SG190076A1 - Analysis of fragmented genomic dna in droplets - Google Patents
Analysis of fragmented genomic dna in droplets Download PDFInfo
- Publication number
- SG190076A1 SG190076A1 SG2013033238A SG2013033238A SG190076A1 SG 190076 A1 SG190076 A1 SG 190076A1 SG 2013033238 A SG2013033238 A SG 2013033238A SG 2013033238 A SG2013033238 A SG 2013033238A SG 190076 A1 SG190076 A1 SG 190076A1
- Authority
- SG
- Singapore
- Prior art keywords
- droplets
- dna
- genomic dna
- per
- droplet
- Prior art date
Links
- 238000004458 analytical method Methods 0.000 title description 4
- 238000000034 method Methods 0.000 claims abstract description 70
- 238000003556 assay Methods 0.000 claims abstract description 24
- 108091008146 restriction endonucleases Proteins 0.000 claims description 11
- 238000000638 solvent extraction Methods 0.000 claims description 4
- 238000010008 shearing Methods 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 52
- 241000282414 Homo sapiens Species 0.000 description 8
- 239000012071 phase Substances 0.000 description 7
- 238000013467 fragmentation Methods 0.000 description 6
- 238000006062 fragmentation reaction Methods 0.000 description 6
- 239000000839 emulsion Substances 0.000 description 5
- 238000003752 polymerase chain reaction Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000013459 approach Methods 0.000 description 4
- 239000013068 control sample Substances 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 239000012634 fragment Substances 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000008346 aqueous phase Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000012203 high throughput assay Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000004581 coalescence Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 239000007762 w/o emulsion Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US40910610P | 2010-11-01 | 2010-11-01 | |
| US12/976,827 US9598725B2 (en) | 2010-03-02 | 2010-12-22 | Emulsion chemistry for encapsulated droplets |
| PCT/US2011/058854 WO2012061442A1 (en) | 2010-11-01 | 2011-11-01 | Analysis of fragmented genomic dna in droplets |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG190076A1 true SG190076A1 (en) | 2013-06-28 |
Family
ID=46024812
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG2013033238A SG190076A1 (en) | 2010-11-01 | 2011-11-01 | Analysis of fragmented genomic dna in droplets |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP2635708A4 (https=) |
| JP (1) | JP2013541347A (https=) |
| CN (1) | CN103443289A (https=) |
| AU (1) | AU2011323507A1 (https=) |
| CA (1) | CA2816702A1 (https=) |
| SG (1) | SG190076A1 (https=) |
| WO (1) | WO2012061442A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11123740B2 (en) | 2015-06-29 | 2021-09-21 | Arizona Board Of Regents On Behalf Of Arizona State University | Systems and methods for continuous flow digital droplet polymerase chain reaction bioanalysis |
| EP3339447B1 (en) | 2015-08-28 | 2020-07-29 | Kabushiki Kaisha DNAFORM | Method for analyzing template nucleic acid, method for analyzing target substance, analysis kit for template nucleic acid or target substance, and analyzer for template nucleic acid or target substance |
| WO2018098438A1 (en) | 2016-11-28 | 2018-05-31 | Arizona Board Of Regents On Behalf Of Arizona State University | Systems and methods related to continuous flow droplet reaction |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5555191A (en) * | 1994-10-12 | 1996-09-10 | Trustees Of Columbia University In The City Of New York | Automated statistical tracker |
| WO2005043154A2 (en) * | 2003-10-27 | 2005-05-12 | Massachusetts Institute Of Technology | High density reaction chambers and methods of use |
| JP2009536313A (ja) * | 2006-01-11 | 2009-10-08 | レインダンス テクノロジーズ， インコーポレイテッド | ナノリアクターの形成および制御において使用するマイクロ流体デバイスおよび方法 |
| US8383345B2 (en) * | 2008-09-12 | 2013-02-26 | University Of Washington | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
| US9156010B2 (en) * | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
-
2011
- 2011-11-01 WO PCT/US2011/058854 patent/WO2012061442A1/en not_active Ceased
- 2011-11-01 JP JP2013537769A patent/JP2013541347A/ja active Pending
- 2011-11-01 EP EP11838712.5A patent/EP2635708A4/en not_active Withdrawn
- 2011-11-01 CA CA2816702A patent/CA2816702A1/en not_active Abandoned
- 2011-11-01 AU AU2011323507A patent/AU2011323507A1/en not_active Abandoned
- 2011-11-01 SG SG2013033238A patent/SG190076A1/en unknown
- 2011-11-01 CN CN2011800528318A patent/CN103443289A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012061442A1 (en) | 2012-05-10 |
| AU2011323507A1 (en) | 2013-06-13 |
| CN103443289A (zh) | 2013-12-11 |
| JP2013541347A (ja) | 2013-11-14 |
| EP2635708A1 (en) | 2013-09-11 |
| CA2816702A1 (en) | 2012-05-10 |
| EP2635708A4 (en) | 2014-10-29 |
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