SG193437A1 - Scalable spectroscopic detection and measurement - Google Patents

Scalable spectroscopic detection and measurement Download PDF

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Publication number
SG193437A1
SG193437A1 SG2013068820A SG2013068820A SG193437A1 SG 193437 A1 SG193437 A1 SG 193437A1 SG 2013068820 A SG2013068820 A SG 2013068820A SG 2013068820 A SG2013068820 A SG 2013068820A SG 193437 A1 SG193437 A1 SG 193437A1
Authority
SG
Singapore
Prior art keywords
light
sample
image sensor
light source
detection
Prior art date
Application number
SG2013068820A
Other languages
English (en)
Inventor
David Tracy
Peter Lewis Stokes
Sepehr Kiani
Original Assignee
Gnubio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gnubio Inc filed Critical Gnubio Inc
Publication of SG193437A1 publication Critical patent/SG193437A1/en

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Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28Investigating the spectrum
    • G01J3/42Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02Details
    • G01J3/0205Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/0208Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02Details
    • G01J3/0205Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/021Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using plane or convex mirrors, parallel phase plates, or particular reflectors
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02Details
    • G01J3/0205Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/0216Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using light concentrators or collectors or condensers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02Details
    • G01J3/0205Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/0248Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using a sighting port, e.g. camera or human eye
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02Details
    • G01J3/0291Housings; Spectrometer accessories; Spatial arrangement of elements, e.g. folded path arrangements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02Details
    • G01J3/06Scanning arrangements arrangements for order-selection
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02Details
    • G01J3/08Beam switching arrangements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02Details
    • G01J3/10Arrangements of light sources specially adapted for spectrometry or colorimetry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12Generating the spectrum; Monochromators
    • G01J3/14Generating the spectrum; Monochromators using refracting elements, e.g. prisms
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12Generating the spectrum; Monochromators
    • G01J3/18Generating the spectrum; Monochromators using diffraction elements, e.g. grating
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28Investigating the spectrum
    • G01J3/2803Investigating the spectrum using photoelectric array detector
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28Investigating the spectrum
    • G01J3/44Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28Investigating the spectrum
    • G01J3/44Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406Fluorescence spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434Optical arrangements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484Optical investigation techniques, e.g. flow cytometry microstructural devices
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6456Spatial resolved fluorescence measurements; Imaging

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  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Dispersion Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
SG2013068820A 2011-03-31 2012-03-27 Scalable spectroscopic detection and measurement SG193437A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161469889P 2011-03-31 2011-03-31
PCT/US2012/030716 WO2012135201A1 (en) 2011-03-31 2012-03-27 Scalable spectroscopic detection and measurement

Publications (1)

Publication Number Publication Date
SG193437A1 true SG193437A1 (en) 2013-10-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
SG2013068820A SG193437A1 (en) 2011-03-31 2012-03-27 Scalable spectroscopic detection and measurement

Country Status (8)

