SU1114697A1 - Method for preparing biomass of fodder yeast - Google Patents

Abstract

A method for producing biomass of fodder yeast, which involves hydrolysis of pre-moistened straw at 160-180 ° C for 0.752, 5 h, cooling the substrate, introducing into it sources of nitrogen and phosphorus with subsequent cultivation of the biomass producer at 35-40 ° C, and applying biomass at 35-40 ° C, and applying biomass at 35-40 ° C, and applying biomass at 35–40 ° C, and adding biomass to the biomass at 35–40 ° C and 35 80-90 ° C for 1-2 hours, in order to increase the biomass yield, after hydrolyzing the straw, the hydrolyzate is sampled, blown with live steam for 0.5-1.0 hours, and as a biomass producer Use strain Trichosporon pullulans WGI

Description

"The invention relates to the microbiological industry and can be used in fodder production with the enrichment of feed protein, a known method of producing protein feed, involving the hydrolysis of straw acid previously soaked in rastication by steaming for 3 hours with 20-24 hours at 27 -30 ° C l. The disadvantage of this method is the low biomass yield. The closest to the technical essence and the achieved effect to the proposed is a method of obtaining biomass of fodder yeast, involving hydrolysis of pre-moistened straw at 160180®С for 0.75-2.5 h, cooling the substrate, adding nitrogen and phosphorus sources to it, followed by growing the biomass producer at 35-40 ° C and keeping it at 8090 ° C for 1-2 hours. Baking yeast is used for pulling {j The disadvantage of this method is the low biomass yield. The purpose of the invention is to increase the output and biomass. The goal is achieved by the fact that according to the method of producing biomass of fodder yeast, a hydrolysis of pre-moistened straw at 160-180 ° C for 0.75-2.5 h is provided for the hydrolysis of the substrate, the introduction of nitrogen and phosphorus sources into it, followed by cultivation of the producer biomass at 35–40 ° C and holding at 80–90 ° C for 1–2 h, after hydrolysis of the straw, the hydrolyzate is sampled, rinsed with live steam for 0.5–1.0 h, and as a biomass producer strain Triehosporon pullulans VGI (78) -6. An increase in biomass yield is achieved by using free sugar containing sugar, which has not been previously utilized. The free gdrolizat is flushed with live steam to distill the furfural. Necessary additives to the hydrolyzate are salts of nitrogen and phosphorus. The process of growing yeast is carried out in the usual way in fermenters using the Trichosporon pullulans strain 972 WGI {78) -6 specifically found for rice straw hydrolyzate. To neutralize the resulting yeast suspension is heated. Rice straw is soaked in water for 20 hours to make it 75-80% moisture. Then, it is loaded into the heated hydrolysis apparatus, and when it reaches the specified temperature, a certain time is required for weak hydrolysis. The resulting free hydrolyzate is taken from the bottom of the hydrolyzate. Characteristics of the obtained hydrolyzate are given in table. 1. The free hydrolyzate is flushed with live steam to reduce the furfural content, then nutrient salts of nitrogen 5% and phosphorus 2.5% are calculated based on the sugar contained in the free hydropyzate, cooled and inoculated with Trichosporon pullulans strain VGI (78) -6. The strain Trichosporon pullulans VGI (78) -6 is selected as a spontaneous mutant on sulfur-deficient media and can be used as a producer of forage biomass when it is extruded on the hydrolyzates of agricultural waste (cotton husk, rice husk) and rice straw hydrolyzate. Strain Trichosporon pullulans VGI (78) -6 non-pathogenic deplanation number in the Central Museum of Industrial Microorganisms of the Institute VNIIGenetics at 407. Cultural and morphological characteristics of the strain. On the malt sulsegar forms small-folded Crea colonies, on the malt wort a 1-day culture forms a precipitate of film islets. Cerebone cells single and in small eyelids. Forms mycelium and pseudo-mycelium. Biochemical properties. Supports glucose and galactose. Culture stimulates glucose, galactose, acharose, maltose, raffinose, starch, xylose, arabinose, ethanol, glycerin, dulcite, mannitol, sorbitol, and potassium nitrate. Does not absorb lactose. Activity (productivity) of the shtam, as well as other production agents of the strain. Tamm productivity when cultivated on rice straw straw tern with a concentration of 0.72% RV is 1.3 g of absolutely dry yeast per liter of medium per hour. The economic coefficient is 0.65 with a crude protein content of 50.2%. The specific growth rate of the strain is 0.16. The productivity of the strain is determined under continuous cultivation conditions at the VNII hydrolysis unit in a 3-liter apparatus at 36-38 ° C, pH 4.4-4.6, the stirrer turnover rate is 1200 rpm, air consumption 4 l per 1 l of culture fluid . The strain is maintained by periodic transfers to wort agar and hydrolyzed agar in test tubes. Storage is carried out at room temperature. The composition of storage media is: wort agar with 8B strength, pH 5.6, agar-agar concentration 20 g / l. Hydrolyzate agar: wood hydrolyzate with a PB concentration of 1.5% contains 750 mg / l of mineral nitrogen and 350 MP / l, the pH value is adjusted to 6.0 with 5% ammonia. The agar-agar concentration of 30 g / l, reseeding on the hydrolyzate-agar is carried out 1 time in 7-10 days. It is possible to store it under the vaseline oil for 0.5 g. Example 1.1.4 kg of rice straw is moistened in a container with water. The straw is autoclaved at 160 ° C for 2.5 hours. As a result of autoclaving, 4.1 l of free hydrolyzate with sugar content is collected. ra 0.86% and furfural 0.04%. For 0.5 h, the free hydrolyzate is blown with sharp steam and nutrient salts are added. In cooled to 38 C free hydrolyzate seeded HGI (78) -6 yeast. Extraction is carried out at 36 ° C for 10 hours. The resulting yeast suspension with a concentration of 26.8 g / l is held for 2 hours. Example 2. 1.4 kg of rice straw are moistened and autoclaved at 170s for 1.5 h. As a result of autoclaving, 3 l of free hydrolyzate with a sugar content of 0.53% and furfural of 0.06% is taken. Steam scavenging is carried out for 0.75 hours, nutrient salts are added. BGI (78) -6 yeast was boiled at 37 ° C for 6 hours. The resulting suspension with a concentration of 16.5 g / l was held at 85 ° C for 1.5 hours. Example 3. 1.4 kg of rice straw is moistened for 0.75 hours. The resulting 3 liters of free hydrolyzate contain 0.88% sugar and 0.08% furfural. Steam flushing is carried out for 1 hour. Nutrient salts are applied. The removal of HGI (78) -6 yeast was carried out at 38 ° C for 8 hours. The resulting suspension with a concentration of 27.4 g / l was held at 90 ° C for 1 hour. In tab. 2 shows the data for comparing the proposed method with the known. Thus, the proposed method, in comparison with the known, allows increasing the yield of yeast biomass from 22 to 65%. Table 1

