SU1013852A1 - Urobilin identification method - Google Patents

Abstract

METHOD OF IDENTIFICATION D-UROBILIN in a biological fluid, characterized in that, in order to reduce the time-study, a fluid sample is placed in a quartz cell, irradiated with ultraviolet light in the range of 300-385 nm. and by fluorescence in the red or orange region identify .0-urobilin. g

Description

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The invention relates to medicine, in particular to the study of biological substances, and is intended to determine O-urobilin in biological fluids.

A known method for the identification of D-urobilin by electrophoresis of a sample of biological fluid 1.

However, this method is laborious, time-consuming (time-consuming up to 24 hours). It does not allow conducting mass surveys. It is a little specific in relation to the D-uroblin fraction.

The known method of defining O -ubilin is based on the use of the reaction with ferric chloride 2.

However, the implementation of this method requires a lot of time (up to 2.5 hours; as well as a certain amount of chemical reagents of the Second World War, which limits the possibility of mass examination of patients.

The aim of the invention is to reduce the research time.

The goal is achieved by the fact that according to the D-urobilin identification method in a biological fluid, a fluid sample is placed in a quartz cell, irradiated with ultraviolet light (UV) in the range of 300385, and D-urobilin is identified by fluorescence in the red or orange Region.

The method is carried out as follows.

Take 2.5 ml of biological fluid (serum, urine), place it in a quartz cuvette, irradiate with ultraviolet light in the range of 300-385 nm for 1 min and evaluate it using a fluorometer of any brand. Visual assessment in a darkened camera can also be used.

When registering red and orange fluorescence, the presence of the D-aurobilin is determined. An orange-red glow indicates a high concentration of D-urobilin. In the absence of a D-aurobilin, green fluorescence is observed.

Example. Sick 57 years. The initial diagnosis is viral hepatitis. The disease is acute: jaundice, abdominal pain, nausea, vomiting, itching. Viral diagnosis

hepatitis is removed, pancreatic head cancer diagnosed.

To confirm the diagnosis, the patient was diagnosed with noD urobilin by the proposed method. A patient is taken 2.5 ml mo., Placed in a quartz cuvette in the path of ultraviolet light with a wavelength of 300385 nm for 1 min. Orange-red fluorescence is recorded visually, which indicates the presence of a large amount of D-urobilin in the patient and confirms the diagnosis. The correctness of the diagnosis is confirmed by mesobiliviolin reactions, which is used to determine the exact content of D-urobilin in the urine and which, in this case, is 40%.

PRI me R 2. A 45-year-old patient, was admitted to a therapeutic clinic with an uneasy diagnosis. A state of average weight, complains of lack of appetite, poor sleep. No other complaints.

Produced clinical and laboratory examination. The state of the cardiovascular system, the lungs of the stomach, and the kidneys are normal. A patient is prescribed a laboratory test, among which is the study of the presence of D-urobilin in biological fluids. The study was conducted in the laboratory of the Department of Infectious Diseases. A patient is taken from the cubital vein 7 ml of blood; 2.5 sy: blood cranks. Placed in a quartz cuvette in the path of ultraviolet light with a wavelength of 300-385 nm for 1 y. Red fluorescence is visually recorded, which indicates the presence of D-urobilin in the patient. Additionally, a mesobilivioline reaction is carried out, the results of which confirm the presence of urobilin) in an amount of 75%. Based on the data and additional clinical studies, the diagnosis is primary liver cancer.

The proposed method conducted 620 studies of biological fluids of serum and urine) on O urobilin.

The results of the indication of D-urobilin in biological fluids are presented in the table.

Patients with tumor jaundice.

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285 3. 10138 In 50 patients with tumor jaundices, an elevated concentration of this fraction was determined by red and orange fluorescence. In all patients, the reliability of the method was confirmed by mesobiliviolinum. by reaction The blood serum of donors, as well as the serum of patients with viral hepatitis. not containing O-urobilin, fluoresced in green. 10 The time for determination of D-urobilin by the proposed method is 20 minutes, 2 compared to 2 by the known method. There is no need for chemical reagents. To do this, it is sufficient to have a UV source and a fluorimeter of any mark | and in the absence of the latter, a visual assessment is possible in the closed chamber. Simplicity, cost effectiveness, speed allow the proposed method to be used for the rapid and reliable determination of the D-urobilin fraction both in the hospital and in the outpatient setting.

Claims (1)

METHOD FOR IDENTIFICATION OF 0-UROBILIN in a biological fluid, characterized in that, in order to reduce the time of investigation, a fluid sample is placed in a quartz cuvette and irradiated with ultraviolet light in the range 300-385 nm. and fluorescence in the red or orange region identifies 0-urobilin. '' DOF 00 ΟΪ э