SK9582002A3 - A method for detection of pathogenic organisms - Google Patents
A method for detection of pathogenic organisms Download PDFInfo
- Publication number
- SK9582002A3 SK9582002A3 SK958-2002A SK9582002A SK9582002A3 SK 9582002 A3 SK9582002 A3 SK 9582002A3 SK 9582002 A SK9582002 A SK 9582002A SK 9582002 A3 SK9582002 A3 SK 9582002A3
- Authority
- SK
- Slovakia
- Prior art keywords
- rnase
- rna
- chlamydia
- species
- gene
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 38
- 244000052769 pathogen Species 0.000 title claims abstract description 20
- 238000001514 detection method Methods 0.000 title abstract description 8
- 108090000621 Ribonuclease P Proteins 0.000 claims abstract description 81
- 102000004167 Ribonuclease P Human genes 0.000 claims abstract description 76
- 241000894007 species Species 0.000 claims abstract description 44
- 102000042567 non-coding RNA Human genes 0.000 claims abstract description 29
- 108091027963 non-coding RNA Proteins 0.000 claims abstract description 28
- 238000004458 analytical method Methods 0.000 claims abstract description 27
- 208000015181 infectious disease Diseases 0.000 claims abstract description 9
- 241000606161 Chlamydia Species 0.000 claims description 35
- 108091027305 Heteroduplex Proteins 0.000 claims description 14
- 241000186363 Mycobacterium kansasii Species 0.000 claims description 13
- 102000039446 nucleic acids Human genes 0.000 claims description 12
- 108020004707 nucleic acids Proteins 0.000 claims description 12
- 150000007523 nucleic acids Chemical class 0.000 claims description 12
- 241001502334 Mycobacterium avium complex bacterium Species 0.000 claims description 8
- 241000187485 Mycobacterium gastri Species 0.000 claims description 7
- 230000003321 amplification Effects 0.000 claims description 7
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 4
- 238000002844 melting Methods 0.000 claims description 4
- 230000008018 melting Effects 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 230000000813 microbial effect Effects 0.000 claims description 3
- 244000089742 Citrus aurantifolia Species 0.000 claims 3
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 claims 1
- 238000007796 conventional method Methods 0.000 abstract description 3
- 230000004069 differentiation Effects 0.000 abstract description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 49
- 108090000623 proteins and genes Proteins 0.000 description 31
- 101100305808 Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) rnp2 gene Proteins 0.000 description 28
- 241000606069 Chlamydiaceae Species 0.000 description 25
- 238000003752 polymerase chain reaction Methods 0.000 description 20
- 241000894006 Bacteria Species 0.000 description 19
- 239000002773 nucleotide Substances 0.000 description 18
- 125000003729 nucleotide group Chemical group 0.000 description 18
- 108020004414 DNA Proteins 0.000 description 16
- 241000186367 Mycobacterium avium Species 0.000 description 16
- 201000008827 tuberculosis Diseases 0.000 description 15
- 241000606153 Chlamydia trachomatis Species 0.000 description 14
- 241001647381 Parachlamydia acanthamoebae Species 0.000 description 13
- 241001647368 Simkania negevensis Species 0.000 description 13
- 241000282414 Homo sapiens Species 0.000 description 11
- 108020004566 Transfer RNA Proteins 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 10
- 238000012163 sequencing technique Methods 0.000 description 10
- 108020004465 16S ribosomal RNA Proteins 0.000 description 9
- 241001647378 Chlamydia psittaci Species 0.000 description 9
- 241000186364 Mycobacterium intracellulare Species 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 9
- 108010083644 Ribonucleases Proteins 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 8
- 241000498849 Chlamydiales Species 0.000 description 7
- 102000006382 Ribonucleases Human genes 0.000 description 7
- 206010000210 abortion Diseases 0.000 description 7
- 231100000176 abortion Toxicity 0.000 description 7
- 239000000499 gel Substances 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 6
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 230000003197 catalytic effect Effects 0.000 description 6
