SU1145503A1 - Glomus sp.no.7 endomycorrhizal fungus strain increasing yield of agricultural plants - Google Patents

Abstract

Strain endomycorrhizal fungus Glomus sp. No. 7 (the collection of the All-Union Scientific Research Institute of Agricultural Microbiology), the harvest of agricultural plants. (L

Description

 ate

01

about

00

The invention relates to agriculture, namely to the field of microbiological means of crop yields of agricultural plants.

Additional inoculation of cereals, legumes and other crops in vegetation and field trials with an endophyte breeding crop leads to an increase in yield and an improvement in the phosphorus nutrition of plants, mainly on soils poor in mobile phosphorus compounds. lj /.

The known strain Glomus mosseae, which is used to increase the yield of various agricultural crops and improve phosphorus nutrition 2. However, when conducting experiments with the indicated endophyte strain on sod-podzolic and other soils, its effectiveness was not found, the yield per crop was 4,70- 7.50%.

The purpose of the invention is to obtain a new strain of endomycorrhizal fungi, which during inoculation increases grain yield, by improving the phosphorus nutrition of agricultural plants on poor sperm-podzolic soils and when: potash fertilizer.

The goal is achieved using the strain of the fungus Glomus sp. 7

Strain Glomus sp. No. 7 is derived from the mycotrophic roots of the landowner that grew on well-cultivated soddy-weakly podzolic soil in the Leningrad Region.

The sections of the surface-sterilized roots of the earth are introduced into sterile sod-podzolic soil with a small content of mobile phosphorus, into which the surface-sterilized seeds of barley, oats, or cereals are sown. After various periods of cultivation of these plants (from 1-3 months for cereals and 6 months for cereals), strongly mycotrophic roots of experimental plants with structures characteristic of endomycorrhizal fungi, in particular hyphae and vesicles, are obtained.

Endomycorrhagous fungus has the following characteristics.

The intracellular hyphae located inside the root of the mycelium are thicker than 1 µm in diameter, do not form loops, intracellular rings or tangles, and parallel hyphae have H-junctions. A plurality of consecutively arranged round or oval shaped vesicles with a size of 24-120 µm are observed. The presence of a pear-shaped or rounded spore attached to the roots was noted with a funnel-shaped attachment to the accompanying hypha of yellow-brown color.

Spores are formed in the soil and can be removed from it. They are brown, round-oval in shape, smooth, glossy, the walls are thick, brittle, mostly single-layer, some have two-layer, the upper layer is transparent membrane-like, the inner one is thick, brittle, light brown. As the wall ages, the spores darken and penetrate the pores. Spore sizes range from 182 to 207 microns. The accompanying hypha is inflated and has a funnel-shaped form at the attachment site, its diameter is 24–25 µm, the width of the thin end of the hypha is 3–8 µm. With aging, the hyphal spore is lost. The content of the spores is grainy.

This type of endophyte forms sporocarpous fruit bodies in the pow. They are small, measuring 274-350 microns, each containing 20 spores with a diameter of 80- 99 microns, pear-shaped, light brown in color with a slightly swollen concomitant hyphae. The content of the dispute is streaky. Sporocarpes are like a bunch of grapes. The spores are not fully enmeshed in hyphae.

This endophyte forms mycorrhiza with earth, barley and oats.

On artificial nutrient media is not growing. Supported on mycorrhized roots.

The spore type is close to the vacuolized yellow spores of the Mosse, Bowen system.

According to the systematics Gerdeman, Trappe 2j / mushroom belongs to the genus Gloraus.

Example 1. In a vegetation experiment, vessels with a capacity of 3 kg are filled for 2/3 of an absolutely dry unsterile vulcanized sod-weakly podzolic light loamy soil deposit, characterized by a humus content of 3%, total nitrogen 0.18%

3

PyOf (according to Kirsanov), 2.3 mg / OO g soil, pH 6.6.

Then inoculum is introduced into the vessels in the form of the spity-containing endophyte Glomus sp. № 7 of the mixture of mycotrophic roots with soil. After mixing with inoculation materials, the soil is seeded with surface-sterilized oat seeds of the Khadmerslebener AG variety. Watering the soil is carried out up to 60% of full capacity. Under the same conditions, the experiment was carried out with the culture of Glomus mosseae.

As a control, the same vessels are used, but without inoculum of the nip material. The result of the experiment is given in table. one.

As the table shows. 1, inoculating seeds of oats with the proposed strain provides an increase in the yield of oats grain by 56.9%, which is almost 5 times more than the increase from inoculation by the prototype strain with an increase in phosphorus removal by 51.4%, which exceeds

55034

this figure compared with the prototype strain more than 10 times.

Example 2. Experience set by

5 is similar to the scheme as described in example 1, but using cultivated arable land trap, characterized by a humus content of 3.5%, total nitrogen 0.19%, PiOtff (according to Kirsanov)

to 74.0 mg / 100 g of soil, pH 6.0. The experiment was conducted on the background of the introduction of nitrogen-potassium and nitrogen-phosphorus-potassium fertilizers at the rate of 0.1 g of N, P and K per 1 kg of soil. Experimental results

15 are given in table. 2

As the table shows. 2, inoculum Seed n oats proposed strain. provides (as against full (NPK) fertilizers, and against W)

20 fertilizers) a greater yield increase than inoculation with the prototype strain.

The use of the Glomus sp, ff 7 strain allows an increase of

25 harvests from 10 to 50%.

The effect of inoculation of endomycorrhizal fungi on grain yield

oats and phosphorus removal

1.16 1.30 1.82 0.40

Table 1

1.79 1.88

5.00 51.40 2.71 0.56

The effect of inoculation of endomics with oryznymi fungi against the background of various fertilizers on the yield of grain – oats VON VariantsCrop yield of grains F Without inoculation21.92 Glomus mosseae23,56 Glomus sp. 724.23 -, Without inoculation22,13 Glomus mosseae23,18 NPK - Glomus sp. 7 25.49 NSy.l1 1.62

Table 2 g / soeeda yield yield,% 7.50 10.60 4.70 15.20

Claims (1)

Strain endomycorrhizal fungus S1otiz sp. No. 7 (collection of the All-Union Scientific Research Institute of Agricultural Microbiology), which increases the yield of agricultural plants. 5 and 1145503 1 1145503 2