Country Link
US (1) US9228898B2 (enExample)
EP (1) EP2691752A4 (enExample)
JP (1) JP5986623B2 (enExample)
CN (1) CN103582805B (enExample)
AU (1) AU2012236748B2 (enExample)
CA (1) CA2841425C (enExample)
SG (1) SG193437A1 (enExample)
WO (1) WO2012135201A1 (enExample)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2820094C (en) 2010-12-07 2019-02-26 Gnubio, Inc. Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides
JP6472998B2 (ja) 2011-03-30 2019-02-20 バイオ−ラッド ラボラトリーズ インコーポレイテッド 液滴内への、または液滴からの複数の容量の注入
WO2012135201A1 (en) 2011-03-31 2012-10-04 Gnubio, Inc. Scalable spectroscopic detection and measurement
EP2691540B1 (en) 2011-03-31 2016-01-20 GnuBIO, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
US9821312B2 (en) 2012-09-12 2017-11-21 Bio-Rad Laboratories, Inc. Integrated microfluidic system, method and kit for performing assays
US9291503B2 (en) * 2012-11-08 2016-03-22 Hitachi, Ltd. Flow type single-particle spectrometer
US9592503B2 (en) 2013-01-25 2017-03-14 Gnubio, Inc. System and method for performing droplet inflation
EP3004813A4 (en) 2013-05-29 2016-12-21 Gnubio Inc COST-EFFECTIVE OPTICAL DISCRETE HIGH-SPEED MEASURING SYSTEM
EP3004391B1 (en) 2013-05-29 2019-03-27 Bio-Rad Laboratories, Inc. Methods for sequencing in emulsion based microfluidics
CN105765055A (zh) 2013-08-27 2016-07-13 基纽拜奥股份有限公司 微流体装置和其使用方法
EP3052236B1 (en) 2013-09-30 2021-07-14 Bio-Rad Laboratories, Inc. Microfluidic cartridge device and methods of use and assembly
US10130950B2 (en) 2013-11-27 2018-11-20 Bio-Rad Laboratories, Inc. Microfluidic droplet packing
US9869664B2 (en) * 2013-12-18 2018-01-16 Saudi Arabian Oil Company Method for non-intrusive measurement of low water content in oil
US9683792B2 (en) 2014-06-30 2017-06-20 Bio-Rad Laboratories, Inc. Floating thermal contact enabled PCR
GB201415238D0 (en) * 2014-08-28 2014-10-15 Renishaw Plc Spectroscopy apparatus
TWI544210B (zh) * 2014-10-21 2016-08-01 王基旆 可攜式電子裝置、結合該裝置之光譜儀及利用該裝置檢測物品品質之方法
US10215771B2 (en) 2014-11-06 2019-02-26 Bio-Rad Laboratories, Inc. Droplet velocity detection
CN104483104B (zh) * 2014-12-25 2018-01-12 中国科学院半导体研究所 一种光电探测器光谱响应分析系统
KR20240116531A (ko) 2015-02-09 2024-07-29 슬링샷 바이오사이언시즈 인코포레이티드 튜닝가능한 광 특성을 갖는 하이드로겔 입자 및 이를 사용하기 위한 방법
CN107250762B (zh) * 2015-03-06 2020-06-30 贝克顿·迪金森公司 集光系统及其制造和使用方法
CN107923070A (zh) 2015-08-25 2018-04-17 生物辐射实验室股份有限公司 数字式免疫测定
US10386284B2 (en) * 2016-01-29 2019-08-20 JianFeng Zhang Device and method for measurement of dispersed objects using fluorescent and non-fluorescent imaging with laser
