CN104151385B - A kind of method extracting cyclic adenosine monophosphate and application - Google Patents
A kind of method extracting cyclic adenosine monophosphate and application Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 54
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 title claims abstract description 20
- 238000001556 precipitation Methods 0.000 claims abstract description 55
- 238000000855 fermentation Methods 0.000 claims abstract description 49
- 230000004151 fermentation Effects 0.000 claims abstract description 49
- 239000007788 liquid Substances 0.000 claims abstract description 40
- 239000006228 supernatant Substances 0.000 claims abstract description 22
- 239000003960 organic solvent Substances 0.000 claims abstract description 20
- 230000033228 biological regulation Effects 0.000 claims abstract description 11
- 239000000047 product Substances 0.000 claims abstract description 11
- 238000005119 centrifugation Methods 0.000 claims abstract description 10
- 239000012141 concentrate Substances 0.000 claims abstract description 6
- 239000003513 alkali Substances 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 47
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 28
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 23
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 21
- 238000005516 engineering process Methods 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 16
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 16
- 239000000706 filtrate Substances 0.000 claims description 16
- 230000008569 process Effects 0.000 claims description 11
- 238000001291 vacuum drying Methods 0.000 claims description 11
- 229910052799 carbon Inorganic materials 0.000 claims description 9
- 238000002347 injection Methods 0.000 claims description 9
- 239000007924 injection Substances 0.000 claims description 9
- 239000008213 purified water Substances 0.000 claims description 8
- 238000001223 reverse osmosis Methods 0.000 claims description 7
- 239000002904 solvent Substances 0.000 claims description 7
- 230000008719 thickening Effects 0.000 claims description 7
- 238000004090 dissolution Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
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- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
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- 239000011591 potassium Substances 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
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- 239000008103 glucose Substances 0.000 description 3
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
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- 108010080698 Peptones Proteins 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
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- 238000012546 transfer Methods 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000186063 Arthrobacter Species 0.000 description 1
- 241001524201 Arthrobacter crystallopoietes Species 0.000 description 1
- 241000167367 Arthrobacter sp. A302 Species 0.000 description 1
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 1
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
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- 241000185994 Pseudarthrobacter oxydans Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 1
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- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 1
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Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
The invention discloses a kind of method extracting cyclic adenosine monophosphate and application, belong to bioactive substance extractive technique field.Method provided by the present invention is centrifugation acquisition supernatant after the pH utilizing alkali liquor regulation fermentation liquid, carry out after concentrated supernatant decolouring for the first time, utilize organic solvent fractional precipitation destaining solution, carry out second time after re-dissolved gained precipitation to decolour, the second destaining solution is concentrated after microporous filter, adding the second destaining solution after organic solvent deposit concentrates, centrifugation also obtains finished product after being vacuum dried precipitation.Method provided by the present invention may replace the exchange of existing ion and resin chromatography technique, it is possible to decrease wastewater flow rate 82.5%, and the production cycle is greatly shortened simultaneously, shortens the production time of 36%；Prepared cyclic adenosine monophosphate stay in grade, purity, up to more than 97%, can be widely applied to the fields such as medicine, food, feedstuff.
Description
Technical field
The present invention relates to a kind of method extracting cyclic adenosine monophosphate and application, belong to microbial fermentation product extractive technique field.
Background technology
Adenosine cyclophosphate is the second message,second messenger's material participating in regulation cell function, and its effect widely, can make myocardial contraction strengthen,
Causing elevation of the blood pressure, cardiac output increases.And can diastole smooth muscle, coronary artery dilator blood vessel, improve liver function, promote god
Divide and convert paracytic function through regeneration, suppression skin outer layer epithelial cell, promote the oxidasic activity of respiratory chain and change
Kind myocardial ischemia etc..
