SU1130348A1 - Method of obtaining saponin processing hydrolipidemic activity - Google Patents

Abstract

THE METHOD OF GETTING SAPONIN, POSSESSING DEFICIENCY ACTIVITY, by extracting Ladiginla bucharica from Bokhara Ladiginia with alcohols followed by thickening of the extract, diluting it with water and extracting with N-butanol, in order to increase the activity of the target program, in order to increase the activity of the target product, with the aim of increasing the activity of the target; ethanol at a water ratio of 1: 7–9, the extract is concentrated to a volume of 12–15% of the original, then diluted with water, acidified, washed, the washed extract is evaporated, dissolved in ethanol, and precipitated with a mixture of petroleum ether - acetone their ratio of 1: 1.

Description

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00 11 The invention relates to methods for producing biologically active prod- ucts from vegetable raw materials, in particular, to a method for obtaining saponin from Ladakhinia bucharica Lipsky (Ladiginia bucharica Lipsky), palm family (um belliferae), with hypolipidemic activity. There is a known method for producing saponin, which possesses the same hypolipidemic activity, from Bukhara logyginia by extraction with alcohol, followed by concentration of the extract, diluting it with water and extraction with H-butanol Cl. However, the known method does not allow to obtain the target product with a sufficient degree of activity. The purpose of the invention is to increase the reactivity of the target product. This goal is achieved according to the method of obtaining saponin with lipid-lowering. activity, by extraction of the Ladakhinia by Bukhara Ladiginia bucharica. with alcohols, followed by concentration of the extract by diluting it with water and extraction with N-butanol, in which the roots are extracted with 75-85% ethanol at a water ratio of 1: 7-9, the cry-extract is extracted to volume 12- 15% of the original, then diluted with water.

Target product

Ladapin Saponin

Reducing the amount of cholesterol in the blood,%

, 1 month 2 months, I 3

months 8 is acidified, washed, the washed extract is evaporated, dissolved in ethanol and precipitated with a mixture of petroleum ether - acetone at their ratio of 1: 1. For example, 50 kg of dried and ground handwashing roots are extracted with 80% alcohol. The hydromodule 1: 3, the extract is concentrated to a volume of 60 liters, diluted with water to 100 liters, acidified with 20% sulfuric acid to pH and the saponins are extracted with N-butanol 7 times 30 liters each time. Butanol extraction is washed with 40 liters of water until neutral and evaporated to dryness, the dry residue is dissolved by heating in 20 l of ethanol and saponins precipitated with 100 l of a mixture of ether — acetone 1: 1, the precipitated precipitate is separated on a suction filter, washed with 15 l of acetone and dried. Output 5 kg or 10 by weight sf. Dedicated saponin has lipid-lowering activity. Data on the activity of the target product are presented in the table. From the presented data, it follows that the proposed method allows to obtain a target product, having high lipid-lowering activity.

Claims (1)

METHOD FOR OBTAINING HYPOLIPIDEMIC SAPONIN ACTIVITY by extraction of rooks ** from Bukhara Ladiginia bucharica with alcohols, followed by thickening of the extract, diluting it with water and extracting with N-butanol, characterized in that, to increase the activity of the target product, the roots are 75% extra ethanol with a water module of 1: 7-9, the extract is concentrated to a volume of 12-I5Z from the original, then diluted with water, acidified, washed, the washed extract is evaporated, dissolved in ethanol and precipitated with a mixture of petroleum ether - acetone 1: 1 ratio.