- 238000003776 cleavage reaction Methods 0.000 description 6
- 239000002243 precursor Substances 0.000 description 6
- 230000007017 scission Effects 0.000 description 6
- 238000002864 sequence alignment Methods 0.000 description 6
- 125000006850 spacer group Chemical group 0.000 description 6
- 241000282324 Felis Species 0.000 description 5
- 238000007476 Maximum Likelihood Methods 0.000 description 5
- 241000187480 Mycobacterium smegmatis Species 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 108020004513 Bacterial RNA Proteins 0.000 description 4
- 241001647373 Chlamydia abortus Species 0.000 description 4
- 241001647374 Chlamydia felis Species 0.000 description 4
- 241001647367 Chlamydia muridarum Species 0.000 description 4
- 241000192700 Cyanobacteria Species 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 241001494479 Pecora Species 0.000 description 4
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical group NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108700022487 rRNA Genes Proteins 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 3
- 208000030507 AIDS Diseases 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000271566 Aves Species 0.000 description 3
- 241001647372 Chlamydia pneumoniae Species 0.000 description 3
- 241001647370 Chlamydia suis Species 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 101710164702 Major outer membrane protein Proteins 0.000 description 3
- 241001467552 Mycobacterium bovis BCG Species 0.000 description 3
- 241000186362 Mycobacterium leprae Species 0.000 description 3
- 241000187492 Mycobacterium marinum Species 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 206010037151 Psittacosis Diseases 0.000 description 3
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- 235000015278 beef Nutrition 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 238000012512 characterization method Methods 0.000 description 3
- 238000009585 enzyme analysis Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 238000005304 joining Methods 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 201000000901 ornithosis Diseases 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 210000003705 ribosome Anatomy 0.000 description 3
- 108091029536 tRNA precursor Proteins 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- 108020005097 23S Ribosomal RNA Proteins 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 2
- 241000272525 Anas platyrhynchos Species 0.000 description 2
- 208000002109 Argyria Diseases 0.000 description 2
- 208000023514 Barrett esophagus Diseases 0.000 description 2
- 241001647371 Chlamydia caviae Species 0.000 description 2
- 241001674218 Chlamydia pecorum Species 0.000 description 2
- 241000699800 Cricetinae Species 0.000 description 2
- 239000003298 DNA probe Substances 0.000 description 2
- 241000192125 Firmicutes Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000187482 Mycobacterium avium subsp. paratuberculosis Species 0.000 description 2
- 241001134667 Mycobacterium celatum Species 0.000 description 2
- 241000186365 Mycobacterium fortuitum Species 0.000 description 2
- 241000187494 Mycobacterium xenopi Species 0.000 description 2
- 241000699700 Ondatra zibethicus Species 0.000 description 2
- 241000319342 Parachlamydiaceae Species 0.000 description 2
- 241000286209 Phasianidae Species 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 206010035673 Pneumonia chlamydial Diseases 0.000 description 2
- 241000319353 Simkaniaceae Species 0.000 description 2
- 241000589970 Spirochaetales Species 0.000 description 2
- 241000589499 Thermus thermophilus Species 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 229960000643 adenine Drugs 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 229940038705 chlamydia trachomatis Drugs 0.000 description 2
- 238000010835 comparative analysis Methods 0.000 description 2
- 229940104302 cytosine Drugs 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000002405 diagnostic procedure Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical group O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 230000007918 pathogenicity Effects 0.000 description 2
