SI21043A - A method for isolating salinomycin from fermentation broth - Google Patents

Abstract

The present solution refers to a method of isolating salinomycin for the purpose of obtaining a granulated product. The purpose aforementioned is reached by a method for isolating salinomycin from the fermentation broth according to the invention, the nature of which consists in that the fermentation broth of salinomycin is adjusted to an acidic pH range. A suitable inert filtration aid such as diatomaceous earth, zeolites, wood flour or a mixture thereof is added in an amount of 5 to 20 wt % referred to the weight of the fermentation broth. The biomass is separated by filtration, granulated and dried in a fluid bed drier. The dry granules are ground and sieved to a required particle size.

Description

Process for isolation of salinomycin from fermentation broth

Technical area

The present invention relates to a process for isolating salinomycin from a fermentation broth obtained.

The state of the art

The spray drying of the fermentation broth, which is an anti-agglomerating agent and a filler, is used to prepare salinomycin feed grade, or isolate the pure form after biomass separation by extraction, concentration and purification of the crude product by recrystallization. Pure-form insulation is quite demanding due to the high production costs and mechanical and technological equipment of the production plant (US patent 4,212,492; US patent 4,137,247).

There is no need to prepare a pure form if salinomycin is intended for use as a veterinary drug or growth stimulant. After fermentation is complete, the treated broth can be directly dried together with the anti-agglomerating agents. Prior to drying, the slurry may be concentrated in an evaporator (US patent 4,395,491). The salinomycin fermentation broth obtained contains many by-products that cause problems after spray drying the broth. The solids agglomerate, form clumps and cannot be further processed. The main reason for the formation of agglomerates is the fat content. The fats contained in the solids show up mainly in high-temperature processing when they are moist and under mechanical loading. The product obtained from the dispenser is very dusty and therefore needs to be granulated (US patent 5,529,911).

The granulation process can be carried out either directly in a combined spray drier with a floating layer, or at the next insulation stage using a dry or wet processing method.

It has been found that it is possible to isolate granule solids from salinomycin fermentation broth without agglomerate formation.

Description of the invention

The above defects are removed by the process for isolating salinomycin from the fermentation broth in accordance with the present invention. The nature of the invention is that the fermentation broth of salinomycin is set to an acidic pH value of 4 to 5. Subsequently, a suitable inert filter aid, such as diatomaceous earth, zeolites, wood flour or mixtures thereof, is added in an amount in the range of 5 to 20 wt. % by weight of treated fermentation broth. The biomass is separated by filtration, then granulated and dried in a fluid bed dryer. The granules are then ground and sown to the required particle size.

The method in question provides a product which meets the veterinary requirements. This is appropriate because the active substance exists in the acidic pH range in the biomass cells and it is therefore possible to discharge the separate filtrate obtained in this process to a biological aerobic wastewater treatment plant. Furthermore, the dry matter content of the biomass thus obtained is greater than that of the fermentation broth, because the energy consumption required for drying the biomass is lower than the consumption for drying the total amount of fermentation broth.

Embodiments of the invention

Example 1

The fermentation broth obtained by fermentation of Streptomyces albus had a pH value of 6.1 and a salinomycin content of 18.4 g / kg after 128 hours of cultivation.

527 kg of the obtained fermentation broth was adjusted by adding 10% HCl to a pH of 4.0. The treated fermentation broths were added wood flour (8% by weight by weight of the treated fermentation broth). Using a rotary vacuum filter filled with wood flour, 119.7 kg of biomass and 460.7 kg of filtrate containing less than 0.01% salinomycin were obtained and taken to an aerobic wastewater treatment plant after neutralization. The precipitated biomass was run through a granulator and dried in a fluid bed dryer, yielding 74.2 kg of product containing 12.3% salinomycin.

Example 2

Fermentation of the fermentation broth with Streptomyces albus was complete after 120 hours. The broth had a pH value of 6.0 and a salinomycin content of 22.6 g / kg. The 589 kg fermentation broth was adjusted by adding 10% HCl to a pH of 5.0. Zeolite and diatomaceous earth (15 wt.% By weight of treated fermentation broth) were added. Using a rotary vacuum filter filled with diatomaceous earth, 184.7 kg of biomass and 495.6 kg of filtrate containing less than 0.02% salinomycin were obtained and taken as in Example 1. The decomposed biomass was passed through granulator and dried in a fluid bed dryer, yielding 106.5 kg of product containing 12.1% salinomycin.

Example 3

The fermentation broth obtained by fermentation of Streptomyces albus had a pH value of 6.2 after 124 hours of fermentation and contained 17.1 g / kg salinomycin. A pH of 7 to 8 was obtained by the addition of NaOH solution.

The fermentation broth thus treated was heated to 60 ° C and, after cooling, the pH was adjusted with dilute HCl as in Example 1. The zeolite (20 wt% by weight of fermentation broth) was added to 524 kg of treated fermentation broth. The mixture was filtered through a layer of diatomaceous earth as a filter aid using a rotary vacuum filter to obtain 184.7 kg of biomass and 495.6 kg of filtrate containing less than 0.001% salinomycin. The filtrate was removed as in Example 1. The precipitated biomass was run through a granulator and dried in a drier-dryer, yielding 80.7 kg of product containing 10.9% salinomycin.

Example 4

The fermentation broth obtained by fermentation with Streptomyces albus had a pH value of 6.3 and a salinomycin content of 15.5 g / kg after 128 hours of fermentation. The 566.6 kg fermentation broth was adjusted by adding 10% HCl to a pH of 4.7. Diatomaceous earth (5 wt.% By weight of fermentation broth) was added to the treated fermentation broths. A rotary vacuum filter filled with diatomaceous earth was used. We obtained 100.7 kg of biomass and 500.6 kg of filtrate, low in salinomycin, which after neutralization was taken to an aerobic wastewater treatment plant. The recovered biomass was then processed by granulation, dried in a fluid bed dryer, yielding 68.4 kg of dry product containing 12.8% salinomycin.

Industrial applicability

Salinomycin is used in veterinary medicine as a coccidiostat (against Coccidium) and as a growth stimulant in animal husbandry.

Claims (1)

Patent claim Process for isolating salinomycin from fermentation broth, characterized in that the fermentation broth is set to an acidic pH value of 4 to 5, suitable inert filter aids such as diatomaceous earth, zeolites, wood flour or mixtures thereof in the amount of 5 to 5 are added to the fermentation broth. 20% by weight based on the weight of the fermentation broth, the biomass is determined by filtration, granulated and then dried in a fluid bed dryer, and the obtained dry granules are ground and sown to the required particle size.