 6.5 and morpholine - 0.3. . Cultivation was carried out at 30 with shaking on a rocking chair until complete disappearance of the named substances in the medium. The accumulated culture was obtained by sixfold passages of the 5KID mineral medium with increasing concentrations of IPAV and mofolin. The storage culture was scattered on m-peptone agar and pure cultures of bacteria were detected, whose destructive activity was tested on a liquid mineral medium containing a mixture of nonionic surfactants (stearox-6, OC-20, hydropr-200, proxanol, CP-3, Polyethylene glycap-35) with a total concentration of 6.5 g / l.

I

Prototype

Indicators

 g / l

Constant, 0.4-0.5 g of dry matter of fermented sludge per T mg of recovered contaminants

Separate aromatic

nitro compounds - trinitrotoluene or dinitronaphthalene

I

The proposed method

5 g / l

Single, 0.0006 g of dry matter of fermented sludge per 1 mg of seized contaminants

A mixture of nonionic surfactants of infectious structure and heterocyclic xenobiotic - morpholine. morpholine 300 mg / l. A culture capable of destroying these compounds in the indicated concentrations was screened on the beveled surface of MPA and stored under a layer of sterile vaseline oil. An example of wastewater treatment using strain Ps. aeruginosa 1/5. A waste liquid containing stearox-6, OC-20, hydropol-200, polyethylene glycol-35, morpholine with a total concentration of 6.5 g / l is added to a cylindrical container with a volume of 1 l containing adapted fermented sludge (5 g / l). , COD 2000 mg Oj / l processing time 12 hours without aeration. Then purified water is treated with a strain of Ps.aeruginosa 1/5 at a concentration of 10 cells / ml, the processing time of 12 hours. Purified waste water is characterized as follows: - Non-surfactant content - 7 mg / l; morpholous O, which meets the requirements of MPC for supply to biological treatment plants. According to the proposed method, in the anaerobic stage of purification of the pr, the main part of the contaminants are destroyed (at the initial concentration of 6.8 g / l, the destruction occurs by 90-95%, i.e., to residual concentrations - 340680 mg / l). If such water is directed. activated sludge, then cleaning will not occur due to the toxicity of this water to aquatic sludge. The problem can be solved by applying the selected strain PS. aeruginosa 1/5, Comparative data of the proposed method and the prototype are presented in the table.

Continuation table

a few

50 mg / l Virtually absent

Nonionic surfactants

A three-stage process with multiple injection of circulating fermented sludge used as a sorbent for aromatic nitro compounds

.6800 mg / l 99-100%

Two-stage process in a continuous anaerobic aerobic installation with one-time introduction of specially prepared fermented sludge used as a destructor of organic contaminants

Claims (3)

1. Strain Pseudomonas aeruginosa 1/5 (collection of the Central Museum of Industrial Microorganisms of the Institute VNIIGENETIKA, collection number TsMPM V-2557) used for wastewater treatment of fine organic synthesis plants containing a mixture of nonionic surfactants and morpholine. 2. A method for the biochemical treatment of wastewater from organic compounds ₄ , including treatment with fermented sludge under anaerobic conditions and aerobic purification, which can be done in order to increase the degree of wastewater treatment of fine organic synthesis enterprises , simplify and reduce the cost of the process, aerobic treatment is carried out by the strain Pseudomonas aeruginosa 1/5. 3. The method of item p. 1, characterized in that the fermented precipitate is administered once.