CN103443289A - 液滴中的碎片化的基因组dna的分析 - Google Patents
液滴中的碎片化的基因组dna的分析 Download PDFInfo
- Publication number
- CN103443289A CN103443289A CN2011800528318A CN201180052831A CN103443289A CN 103443289 A CN103443289 A CN 103443289A CN 2011800528318 A CN2011800528318 A CN 2011800528318A CN 201180052831 A CN201180052831 A CN 201180052831A CN 103443289 A CN103443289 A CN 103443289A
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- Prior art keywords
- drop
- dna
- fragmentation
- genomic dna
- approximately
- Prior art date
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- Pending
Links
- 238000004458 analytical method Methods 0.000 title description 3
- 238000000034 method Methods 0.000 claims abstract description 76
- 238000013467 fragmentation Methods 0.000 claims description 60
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- 108091008146 restriction endonucleases Proteins 0.000 claims description 10
- 108090000623 proteins and genes Proteins 0.000 claims description 6
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US40910610P | 2010-11-01 | 2010-11-01 | |
| US61/409,106 | 2010-11-01 | ||
| US12/976,827 US9598725B2 (en) | 2010-03-02 | 2010-12-22 | Emulsion chemistry for encapsulated droplets |
| US12/976,827 | 2010-12-22 | ||
| PCT/US2011/058854 WO2012061442A1 (en) | 2010-11-01 | 2011-11-01 | Analysis of fragmented genomic dna in droplets |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103443289A true CN103443289A (zh) | 2013-12-11 |
Family
ID=46024812
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011800528318A Pending CN103443289A (zh) | 2010-11-01 | 2011-11-01 | 液滴中的碎片化的基因组dna的分析 |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP2635708A4 (https=) |
| JP (1) | JP2013541347A (https=) |
| CN (1) | CN103443289A (https=) |
| AU (1) | AU2011323507A1 (https=) |
| CA (1) | CA2816702A1 (https=) |
| SG (1) | SG190076A1 (https=) |
| WO (1) | WO2012061442A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11123740B2 (en) | 2015-06-29 | 2021-09-21 | Arizona Board Of Regents On Behalf Of Arizona State University | Systems and methods for continuous flow digital droplet polymerase chain reaction bioanalysis |
| EP3339447B1 (en) | 2015-08-28 | 2020-07-29 | Kabushiki Kaisha DNAFORM | Method for analyzing template nucleic acid, method for analyzing target substance, analysis kit for template nucleic acid or target substance, and analyzer for template nucleic acid or target substance |
| WO2018098438A1 (en) | 2016-11-28 | 2018-05-31 | Arizona Board Of Regents On Behalf Of Arizona State University | Systems and methods related to continuous flow droplet reaction |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5555191A (en) * | 1994-10-12 | 1996-09-10 | Trustees Of Columbia University In The City Of New York | Automated statistical tracker |
| US20050277125A1 (en) * | 2003-10-27 | 2005-12-15 | Massachusetts Institute Of Technology | High-density reaction chambers and methods of use |
| US20100069263A1 (en) * | 2008-09-12 | 2010-03-18 | Washington, University Of | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
| US20100137163A1 (en) * | 2006-01-11 | 2010-06-03 | Link Darren R | Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9156010B2 (en) * | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
-
2011
- 2011-11-01 WO PCT/US2011/058854 patent/WO2012061442A1/en not_active Ceased
- 2011-11-01 JP JP2013537769A patent/JP2013541347A/ja active Pending
- 2011-11-01 EP EP11838712.5A patent/EP2635708A4/en not_active Withdrawn
- 2011-11-01 CA CA2816702A patent/CA2816702A1/en not_active Abandoned
- 2011-11-01 AU AU2011323507A patent/AU2011323507A1/en not_active Abandoned
- 2011-11-01 SG SG2013033238A patent/SG190076A1/en unknown
- 2011-11-01 CN CN2011800528318A patent/CN103443289A/zh active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5555191A (en) * | 1994-10-12 | 1996-09-10 | Trustees Of Columbia University In The City Of New York | Automated statistical tracker |
| US20050277125A1 (en) * | 2003-10-27 | 2005-12-15 | Massachusetts Institute Of Technology | High-density reaction chambers and methods of use |
| US20100137163A1 (en) * | 2006-01-11 | 2010-06-03 | Link Darren R | Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors |
| US20100069263A1 (en) * | 2008-09-12 | 2010-03-18 | Washington, University Of | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012061442A1 (en) | 2012-05-10 |
| AU2011323507A1 (en) | 2013-06-13 |
| SG190076A1 (en) | 2013-06-28 |
| JP2013541347A (ja) | 2013-11-14 |
| EP2635708A1 (en) | 2013-09-11 |
| CA2816702A1 (en) | 2012-05-10 |
| EP2635708A4 (en) | 2014-10-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C05 | Deemed withdrawal (patent law before 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20131211 |