GB800634A - Improvements in or relating to the preparation of 2-keto-l-gulonic acid - Google Patents
Improvements in or relating to the preparation of 2-keto-l-gulonic acidInfo
- Publication number
- GB800634A GB800634A GB21692/55A GB2169255A GB800634A GB 800634 A GB800634 A GB 800634A GB 21692/55 A GB21692/55 A GB 21692/55A GB 2169255 A GB2169255 A GB 2169255A GB 800634 A GB800634 A GB 800634A
- Authority
- GB
- United Kingdom
- Prior art keywords
- acid
- mixture
- aerobic conditions
- under aerobic
- idonate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/58—Aldonic, ketoaldonic or saccharic acids
- C12P7/60—2-Ketogulonic acid
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
2-Keto-l-gulonic acid is obtained by subjecting l-idonic acid or a non-toxic l-iodonate under aerobic conditions to the oxidizing system produced by cultivating a mixture of the two organisms Pseudomonas ATCC 11867 and Pseudomonas ATCC11868 under aerobic conditions on an otherwise sterile nutrient medium. It is stated that a living culture of each of the organisms has been deposited with the American Type Culture Collection in Washington, D.C., where they have been given the above numbers. The process may be effected in various ways, thus the mixture may be grown under aerobic conditions on an otherwise sterile nutrient medium containing l-idonic acid or the l-idonate. Alternatively, to such a medium in which growth has been established l-idonic acid or the l-idonate may be added. The reaction may also be carried out by subjecting l-idonic acid or a non-toxic l-idonate to a suspension of cells so grown or to a cell-free broth in which the mixture has been grown. The term non-toxic l-idonate includes salts of metals, e.g. alkali metals, ammonia and amines, and esters of simple alcohols, e.g. of methanol or ethanol, with l-idonic acid. The mixture of organisms may readily be grown on standard nutrient media, thus a liquid nutrient medium containing a source of carbohydrate such as dextrose, inorganic salts such as potassium phosphate and magnesium sulphate, a protein source such as peptone, and growth-promoting substances such as yeast extract, may be used. The cultivation may also be readily carried out on ordinary nutrient agar slants. In one method a solution containing nutrients such as dextrose and yeast extract is charged with l-idonic acid or a nontoxic l-idonate and inoculated with a mixed culture of the organisms which has previously been grown under aerobic conditions on either agar slants or a liquid nutrient medium. The fermentation broth is shaken under aerobic conditions at between about 25 DEG C. and 30 DEG C. and generally good conversion is produced in about 34 hours by 1 cc. of a 48-hour inoculum in 100 cc. of fermentation mixture and the progress of the reaction may be followed by means of a colour test using orthophenylenediamine dihydrochloride in the form of a 2 1/2 per cent aqueous solution. In an example a culture of a mixture of ATCC 11867 and ATCC 11868 is grown for 48 hours under aerobic conditions on a nutrient broth containing 0.25 per cent yeast extract. One cc. of this broth is inoculated into 100 cc. of an aqueous fermentation mixture of pH 7.0 and having the following composition by weight: 2 per cent sodium l-idonate-, 0.1 per cent dextrose and 0.5 per cent yeast extract. The mixture is maintained at 28 DEG C. under aerobic conditions and after 34 hours 2-keto-gulonic acid is isolated by the following procedure: the broth is concentrated to about one quarter of its original volume by evaporation, acetic acid is added to reduce the pH to about 4 and sodium hydroxide is then added to raise the pH to about 7.5. Methyl alcohol is then added and the sodium 2-keto-l-gulonate which is precipitated is filtered off and treated with a non-oxidizing strong acid such as hydrochloric acid to yield the free acid. The organisms used are stated to be Gram-negative, straightcelled bacilli and other characteristics of the organisms are described.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US800634XA | 1954-08-10 | 1954-08-10 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB800634A true GB800634A (en) | 1958-08-27 |
Family
ID=22155022
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB21692/55A Expired GB800634A (en) | 1954-08-10 | 1955-07-27 | Improvements in or relating to the preparation of 2-keto-l-gulonic acid |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB800634A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003033448A1 (en) * | 2001-10-10 | 2003-04-24 | Basf Aktiengesellschaft | Method for isolating salts of organic acids from a fermentation broth and for releasing the organic acid |
-
1955
- 1955-07-27 GB GB21692/55A patent/GB800634A/en not_active Expired
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003033448A1 (en) * | 2001-10-10 | 2003-04-24 | Basf Aktiengesellschaft | Method for isolating salts of organic acids from a fermentation broth and for releasing the organic acid |
DE10149869A1 (en) * | 2001-10-10 | 2003-04-24 | Basf Ag | Isolating salts of organic acids from fermentation broth, e.g. 2-keto-L-gulonate for production of Vitamin C, involves partial evaporative crystallization followed by displacement precipitation |
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