At present the main production process of cyclic adenosine monophosphate is chemical synthesis, also exists that yield is low, cost is high, and toxic and side effects is big
Shortcoming；In order to overcome these shortcomings, cyclic adenosine monophosphate is prepared in a kind of biological method of exploitation becomes research tendency, currently mainly has red
Fructus Jujubae extraction method and two kinds of research directions of biological fermentation process.And both approaches is compared, biological fermentation process has and is not limited by raw material,
Raw material is cheap and easily purchases, and scale is easily amplified, and constant product quality, and cost have great advantage, therefore this method
It it is a kind of method that replacement chemical synthesis is most potential.
At present the technique in terms of fermentative Production cyclic adenosine monophosphate product extraction is mainly ion exchange and adsorption chromatography, this side
The shortcoming of method maximum is that wastewater flow rate is big, and this is also that all resins separate the common problem existed, along with the raising of environmental requirement, useless
Cost of water treatment also begins to rise, and therefore in terms of economic and social benefit angle, develops a kind of method substituting resin separation and just shows
Obtain reasonably necessary.
Summary of the invention
For solving the problems referred to above, the invention provides a kind of method extracting cyclic adenosine monophosphate from fermentation liquid, the technology taked
Scheme is as follows:
It is an object of the invention to provide a kind of method extracting cyclic adenosine monophosphate from fermentation liquid, the method is regulation fermentation liquid
After pH, centrifugation obtains supernatant, carries out decolouring for the first time, utilize organic solvent fractional precipitation destaining solution after concentrated supernatant,
Carry out second time after re-dissolved gained precipitation to decolour, after microporous filter, concentrate the second destaining solution, after adding organic solvent deposit concentration
The second destaining solution, centrifugation also obtains finished product after being vacuum dried precipitation.
The step of described method is as follows:
1) utilize the pH to 8.0-8.5 of alkali liquor regulation fermentation liquid, after being centrifuged, obtain supernatant；
2) concentration step 1) gained supernatant, and carry out desolventing technology, it is thus achieved that the first destaining solution；
3) organic solvent is utilized to step 2) the first destaining solution of gained carries out fractional precipitation, and partly precipitated is collected in centrifugation；
Described partly precipitated is the precipitation of organic solvent concentration 40%-50%.
4) purified water dissolving step 3 is utilized) partly precipitated of gained, carry out second time desolventing technology after dissolving, after microporous filter
Obtain filtrate；
5) concentration step 4) gained filtrate, add organic solvent and carry out precipitation process, centrifugal separately winning takes precipitation；
6) vacuum drying step 5) gained precipitation acquisition finished product.
Step 1) described fermentation liquid is arthrobacterium fermentation liquid；Described alkali liquor is sodium or the hydroxide of potassium or carbonate compound solution；
Described centrifugal, it is centrifugal 5-6min under 4500-5000rpm.
Step 2) described concentration, it is concentrating under reduced pressure, vacuum is-0.065～-0.075MPa, and thickening temperature is 45-50 DEG C, concentrates
Final concentration 20-25g/L；Described desolventing technology, uses craboraffin, processes 15-20min, activated carbon under the conditions of 55-60 DEG C
Addition is the 1% of concentrated solution volume.
Step 3) described fractional precipitation, it is to use dehydrated alcohol or acetone, carries out by the way of stream adds；Described part of collecting is sunk
Form sediment, be the precipitation collecting organic solvent concentration 40%-50%.
Step 4) described purified water, it is two-pass reverse osmosis water, electrical conductivity≤2 μ S/cm, amount of water is 5 times of precipitation quality；Described
Desolventing technology for the second time, is decolour under normal temperature condition 15min with injection active carbon, and the addition of injection active carbon is for precipitating quality
6%.
Step 5) described concentration is in vacuum-0.065～-0.075MPa, under conditions of temperature 45-50 DEG C, by the volume of filtrate
It is concentrated into the 1/3 of original volume；Described precipitation process, is that to add dehydrated alcohol or acetone to solvent volume concentration be 70%.
Step 6) described vacuum drying, baking temperature is 45 DEG C, drying time 10h.