- 238000013081 phylogenetic analysis Methods 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 235000015277 pork Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000012207 quantitative assay Methods 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 102100039217 3-ketoacyl-CoA thiolase, peroxisomal Human genes 0.000 description 1
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000335423 Blastomyces Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 101100327692 Caenorhabditis elegans hsp-60 gene Proteins 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241001297667 Candidatus Wallbacteria Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 102000053642 Catalytic RNA Human genes 0.000 description 1
- 108090000994 Catalytic RNA Proteins 0.000 description 1
- 241000700199 Cavia porcellus Species 0.000 description 1
- 241000324543 Chlamydia psittaci 6BC Species 0.000 description 1
- 241001369721 Chlamydia psittaci M56 Species 0.000 description 1
- 241001290735 Chlamydia trachomatis A/HAR-13 Species 0.000 description 1
- 241000316276 Chlamydophila caviae GPIC Species 0.000 description 1
- 241000324550 Chlamydophila pecorum E58 Species 0.000 description 1
- 206010008803 Chromoblastomycosis Diseases 0.000 description 1
- 208000015116 Chromomycosis Diseases 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 241001337994 Cryptococcus <scale insect> Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 241001480035 Epidermophyton Species 0.000 description 1
- 241000620209 Escherichia coli DH5[alpha] Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000230562 Flavobacteriia Species 0.000 description 1
- 101100153048 Homo sapiens ACAA1 gene Proteins 0.000 description 1
- 101100247598 Hordeum vulgare RCAA gene Proteins 0.000 description 1
- 208000019693 Lung disease Diseases 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001074903 Methanobacteria Species 0.000 description 1
- 241001480037 Microsporum Species 0.000 description 1
- 241000235395 Mucor Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241000041810 Mycetoma Species 0.000 description 1
- 241000186359 Mycobacterium Species 0.000 description 1
- 241001467553 Mycobacterium africanum Species 0.000 description 1
- 241000187493 Mycobacterium malmoense Species 0.000 description 1
- 241000187919 Mycobacterium microti Species 0.000 description 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 1
- 241001302239 Mycobacterium tuberculosis complex Species 0.000 description 1
- 241000187644 Mycobacterium vaccae Species 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 241000283903 Ovis aries Species 0.000 description 1
- 239000008118 PEG 6000 Substances 0.000 description 1
- 241001647379 Parachlamydia Species 0.000 description 1
- 208000026681 Paratuberculosis Diseases 0.000 description 1
- 241001520299 Phascolarctos cinereus Species 0.000 description 1
- 206010036030 Polyarthritis Diseases 0.000 description 1
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
- 108010001267 Protein Subunits Proteins 0.000 description 1
- 102000002067 Protein Subunits Human genes 0.000 description 1
- 241000192142 Proteobacteria Species 0.000 description 1
- 241000235527 Rhizopus Species 0.000 description 1
- 101710089766 Ribonuclease P protein component Proteins 0.000 description 1
- 108010000605 Ribosomal Proteins Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 101710183568 Serine/threonine-protein kinase PknK Proteins 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 241001478200 Simkania Species 0.000 description 1
- 206010041736 Sporotrichosis Diseases 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 241000589596 Thermus Species 0.000 description 1
- 241000223238 Trichophyton Species 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 238000009640 blood culture Methods 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 201000001371 inclusion conjunctivitis Diseases 0.000 description 1
- 244000000056 intracellular parasite Species 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 101150024858 mpb70 gene Proteins 0.000 description 1