WO2017180699A1 (en) 2016-04-14 2017-10-19 Saudi Arabian Oil Company Characterizing petroleum product contamination using fluorescence signal
WO2017180704A1 (en) 2016-04-14 2017-10-19 Saudi Arabian Oil Company Opto-mechanical part for parabolic mirror fine rotation and on-axis linear positioning
WO2017189603A1 (en) 2016-04-26 2017-11-02 Saudi Arabian Oil Company Characterizing lubricant oil degradation using fluorescence signals
CN109557015B (zh) * 2016-11-01 2021-05-25 北京信息科技大学 流式细胞仪光谱重叠信号数字处理方法
JP6784603B2 (ja) * 2017-01-05 2020-11-11 東レエンジニアリング株式会社 分光測定方法および分光測定装置
EP3747985A4 (en) * 2018-02-01 2021-10-27 Agc Inc. CELL CULTURE CONTAINER
CN111742212B (zh) 2019-01-08 2024-02-20 京东方科技集团股份有限公司 流体检测面板和流体检测装置
CN109632660B (zh) * 2019-01-17 2022-04-05 京东方科技集团股份有限公司 流体检测面板
CN114829626A (zh) 2019-10-10 2022-07-29 1859公司 用于微流体筛选的方法和系统
EP4094064A4 (en) 2020-01-24 2024-02-28 Slingshot Biosciences, Inc. Compositions and methods for cell-like calibration particles
KR20230029612A (ko) 2020-05-04 2023-03-03 슬링샷 바이오사이언시즈 인코포레이티드 다중 검정을 위한 수동 광학 바코딩용 조성물 및 방법
WO2022140291A1 (en) * 2020-12-21 2022-06-30 Singular Genomics Systems, Inc. Systems and methods for multicolor imaging
CN113155287B (zh) * 2021-04-27 2022-10-21 江苏汇鼎光学眼镜有限公司 一种眼镜片测色装置
CA3235582A1 (en) 2021-10-29 2023-05-04 Anh Tuan Nguyen Hydrogel particles as feeder cells and as synthetic antigen presenting cells
US12259377B2 (en) 2022-04-05 2025-03-25 Saudi Arabian Oil Company Detecting hydrocarbon fuels in lubrication oils
EP4519690A1 (en) 2022-05-05 2025-03-12 Slingshot Biosciences, Inc. Engineered particles as red blood cell mimics and compositions containing same for hematology
US12216041B2 (en) 2022-05-31 2025-02-04 Saudi Arabian Oil Company Method to detect diesel in fluid samples using partially dissolvable cuvettes
KR20250025383A (ko) 2022-06-02 2025-02-21 슬링샷 바이오사이언시즈 인코포레이티드 아폽토시스 세포 모방체
CN120712108A (zh) 2022-10-26 2025-09-26 弹弓生物科学公司 具有可调整光学性质的大小可调整合成颗粒和用于使用其进行免疫细胞激活的方法
US12298266B2 (en) 2023-03-30 2025-05-13 Saudi Arabian Oil Company Changes in resistivity of mesh-patterned polymer thin films coated with conductive traces used to detect hydrocarbon fuels hidden in paraffin-based lubrication oils
WO2025049609A1 (en) 2023-08-29 2025-03-06 Slingshot Biosciences, Inc. Cd34 stem cell mimics
DE102023131464A1 (de) * 2023-11-13 2025-05-15 Fluidect Gmbh Detektionssystem und Verfahren zum Erfassen einer elektromagnetischen Emission von Mikropartikeln