Specifically comprising the following steps that of described method
1) utilize the pH to 8.0-8.5 of sodium hydroxide solution regulation arthrobacterium fermentation liquid, then fermentation liquid is centrifuged under 5000rpm
5min, it is thus achieved that supernatant；
2) by step 1 under conditions of vacuum-0.065～-0.075MPa, thickening temperature 45-50 DEG C) gained supernatant concentration is extremely
Concentration is 25g/L, adds the craboraffin of concentrated solution volume 1%, at 60 DEG C, desolventing technology 15min, go in concentrated solution
After activated carbon, it is thus achieved that the first destaining solution；
3) to step 2 in the way of stream adds) the first destaining solution of gained adds dehydrated alcohol or acetone carries out fractional precipitation process,
Collect the precipitation of organic solvent concentration 40%-50%；
4) to step 3) partly precipitated of gained adds the two-pass reverse osmosis water dissolution precipitation of the electrical conductivity≤2 μ S/cm of 5 times of quality,
Add precipitation quality 6% injection active carbon, at room temperature desolventing technology 15min and have microporous filter obtain filtrate；
5) in vacuum-0.065～-0.075MPa, under conditions of temperature 45-50 DEG C, by step 4) volume concentration of gained filtrate
To the 1/3 of original volume, adding dehydrated alcohol or acetone to solvent volume concentration is 70%, and under 8000rpm, centrifugal 20min, obtains
Must precipitate；
6) under the conditions of 45 DEG C, vacuum drying step 5) gained precipitation 10h.
Described method is for extracting cyclic adenosine monophosphate from microbial fermentation solution.
The present invention is preferably used tube centrifuge to the separation of fermentation liquid；The preferred single-action of concentrating under reduced pressure equipment or multiple-effect decompression evaporator；
Organic solvent deposit separates preferred explosion-proof type cloth bag loading and unloading material centrifuge, decarburization preferred tubular type decarburizing machine.
The method have the benefit that
1, the method taking twice crystalline deposit, substitution ion exchange and resin chromatography technique, reduce wastewater flow rate more than 70%.
2, tube centrifuge is used intermediate products to be separated, compared to traditional pressure filter and membrane filter system, separating effect
Good, continuous production is strong, reduces labor intensity, using water wisely.
3, the production cycle is greatly shortened, and by original 72 hours, foreshortens to 48 hours.
4, product with stable quality, purity is not less than 95%, and content of beary metal meets enterprise-quality standard.Yield is more than 72%.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be further described, but the present invention should not be limited by the examples.
Method therefor of the present invention, reagent and instrument, without special instruction, be in the art conventional method, reagent and
Instrument.
The preparation of embodiment 1 fermentation liquid
Fermentation liquid used by the present invention is arthrobacterium fermentation liquid, wherein, and preferred strain Arthrobacter crystallopoietes,
Arthrobacter oxidans, Arthrobacter CCTCC NO:M2013431, the fermentation liquid of Arthrobacter sp.A302.
Present embodiments provide the preparation method of a kind of above-mentioned bacterial strains fermentation liquid.
The present embodiment used medium particular make-up is as follows:
Slant medium (g/L): glucose 10, peptone 10, yeast extract 10, Carnis Bovis seu Bubali cream 10, NaCl 3, agar 20.
pH 6.8。
Seed culture medium (g/L): glucose 10, peptone 10, yeast extract 10, Carnis Bovis seu Bubali cream 10, NaCl 3.pH 6.8.
Fermentation medium (g/L): glucose 30, K2HPO415, KH2PO45, MgSO40.1, carbamide 10, biotin 0.005,
CoCl20.005, NaCl 0.4, hypoxanthine 5, pH 7.0.
Fermentation condition is as follows:
-80 DEG C of Refrigerator store strains are inoculated in slant medium activation, cultivate 48h for 30 DEG C.Lawn on slant medium connects
Plant in seed culture medium, 30 DEG C, 280r/min shaking table cultivation 24h；Cultured seed culture medium is connect with the inoculum concentration of 8%
Enter fermentation medium, 30 DEG C, 280r/min shaking table cultivation 72h.Above seed culture medium and fermentation medium all use 500mL
Shaking flask, liquid amount is 30mL.