The content of sucrose by ebuliostatic method

The composition of monosaccharides by gas chromatography:

xylose

arabinose rhamnose

glucose

0.86-1.0

0.46 0.18 0.29

galactose

Free organic acids in terms of acetic acid

Furfural

Indicators

Concentration of sugar in the substrate,% Concentration of organic acids,%

Pressed Yeast Concentration

in appar.ate, g / l

Output and.with. biomass from carbon-containing substances (sugars and organic acids)

Protein content,%

Continued table. I

0.06

0.35 0.04-0.08

table 2

Strain WGI (78) -6

Baker's yeast

0.8-1.0 0.3-0.34

8-10

20-22,4 defined

Claims (2)

METHOD FOR PRODUCING FODDER YEAST BIOMASS, providing for the hydrolysis of pre-moistened straw at 160-180 ° C for '0.75- 2.5 hours, cooling the substrate, introducing sources of nitrogen and phosphorus into it, followed by growing the biomass producer at 35-4 ° C and holding it at 80-90 ° C for 1-2 hours, characterized in that, in order to increase the biomass yield , after hydrolysis of straw, the hydrolyzate is selected, it is blown with hot steam for 0.5-1.0 hours, and the VGI Trichosporon pullulans strain (78) -6 is used as a producer of biomass. B