SK283167B6 - Method for isolation of salinomycin from the fermented soil - Google Patents

Abstract

Disclosed is a method for isolation of salinomycin, wherein the granulated product is obtained. The isolation consists in that the fermented soil of salinomycin is modified into the acid area of pH. To the modified fermented soil the inert filtration agent is added, preferably diatomaceous earth, zeolites, woody powder, respectively their mixture, in the amount of 5 to 20 % per volume of fermented soil. The biomass is separated by filtration and goes through the granulation into the fluid stove, the obtained product is ground and sifted.

Description

Technical field

The invention relates to a process for the isolation of salinomycin from fermented soil.

BACKGROUND OF THE INVENTION

Salinomycin feed batch is prepared by spray-drying the fermented soils together with an anti-caking agent and a filler or isolated to a pure form after separation of the biomass by extraction, concentration and purification by recrystallization of the crude product. Insulation to pure mold is demanding on manufacturing costs and plant engineering equipment (U.S. Pat. No. 4,212,942, U.S. Pat. No. 4 * 137,247).

It is not necessary to prepare a pure form for the use of salinomycin as a veterinary drug or as a growth promoter. After fermentation and treatment, the soil can be directly dried together with anti-caking agents, and can be concentrated by evaporation before drying (US Pat. No. 4,395,491).

The fermented soil of salinomycin contains a number of ballast substances which cause problems after drying in a spray drier. Solids aggregate, forming lumps that can no longer be processed. The main cause of agglomerate formation is the fat content. The fats contained in solids are particularly evident when processed at high temperatures, humidity and mechanical stress. The spray dryer product is very dusty and needs to be granulated (U.S. Pat. No. 5,529,911). The granulation can be carried out directly in a combined fluidized bed spray drier or as a further isolation step by dry or wet process.

It has been found that a granular solid can be isolated from salinomycin fermented soil without agglomeration.

SUMMARY OF THE INVENTION

The above mentioned disadvantages are eliminated by the process of isolation of salinomycin from the fermentation broth according to the invention, which consists in that the fermented broth of salinomycin is adjusted to an acid pH range of 4 to 5. An inert filtering agent, preferably diatomaceous earth, zeolites, woody the flour or mixture thereof in an amount of 5 to 20% by weight of the fermented soil. The biomass is collected by filtration and passed through granulation to a fluid bed drier, the product obtained is ground and sieved.

This process provides a product that does not agglomerate the particles. The advantage is that the active substance is in the acidic pH range of the biomass cells, so that the separated filtrate obtained by this process can be discharged for aerobic treatment to a biological waste water treatment plant. A further advantage is that the biomass obtained has a higher dry matter than fermented soil and thus requires less energy to dry than the entire fermented soil.

DETAILED DESCRIPTION OF THE INVENTION

Example 1

The fermentation broth obtained by fermentation of Streptomyces albus had after 128 hours. cultivation pH 6.1 and salinomycin content 18.4 g / kg. 527 kg of fermented soil was adjusted to pH 4.0 by addition of 10% HCl. Wood flour was added to the treated fermented soil in an amount of 8% by weight of the fermented soil. Filtration on a rotary vacuum filter packed with wood meal gave 119.7 kg of biomass and 460.7 kg of a filter here with a salinomycin content below 0.01%, which after neutralization continued for aerobic wastewater treatment. The separated biomass was passed through a granulator to a fluid-bed dryer where 74.2 kg of product containing 12.3% salinomycin were dried.

Example 2

The fermentation broth obtained by fermentation of Streptomyces albus had after 120 hours. cultivation pH 6.0 and salinomycin content 22.6 g / kg. 589 kg of fermented soil was adjusted to pH 5.0 by addition of 10% HCl. Zeolite and diatomaceous earth were added to the treated fermented soil in an amount of 15% by weight of the fermented soil. Filtration on a rotary vacuum filter packed with diatomaceous earth yielded 184.7 kg of biomass and 495.6 kg of a filtrate with salinomycin content below 0.02%, which was discarded as in Example 1. The separated biomass was passed through a granulator to a fluid-bed dryer where drying was obtained 106.5 kg of product containing 12.1% salinomycin.

Example 3

The fermentation broth obtained by fermentation of Streptomyces albus had after 124 hours. cultivation pH 6.2 and salinomycin content 17.1 g / kg. The pH was adjusted to 7-8 by the addition of NaOH solution. The fermented soil thus treated was driven to 60 ° C and after cooling the pH was adjusted with dilute hydrochloric acid as in Example 1. Zeolite was added to 524 kg of treated fermented soil. 20% by weight of fermented soil. Filtration on a rotary vacuum filter packed with diatomaceous earth yielded 184.7 kg of biomass and 495.6 kg of a filtrate with a salinomycin content below 0.001%. Disposal of the filtrate as in Example L The separated biomass was passed through a granulator to a fluidized bed dryer where drying was obtained.

80.7 kg of the product containing 10.9% salinomycin.

Example 4

The fermentation broth obtained by fermentation of Streptomyces albus had after 120 hours. cultivation pH 6.3 and salinomycin content 15.5 g / kg. 566.6 kg of fermented soil was adjusted to pH 4.7 by addition of 10% HCl. Kieselguhr was added to the treated fermented soil in an amount of 5% by weight of the fermented soil. Filtration on a kieselguhr rotary vacuum filter yielded

100.7 kg of biomass. 500.6 kg of low salinomycin filtrate, which after neutralization continued for aerobic waste water treatment. The separated biomass was passed through a granulator to a fluidized bed dryer, where 68.4 kg of dry product were dried. The salinomycin content of the product is 12.8%.

Industrial usability

Salinomycin is used as an anticoccidial drug in veterinary medicine and as a growth promoter in livestock nutrition.

Claims (1)

PATENT CLAIMS Process for the isolation of salinomycin from fermented soil, characterized in that the fermented soil is brought to an acidic pH range of 4 to 5, further an inert filter medium, preferably diatomaceous earth, zeolites, wood meal or a mixture thereof, is added to the treated fermented soil. 5 to 20% by volume of the fermented soil, the biomass is separated by filtration and the biomass thus separated is passed through granulation to a fluid-bed dryer, the product obtained is ground and sieved.