Embodiment 2
Present embodiments providing a kind of method using ion exchange and resin chromatography method to extract adenosine phosphate, step is as follows:
1) the fermentation liquid 1L prepared by Example 1, fermentation unit 3g/L, under 5000rpm rotating speed, centrifugal 5min, collects
Supernatant, adjusts pH to 2.0 with concentrated hydrochloric acid.
2) resin used by ionic energy transfer is 001 × 7 type cation exchange resin, and resin dress column volume 200mL, with 4%
HCl treatment becomes Hydrogen.Upper column flow rate 0.3 column volume per hour per hour, upper prop terminate after again by the purified water of 2 times of bed volumes
Binder, binder ibid, collects eluent 450mL with 0.1N sodium hydroxide eluting, elution flow rate after terminating.
3) resin chromatography chromatographic resin is macroporous adsorbent resin HP-20, resin dress column volume 300mL, will collect and wash after handing over
De-liquid adds in resin, and loading flow velocity 0.3 column volume per hour, uses 3 times of column volume purified water and 5 times of posts after end of the sample
20% methanol solution stepwise elution of volume, collects methanol solution elution fraction, and elution flow rate is ibid.
4) eluent is concentrated in decompression evaporator by concentration, vacuum-0.065～-0.075MPa, evaporating temperature 40-45 DEG C,
It is concentrated into the 1/5 of fermentating liquid volume.
5) crystallization adds dehydrated alcohol to dense degree 70%, stirred crystallization 12 hours eventually toward concentrated solution
6) separation drying crystalline utilizes the wet crystal of centrifuge isolated after completing, and is subsequently placed in vacuum drying oven and is dried, dry
Dry temperature 40-45 DEG C.
Embodiment 3
1) the fermentation liquid 1L prepared by Example 1, fermentation unit 4g/L, under 5000rpm rotating speed, centrifugal 5min, collects
Supernatant, adjusts pH to 3.0 with concentrated hydrochloric acid.
2) resin used by ionic energy transfer is 001 × 7 type cation exchange resin, and resin dress column volume 200mL, with 4%
HCl treatment becomes Hydrogen.Upper column flow rate 0.3 column volume per hour per hour, upper prop terminate after again by the purified water of 2 times of bed volumes
Binder, binder ibid, collects eluent 450mL with 0.1N sodium hydroxide eluting, elution flow rate after terminating.
3) resin chromatography chromatographic resin is macroporous adsorbent resin HP-20, resin dress column volume 300mL, will collect eluting after handing over
Liquid adds in resin, and loading flow velocity 0.3 column volume per hour, uses 3 times of column volume purified water and 5 times of cylinders after end of the sample
20% long-pending methanol solution stepwise elution, collects methanol solution elution fraction, and elution flow rate is ibid.
4) eluent is concentrated in decompression evaporator by concentration, vacuum-0.065～-0.075MPa, evaporating temperature 40-45 DEG C,
It is concentrated into the 1/5 of fermentating liquid volume.
5) crystallization adds dehydrated alcohol to dense degree 70%, stirred crystallization 12 hours eventually toward concentrated solution
6) separation drying crystalline utilizes the wet crystal of centrifuge isolated after completing, and is subsequently placed in vacuum drying oven and is dried, dry
Dry temperature 40-45 DEG C.