- 238000007857 nested PCR Methods 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 208000030428 polyarticular arthritis Diseases 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 108091092562 ribozyme Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 229910001961 silver nitrate Inorganic materials 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 206010044325 trachoma Diseases 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 230000007923 virulence factor Effects 0.000 description 1
- 239000000304 virulence factor Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Geophysics And Detection Of Objects (AREA)
- Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE0000061A SE0000061D0 (sv) | 2000-01-10 | 2000-01-10 | A method for detection of pathogenic organisms |
PCT/SE2001/000031 WO2001051662A1 (en) | 2000-01-10 | 2001-01-10 | A method for detection of pathogenic organisms |
Publications (1)
Publication Number | Publication Date |
---|---|
SK9582002A3 true SK9582002A3 (en) | 2003-01-09 |
Family
ID=20278064
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SK958-2002A SK9582002A3 (en) | 2000-01-10 | 2001-01-10 | A method for detection of pathogenic organisms |
Country Status (20)
Country | Link |
---|---|
US (2) | US8367321B2 (no) |
EP (1) | EP1254258B2 (no) |
JP (1) | JP5214083B2 (no) |
CN (1) | CN1394235A (no) |
AT (1) | ATE356884T1 (no) |
AU (1) | AU2721701A (no) |
CA (1) | CA2397176A1 (no) |
DE (1) | DE60127238T3 (no) |
EE (1) | EE200200378A (no) |
ES (1) | ES2283391T3 (no) |
HU (1) | HUP0301009A2 (no) |
IL (1) | IL150670A0 (no) |
MX (1) | MXPA02006588A (no) |
NO (1) | NO20023311L (no) |
NZ (1) | NZ519649A (no) |
PL (1) | PL356306A1 (no) |
RU (1) | RU2002121510A (no) |
SE (1) | SE0000061D0 (no) |
SK (1) | SK9582002A3 (no) |
WO (1) | WO2001051662A1 (no) |
Families Citing this family (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040121311A1 (en) | 2002-12-06 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents in livestock |
US20030027135A1 (en) | 2001-03-02 | 2003-02-06 | Ecker David J. | Method for rapid detection and identification of bioagents |
US7666588B2 (en) | 2001-03-02 | 2010-02-23 | Ibis Biosciences, Inc. | Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy |
US7226739B2 (en) | 2001-03-02 | 2007-06-05 | Isis Pharmaceuticals, Inc | Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations |
US8073627B2 (en) | 2001-06-26 | 2011-12-06 | Ibis Biosciences, Inc. | System for indentification of pathogens |
US7217510B2 (en) | 2001-06-26 | 2007-05-15 | Isis Pharmaceuticals, Inc. | Methods for providing bacterial bioagent characterizing information |
JP2006516193A (ja) | 2002-12-06 | 2006-06-29 | アイシス・ファーマシューティカルス・インコーポレーテッド | ヒトおよび動物における病原体の迅速な同定方法 |
US20040185438A1 (en) * | 2003-03-10 | 2004-09-23 | Ecker David J. | Methods of detection and notification of bioagent contamination |
US8057993B2 (en) | 2003-04-26 | 2011-11-15 | Ibis Biosciences, Inc. | Methods for identification of coronaviruses |
US8158354B2 (en) | 2003-05-13 | 2012-04-17 | Ibis Biosciences, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
US7964343B2 (en) | 2003-05-13 | 2011-06-21 | Ibis Biosciences, Inc. | Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
US8097416B2 (en) | 2003-09-11 | 2012-01-17 | Ibis Biosciences, Inc. | Methods for identification of sepsis-causing bacteria |
US8546082B2 (en) | 2003-09-11 | 2013-10-01 | Ibis Biosciences, Inc. | Methods for identification of sepsis-causing bacteria |
US20120122099A1 (en) | 2003-09-11 | 2012-05-17 | Rangarajan Sampath | Compositions for use in identification of bacteria |
US7666592B2 (en) | 2004-02-18 | 2010-02-23 | Ibis Biosciences, Inc. | Methods for concurrent identification and quantification of an unknown bioagent |
US7714275B2 (en) | 2004-05-24 | 2010-05-11 | Ibis Biosciences, Inc. | Mass spectrometry with selective ion filtration by digital thresholding |
US20050266411A1 (en) | 2004-05-25 | 2005-12-01 | Hofstadler Steven A | Methods for rapid forensic analysis of mitochondrial DNA |
US7811753B2 (en) | 2004-07-14 | 2010-10-12 | Ibis Biosciences, Inc. | Methods for repairing degraded DNA |
US7309589B2 (en) * | 2004-08-20 | 2007-12-18 | Vironix Llc | Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing |
WO2006135400A2 (en) | 2004-08-24 | 2006-12-21 | Isis Pharmaceuticals, Inc. | Methods for rapid identification of recombinant organisms |
