SU924051A1 - Process for producing ecdysteron - Google Patents

Description

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The invention relates to methods for producing biologically active substances, in particular to a method for producing ecdysterone, used to regulate the growth of silkworms.

Ecdysones are known to influence the development of insects and are widespread in the plant world Γΐ 3 -.

There is also known a method of producing the insect hormone ecdysterone from the ’· plant roots of AcNugal® and CycNi1a, including extraction of crushed vegetable raw materials with alcohol, evaporation of the extract, dilution with water, treatment with ether or chloroform, extraction with ethyl acetate, evaporation, eluting the residue c. aluminum oxide with chloroform-methanol mixture (9: 1) followed by recrystallization of the target product from acetone (2}.

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The disadvantage of this method is a very low yield of the target product (0.005% by weight of raw materials).

The purpose of the invention is to expand the resource base and increase the yield of ecdysterone.

This goal is achieved by the fact that as a source of raw materials used above-ground parts of plants of the genus 5x1epe Stssh. Seeds of carnations.

Suitable, for example, are the species of the Great Patriotic Family by M. Rod., & Pepe 5aH £ oPa.

The yield of the target product is 0.28-0.54% by weight of raw materials.

, 5 Example 1. 55 kg of crushed leaves, stems and inflorescences Smolevka mixed pour 220 liters of ethanol. After infusion overnight, the extract is drained. The ethanol extraction is repeated 3 more times.

at intervals of 8-10 hours, spending on

80 liters of ethanol. The combined alcoholic extract is concentrated to 3 liters and mixed with 3 liters of water, filtered and washed 921 * 051

Wash, shake H times with ethyl ether, and then extract 20 L of ethyl acetate and evaporate to a small volume. As it cools, a precipitate forms, which is separated, dried and washed with ethyl ether [23. The sediment has a weight of 0.6 kg.

The precipitate is placed in a column with aluminum oxide and ecdysterone is washed out with a mixture of chloroform-ethanol (9: 1) ~

30 l. The weight of the residue from the evaporated Eluates is 360 g. After recrystallization from dry acetone and drying, the product yield, i.e. ecdysterone, is 297 g or 0.5 * »$. from air-dry raw materials.

Example Ϊ. 5 kg of the homogenate of the broad leaved pulp is poured with 20 liters of ethanol and after 24 hours of infusion, the extract is drained.

The extraction is repeated three more times in 7 l with an interval of 8-10 hours. The combined extracts are concentrated in vacuo to 0.5 l, mixed with water and filtered. After ethanol was distilled off, the aqueous layer was extracted 4 times with chloroform. Then ecdysterone is extracted from the residue with 5 l of ethyl acetate, evaporated, dried and washed with 0.2 l of ether. The precipitate (35 g) is placed in a column with alumina and ecdysterone is eluted with a mixture of chloroform-ethanol (9: 1) and 6 l. The weight of the dry residue is 22 g. After recrystallization from ethyl acetate and then from acetate, C is obtained, 6 g of ecdysterone C ^ H ₄₄ O ₇ .

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Claims (1)

Claim The method of obtaining ecdysterone, 10 including extraction of crushed vegetable raw materials with alcohol, evaporation of the extract, dilution with water, treatment with ether or chloroform, extraction with ethyl acetate, evaporation, $ 1 elution of the residue from aluminum oxide with a mixture of chloroform-methanol followed by recrystallization of the target product from acetone, which is different from me in that, in order to expand the raw material base and increase the yield of the target product, δϋβηβ Ыpp semen tacks. 25