Embodiment 4
Present embodiments providing a kind of method extracting cyclic adenosine monophosphate from fermentation liquid, step is as follows:
1) the fermentation liquid 1L prepared by Example 1, fermentation unit 4g/L utilize the pH of sodium hydroxide solution regulation fermentation liquid extremely
8.0-8.5, then by fermentation liquid centrifugal 5min under 5000rpm, it is thus achieved that supernatant；
2) by step 1 under conditions of vacuum-0.065～-0.075MPa, thickening temperature 45-50 DEG C) gained supernatant concentration is extremely
Concentration is 25g/L, adds the craboraffin of concentrated solution volume 1%, at 60 DEG C, desolventing technology 15min, go in concentrated solution
After activated carbon, it is thus achieved that the first destaining solution；
3) to step 2 in the way of stream adds) the first destaining solution of gained adds dehydrated alcohol or acetone carries out fractional precipitation process,
Collect the precipitation of organic solvent concentration 40%-50%；
4) to step 3) partly precipitated of gained adds the two-pass reverse osmosis water dissolution precipitation of the electrical conductivity≤2 μ S/cm of 5 times of quality,
Add precipitation quality 6% injection active carbon, at room temperature desolventing technology 15min and have microporous filter obtain filtrate；
5) in vacuum-0.065～-0.075MPa, under conditions of temperature 45-50 DEG C, by step 4) volume concentration of gained filtrate
To the 1/3 of original volume, adding dehydrated alcohol or acetone to solvent volume concentration is 70%, and under 8000rpm, centrifugal 10min, obtains
Must precipitate；
6) under the conditions of 45 DEG C, vacuum drying step 5) gained precipitation 10h.
Embodiment 5
Present embodiments providing a kind of method extracting cyclic adenosine monophosphate from fermentation liquid, step is as follows:
1) the fermentation liquid 1L prepared by Example 1, fermentation unit 4g/L utilize the pH of sodium hydroxide solution regulation fermentation liquid extremely
8.0-8.5, then by fermentation liquid centrifugal 5min under 5000rpm, it is thus achieved that supernatant；
2) by step 1 under conditions of vacuum-0.065～-0.075MPa, thickening temperature 45-50 DEG C) gained supernatant concentration is extremely
Concentration is 25g/L, adds the craboraffin of concentrated solution volume 1%, at 60 DEG C, desolventing technology 10min, go in concentrated solution
After activated carbon, it is thus achieved that the first destaining solution；
3) to step 2 in the way of stream adds) the first destaining solution of gained adds dehydrated alcohol or acetone carries out fractional precipitation process,
Collect the precipitation of organic solvent concentration 40%-50%；
4) to step 3) partly precipitated of gained adds the two-pass reverse osmosis water dissolution precipitation of the electrical conductivity≤2 μ S/cm of 5 times of quality,
Add precipitation quality 6% injection active carbon, at room temperature desolventing technology 15min and have microporous filter obtain filtrate；
5) in vacuum-0.065～-0.075MPa, under conditions of temperature 45-50 DEG C, by step 4) volume concentration of gained filtrate
To the 1/3 of original volume, adding dehydrated alcohol or acetone to solvent volume concentration is 70%, the centrifugal 15min with under 8000rpm,
Obtain precipitation；
6) under the conditions of 45 DEG C, vacuum drying step 5) gained precipitation 10h.
Embodiment 6
Present embodiments providing a kind of method extracting cyclic adenosine monophosphate from fermentation liquid, step is as follows:
1) the fermentation liquid 1L prepared by Example 1, fermentation unit 4g/L utilize the pH of sodium hydroxide solution regulation fermentation liquid extremely
8.0-8.5, then by fermentation liquid centrifugal 5min under 4000rpm, it is thus achieved that supernatant；
2) by step 1 under conditions of vacuum-0.065～-0.075MPa, thickening temperature 45-50 DEG C) gained supernatant concentration is extremely
Concentration is 25g/L, adds the craboraffin of concentrated solution volume 1%, at 50 DEG C, desolventing technology 15min, go in concentrated solution
After activated carbon, it is thus achieved that the first destaining solution；
3) to step 2 in the way of stream adds) the first destaining solution of gained adds dehydrated alcohol or acetone carries out fractional precipitation process,
Collect the precipitation of organic solvent concentration 40%-50%；
4) to step 3) partly precipitated of gained adds the two-pass reverse osmosis water dissolution precipitation of the electrical conductivity≤2 μ S/cm of 5 times of quality,
Add precipitation quality 6% injection active carbon, at room temperature desolventing technology 15min and have microporous filter obtain filtrate；
5) in vacuum-0.065～-0.075MPa, under conditions of temperature 45-50 DEG C, by step 4) volume concentration of gained filtrate
To the 1/3 of original volume, adding dehydrated alcohol or acetone to solvent volume concentration is 70%, the centrifugal 15min with under 8000rpm,
Obtain precipitation；
6) under the conditions of 45 DEG C, vacuum drying step 5) gained precipitation 10h.