US8084207B2 (en) | 2005-03-03 | 2011-12-27 | Ibis Bioscience, Inc. | Compositions for use in identification of papillomavirus |
EP1869180B1 (en) | 2005-03-03 | 2013-02-20 | Ibis Biosciences, Inc. | Compositions for use in identification of polyoma viruses |
CA2616281C (en) | 2005-07-21 | 2014-04-22 | Isis Pharmaceuticals, Inc. | Methods for rapid identification and quantitation of mitochondrial dna variants |
CA2663029C (en) | 2006-09-14 | 2016-07-19 | Ibis Biosciences, Inc. | Targeted whole genome amplification method for identification of pathogens |
US8871471B2 (en) | 2007-02-23 | 2014-10-28 | Ibis Biosciences, Inc. | Methods for rapid forensic DNA analysis |
US9598724B2 (en) | 2007-06-01 | 2017-03-21 | Ibis Biosciences, Inc. | Methods and compositions for multiple displacement amplification of nucleic acids |
WO2010007605A1 (en) * | 2008-07-16 | 2010-01-21 | Agriculture And Food Development Authority (Teagasc) | A method of assessing bacterial load of a sample |
US8550694B2 (en) | 2008-09-16 | 2013-10-08 | Ibis Biosciences, Inc. | Mixing cartridges, mixing stations, and related kits, systems, and methods |
US8148163B2 (en) | 2008-09-16 | 2012-04-03 | Ibis Biosciences, Inc. | Sample processing units, systems, and related methods |
EP2344893B1 (en) | 2008-09-16 | 2014-10-15 | Ibis Biosciences, Inc. | Microplate handling systems and methods |
WO2010093943A1 (en) | 2009-02-12 | 2010-08-19 | Ibis Biosciences, Inc. | Ionization probe assemblies |
US8950604B2 (en) | 2009-07-17 | 2015-02-10 | Ibis Biosciences, Inc. | Lift and mount apparatus |
US9194877B2 (en) | 2009-07-17 | 2015-11-24 | Ibis Biosciences, Inc. | Systems for bioagent indentification |
WO2011047307A1 (en) | 2009-10-15 | 2011-04-21 | Ibis Biosciences, Inc. | Multiple displacement amplification |
PL3172797T3 (pl) * | 2014-07-21 | 2021-01-11 | Telefonaktiebolaget Lm Ericsson (Publ) | Antena szczelinowa |
US20170117630A1 (en) * | 2015-10-21 | 2017-04-27 | Microsoft Technology Licensing, Llc | Single loop near field communications antenna |
JP6894439B2 (ja) * | 2016-01-04 | 2021-06-30 | ジェン−プローブ・インコーポレーテッド | Candida種を検出するための方法及び組成物 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IE81145B1 (en) * | 1989-04-20 | 2000-05-03 | Thomas Gerard Barry | Generation of specific probes for target nucleotide sequences |
US6984487B1 (en) * | 1989-08-22 | 2006-01-10 | Hsc Research Development Corporation | Cystic fibrosis gene |
JP2001514025A (ja) | 1997-09-04 | 2001-09-11 | スミスクライン・ビーチャム・コーポレイション | 新規rnasep |
US7041490B1 (en) * | 1997-11-28 | 2006-05-09 | Serono Genetics Institute, S.A. | Chlamydia trachomatis polynucleotides and vectors, recombinant host cells, DNA chips or kits containing the same |
-
2000
- 2000-01-10 SE SE0000061A patent/SE0000061D0/xx unknown
-
2001
- 2001-01-10 CN CN01803592A patent/CN1394235A/zh active Pending
- 2001-01-10 HU HU0301009A patent/HUP0301009A2/hu unknown
- 2001-01-10 US US10/169,831 patent/US8367321B2/en not_active Expired - Fee Related
- 2001-01-10 SK SK958-2002A patent/SK9582002A3/sk unknown
- 2001-01-10 ES ES01901634T patent/ES2283391T3/es not_active Expired - Lifetime
- 2001-01-10 MX MXPA02006588A patent/MXPA02006588A/es unknown
- 2001-01-10 PL PL01356306A patent/PL356306A1/xx not_active Application Discontinuation
- 2001-01-10 NZ NZ519649A patent/NZ519649A/en unknown
- 2001-01-10 CA CA002397176A patent/CA2397176A1/en not_active Abandoned
- 2001-01-10 JP JP2001551236A patent/JP5214083B2/ja not_active Expired - Fee Related
- 2001-01-10 EP EP01901634.4A patent/EP1254258B2/en not_active Expired - Lifetime
- 2001-01-10 RU RU2002121510/13A patent/RU2002121510A/ru unknown
- 2001-01-10 DE DE60127238.2T patent/DE60127238T3/de not_active Expired - Lifetime
- 2001-01-10 WO PCT/SE2001/000031 patent/WO2001051662A1/en active IP Right Grant
- 2001-01-10 AU AU27217/01A patent/AU2721701A/en not_active Abandoned
- 2001-01-10 AT AT01901634T patent/ATE356884T1/de not_active IP Right Cessation
- 2001-01-10 EE EEP200200378A patent/EE200200378A/xx unknown
- 2001-01-10 IL IL15067001A patent/IL150670A0/xx unknown
-
2002
- 2002-07-09 NO NO20023311A patent/NO20023311L/no not_active Application Discontinuation