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2975669A (en) 1954-08-26 1961-03-21 Jarrell Ash Company Crossed dispersion photographic spectrometer
US5159199A (en) 1991-08-12 1992-10-27 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Integrated filter and detector array for spectral imaging
US5206701A (en) * 1991-09-20 1993-04-27 Amoco Corporation Apparatus for near-infrared spectrophotometric analysis
US5434629A (en) 1993-12-20 1995-07-18 Focus Automation Systems Inc. Real-time line scan processor
US5610771A (en) 1994-08-01 1997-03-11 Lockheed Missiles & Space Company, Inc. Non-deviating prism with continuously variable dispersion
US6017434A (en) 1995-05-09 2000-01-25 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US6982431B2 (en) * 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
US6043881A (en) * 1998-03-06 2000-03-28 Praxair Technology Inc Sample cell for gaseous emission spectroscopy
US7079241B2 (en) 2000-04-06 2006-07-18 Invitrogen Corp. Spatial positioning of spectrally labeled beads
US6975400B2 (en) * 1999-01-25 2005-12-13 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
US6875973B2 (en) * 2000-08-25 2005-04-05 Amnis Corporation Auto focus for a flow imaging system
WO2002101339A2 (en) * 2000-10-12 2002-12-19 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
US6856390B2 (en) * 2001-07-25 2005-02-15 Applera Corporation Time-delay integration in electrophoretic detection systems
US6831688B2 (en) * 2002-04-08 2004-12-14 Recon/Optical, Inc. Multispectral or hyperspectral imaging system and method for tactical reconnaissance
JP2006520900A (ja) 2003-03-11 2006-09-14 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 励起システム及び焦点モニタリングシステムを備える分光分析装置及び方法
EP2662136A3 (en) 2003-08-27 2013-12-25 President and Fellows of Harvard College Method for handling and mixing droplets
CA2504817A1 (en) 2004-04-21 2005-10-21 University Technologies International Inc. Synchronization of time delay and integration cameras
US7046359B2 (en) 2004-06-30 2006-05-16 Chemimage Corporation System and method for dynamic chemical imaging
JP4982385B2 (ja) * 2005-02-01 2012-07-25 アムニス コーポレイション イメージングフローサイトメータを使用した血液及び細胞の分析
WO2006110135A1 (en) 2005-04-08 2006-10-19 Chemimage Corporation System and method for chemical imaging of microarrays
EP3913375A1 (en) 2006-01-11 2021-11-24 Bio-Rad Laboratories, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US7815871B2 (en) * 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet microactuator system
EP2021113A2 (en) 2006-05-11 2009-02-11 Raindance Technologies, Inc. Microfluidic devices
GB0611981D0 (en) 2006-06-16 2006-07-26 Renishaw Plc Spectroscopic analysis methods
US8179526B2 (en) * 2007-01-25 2012-05-15 Renishaw Plc Spectroscopic apparatus with dispersive device for collecting sample data in synchronism with relative movement of a focus
US9797010B2 (en) 2007-12-21 2017-10-24 President And Fellows Of Harvard College Systems and methods for nucleic acid sequencing
EP2286125B1 (en) 2008-05-16 2015-07-08 President and Fellows of Harvard College Valves and other flow control in fluidic systems including microfluidic systems
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3290531B1 (en) 2008-12-19 2019-07-24 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
US8047829B1 (en) 2009-01-26 2011-11-01 Sandia Corporation Method for forming polymerized microfluidic devices
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
JP5841937B2 (ja) 2009-06-26 2016-01-13 プレジデント アンド フェローズ オブ ハーバード カレッジ 流体注入
EP2493619B1 (en) 2009-10-27 2018-12-19 President and Fellows of Harvard College Droplet creation techniques
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
CA2820094C (en) 2010-12-07 2019-02-26 Gnubio, Inc. Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
JP6472998B2 (ja) 2011-03-30 2019-02-20 バイオ−ラッド ラボラトリーズ インコーポレイテッド 液滴内への、または液滴からの複数の容量の注入
WO2012135201A1 (en) 2011-03-31 2012-10-04 Gnubio, Inc. Scalable spectroscopic detection and measurement
EP2691540B1 (en) 2011-03-31 2016-01-20 GnuBIO, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US11389800B2 (en) 2011-09-28 2022-07-19 President And Fellows Of Harvard College Systems and methods for droplet production and/or fluidic manipulation
US20150045258A1 (en) 2012-02-14 2015-02-12 Gnubio, Inc. Cascaded addition of target specific universal adapters to nucleic acids
WO2013165748A1 (en) 2012-04-30 2013-11-07 Raindance Technologies, Inc Digital analyte analysis
US9821312B2 (en) 2012-09-12 2017-11-21 Bio-Rad Laboratories, Inc. Integrated microfluidic system, method and kit for performing assays
US20150321163A1 (en) 2012-12-14 2015-11-12 Gnubio, Inc. Method for maintaining heterogeneous concentrations of molecules in emulsion droplets
US9592503B2 (en) 2013-01-25 2017-03-14 Gnubio, Inc. System and method for performing droplet inflation
EP2989213B1 (en) 2013-04-26 2019-11-06 Bio-Rad Laboratories, Inc. A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure
EP3004391B1 (en) 2013-05-29 2019-03-27 Bio-Rad Laboratories, Inc. Methods for sequencing in emulsion based microfluidics

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CN103582805B (zh) 2016-08-10
CA2841425C (en) 2018-05-01
US20140198313A1 (en) 2014-07-17
CA2841425A1 (en) 2012-10-04
JP5986623B2 (ja) 2016-09-06
US9228898B2 (en) 2016-01-05
CN103582805A (zh) 2014-02-12
AU2012236748A1 (en) 2013-10-03
AU2012236748B2 (en) 2015-11-26
JP2014512532A (ja) 2014-05-22
EP2691752A1 (en) 2014-02-05

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