Embodiment 7
The present embodiment utilizes the purity of high effective liquid chromatography for measuring embodiment 2-6 gained sample, and result is as shown in table 1；
Table 1 embodiment 2-6 prepares purity and the extraction ratio of cyclic adenosine monophosphate
| Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | |
| Purity | 99 | 97 | 97 | 99 | 98 |
| Extraction ratio | 65% | 62 | 72% | 72.5 | 71% |
As it can be seen from table 1 the purity of the method products therefrom of embodiment 4-6 with used by embodiment 2 and 3 ion exchange and
Resin chromatography method does not has marked difference, but the extraction ratio of product but exchanges apparently higher than the ion used by embodiment 2 and 3 and tree
Fat chromatography.Illustrate that method provided by the present invention is more efficient than ion exchange and resin chromatography method.
Table 2 is that embodiment 2-6 prepares the wastewater flow rate used by cyclic adenosine monophosphate and time.
Table 2 embodiment 2-6 prepares the waste water consumption of cyclic adenosine monophosphate and time used
| Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | |
| Wastewater flow rate | 10L | 12L | 2.5L | 2.1L | 2.3L |
| Preparation total time | 73 | 72 | 49 | 47 | 49 |
From Table 2, it can be seen that the wastewater flow rate used by embodiment 4-6 is considerably less than the wastewater flow rate used by embodiment 2-3, meanwhile,
The preparation time used the most substantially shortens.Compared with ion exchange and resin chromatography method, method therefor of the present invention can be saved
75%-82.5% uses water, shortens the time of 31%-36%.
Although the present invention is open the most as above with preferred embodiment, but it is not limited to the present invention, any is familiar with this technology
People, without departing from the spirit and scope of the present invention, can do various change and modification, and therefore protection scope of the present invention should
Should be with being as the criterion that claims are defined.
Claims (10)
1. the method extracting cyclic adenosine monophosphate, it is characterized in that, after the pH of regulation fermentation liquid, centrifugation obtains supernatant, carry out after concentrated supernatant decolouring for the first time, utilize organic solvent fractional precipitation destaining solution, carry out second time after re-dissolved gained precipitation and decolour, after microporous filter, concentrate the second destaining solution, adding the second destaining solution after organic solvent deposit concentrates, centrifugation also obtains finished product after being vacuum dried precipitation.
2. method described in claim 1, it is characterised in that step is as follows:
1) utilize the pH to 8.0-8.5 of alkali liquor regulation fermentation liquid, after centrifugation, collect supernatant；
2) concentration step 1) gained supernatant, and carry out desolventing technology, it is thus achieved that the first destaining solution；
3) organic solvent is utilized to step 2) the first destaining solution of gained carries out fractional precipitation, and partly precipitated is collected in centrifugation；Described partly precipitated is the precipitation of organic solvent concentration 40%-50%；
4) purified water dissolving step 3 is utilized) partly precipitated of gained, carry out second time desolventing technology after dissolving, after microporous filter, obtain filtrate；
5) concentration step 4) gained filtrate, add organic solvent and carry out precipitation process, centrifugation obtains precipitation；
6) vacuum drying step 5) gained precipitation acquisition finished product.
3. method described in claim 2, it is characterised in that step 1) described fermentation liquid is arthrobacterium fermentation liquid；Described alkali liquor is sodium or the hydroxide of potassium or carbonate compound solution；Described centrifugal, it is centrifugal 5-6min under 4500-5000rpm.