-
2013
- 2013-01-02 US US13/732,625 patent/US20140018445A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
ES2283391T3 (es) | 2007-11-01 |
US8367321B2 (en) | 2013-02-05 |
MXPA02006588A (es) | 2004-09-10 |
SE0000061D0 (sv) | 2000-01-10 |
HUP0301009A2 (hu) | 2003-07-28 |
US20030134295A1 (en) | 2003-07-17 |
EP1254258B2 (en) | 2014-07-23 |
CA2397176A1 (en) | 2001-07-19 |
PL356306A1 (en) | 2004-06-28 |
WO2001051662A1 (en) | 2001-07-19 |
DE60127238D1 (de) | 2007-04-26 |
EP1254258A1 (en) | 2002-11-06 |
RU2002121510A (ru) | 2004-02-20 |
NO20023311L (no) | 2002-09-09 |
CN1394235A (zh) | 2003-01-29 |
EE200200378A (et) | 2003-10-15 |
DE60127238T3 (de) | 2014-12-24 |
ATE356884T1 (de) | 2007-04-15 |
EP1254258B1 (en) | 2007-03-14 |
JP5214083B2 (ja) | 2013-06-19 |
NO20023311D0 (no) | 2002-07-09 |
JP2003519494A (ja) | 2003-06-24 |
NZ519649A (en) | 2004-02-27 |
US20140018445A1 (en) | 2014-01-16 |
IL150670A0 (en) | 2003-02-12 |
AU2721701A (en) | 2001-07-24 |
DE60127238T2 (de) | 2007-12-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US8367321B2 (en) | Method for detection of pathogenic organisms | |
Liébana et al. | Assessment of genetic markers for species differentiation within the Mycobacterium tuberculosis complex | |
Ho et al. | Polymerase chain reaction for the detection of Neisseria gonorrhoeae in clinical samples. | |
JP5139620B2 (ja) | 微生物を検出する方法 | |
AU2003303307B2 (en) | Assay and compositions for detection of bacillus anthracis nucleic acid | |
Ho et al. | Comparison of Mycobacterium tuberculosis genomes reveals frequent deletions in a 20 kb variable region in clinical isolates | |
Kim et al. | Molecular analysis of isoniazid resistance in Mycobacterium tuberculosis isolates recovered from South Korea | |
Miller et al. | Polymerase chain reaction detection of Mycobacterium tuberculosis complex and Mycobacterium avium organisms in formalin-fixed tissues from culture-negative ruminants | |
Marsh et al. | Novel molecular markers of Chlamydia pecorum genetic diversity in the koala (Phascolarctos cinereus) | |
JP5902749B2 (ja) | 原核および真核生物に関する核酸プローブに基づく診断アッセイ | |
Herrmann et al. | Characterization of the rnpB gene and RNase P RNA in the order Chlamydiales. | |
Wilson | Molecular biology as a tool for taxonomy | |
Van Den Berg et al. | Coexistence of multiple PCR-ribotype strains of Clostridium difficile in faecal samples limits epidemiological studies | |
Bader et al. | Identification of Flavobacterium and Flexibacter species by species-specific polymerase chain reaction primers to the 16S ribosomal RNA gene | |
Kadra et al. | Typing of sheep clinical isolates and identification of enterotoxigenic Clostridium perfringens strains by classical methods and by polymerase chain reaction (PCR) | |
Frothingham | Evolutionary bottlenecks in the agents of tuberculosis, leprosy, and paratuberculosis | |
Wen et al. | Diversity of 16S rRNA genes of new Ehrlichia strains isolated from horses with clinical signs of Potomac horse fever | |
Godfroid et al. | Definitive differentiation between single and mixed mycobacterial infections in red deer (Cervus elaphus) by a combination of duplex amplification of p34 and f57 sequences and Hpy188I enzymatic restriction of duplex amplicons | |
Lee et al. | Group-specific PCR primers for the phylum Acidobacteria designed based on the comparative analysis of 16S rRNA gene sequences | |
US20120100545A1 (en) | Method and/or primers for the detection of mycobacterium tuberculosis | |
Ellingson et al. | Detection of Mycobacterium avium subspecies paratuberculosis in free-ranging bison (Bison bison) by PCR | |
Taylor-Robinson et al. | The biology of Mycoplasma genitalium | |
Tanner et al. | Molecular phylogeny of Chlamydia and relatives | |
Borneman et al. | Identifying microorganisms involved in specific in situ functions: experimental design considerations for rRNA gene‐based population studies and sequence‐selective PCR assays | |
Bai et al. | PCR-linked reverse DNA hybridization using oligonucleotide-specific probes of rpoB for identification of Mycobacterium avium and Mycobacterium intracellulare |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FB9A | Suspension of patent application procedure |