4. method described in claim 2, it is characterised in that step 2) described concentration, it is concentrating under reduced pressure, vacuum is-0.065～-0.075MPa, and thickening temperature is 45-50 DEG C, concentrates final concentration 20-25g/L；Described desolventing technology, uses craboraffin, processes 15-20min under the conditions of 55-60 DEG C, and activated carbon addition is the 1% of concentrated solution volume.
5. method described in claim 2, it is characterised in that step 3) described fractional precipitation, it is to use dehydrated alcohol or acetone, carries out by the way of stream adds；Described collection partly precipitated, is the precipitation collecting organic solvent concentration 40%-50%.
6. method described in claim 2, it is characterised in that step 4) described purified water, it is two-pass reverse osmosis water, electrical conductivity≤2 μ S/cm, amount of water is 5 times of precipitation quality；Described second time desolventing technology, is decolour under normal temperature condition 15min with injection active carbon, and the addition of injection active carbon is precipitate quality 6%.
7. method described in claim 2, it is characterised in that step 5) described concentration is in vacuum-0.065～-0.075MPa, under conditions of temperature 45-50 DEG C, by the 1/3 of the volume concentration of filtrate to original volume；Described precipitation process, is that to add dehydrated alcohol or acetone to solvent volume concentration be 70%.
8. method described in claim 2, it is characterised in that step 6) described vacuum drying, baking temperature is 45 DEG C, drying time 10h.
9. method described in claim 2, it is characterised in that specifically comprise the following steps that
1) pH to 8.0-8.5 of sodium hydroxide solution regulation arthrobacterium fermentation liquid is utilized, then by fermentation liquid centrifugal 5min under 5000rpm, it is thus achieved that supernatant；
2) in vacuum-0.065～-0.075MPa, by step 1 under conditions of thickening temperature 45-50 DEG C) gained supernatant concentration to concentration is 20-25g/L, the craboraffin of concentrated solution volume 1% is added in concentrated solution, at 60 DEG C, desolventing technology 15min, after removing activated carbon, it is thus achieved that the first destaining solution；
3) to step 2 in the way of stream adds) the first destaining solution of gained adds dehydrated alcohol or acetone carries out fractional precipitation process, collect the precipitation of organic solvent concentration 40%-50%；
4) to step 3) partly precipitated of gained adds the two-pass reverse osmosis water dissolution precipitation of the electrical conductivity≤2 μ S/cm of 5 times of quality, add the injection active carbon of precipitation quality 6%, at room temperature desolventing technology 15min obtain filtrate with microporous filter；
5) in vacuum-0.065～-0.075MPa, under conditions of temperature 45-50 DEG C, by step 4) volume concentration of gained filtrate is to the 1/3 of original volume, and adding dehydrated alcohol or acetone to solvent volume concentration is 70%, centrifugal 20min under 8000rpm, it is thus achieved that precipitation；
6) under the conditions of 45 DEG C, vacuum drying step 5) gained precipitation 10h.
10. method described in claim 1, for extracting cyclic adenosine monophosphate from microbial fermentation solution.
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| CN105541946A (en) * | 2016-03-11 | 2016-05-04 | 阎虎林 | Adenosine cyclophosphate crystalline compound |
| CN107417750B (en) * | 2017-05-16 | 2020-04-21 | 河南科技学院 | Method for extracting cyclic adenosine monophosphate from microbial fermentation liquid |
| CN109851651A (en) * | 2019-02-18 | 2019-06-07 | 江苏集萃工业生物技术研究所有限公司 | A kind of method that macroporous absorbent resin separates cyclic adenosine monophosphate from catalytic liquid |
| CN112300968B (en) * | 2020-11-19 | 2023-10-27 | 河南巨龙生物工程股份有限公司 | Arthrobacter for producing adenosine cyclophosphate and application thereof |
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| CN102268469A (en) * | 2010-06-04 | 2011-12-07 | 南京工业大学 | Preparation method of cyclic adenosine monophosphate |
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