SG181152A1 - Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose - Google Patents

Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose Download PDF

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Publication number
SG181152A1
SG181152A1 SG2012040002A SG2012040002A SG181152A1 SG 181152 A1 SG181152 A1 SG 181152A1 SG 2012040002 A SG2012040002 A SG 2012040002A SG 2012040002 A SG2012040002 A SG 2012040002A SG 181152 A1 SG181152 A1 SG 181152A1
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SG
Singapore
Prior art keywords
seq
sucrose
glycerol
set forth
polypeptide
Prior art date
Application number
SG2012040002A
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English (en)
Inventor
Andrew C Eliot
Anthony A Gatenby
Dyk Tina K Van
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of SG181152A1 publication Critical patent/SG181152A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2451Glucanases acting on alpha-1,6-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20Glycerol
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42Hydroxy-carboxylic acids

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SG2012040002A 2009-12-04 2010-12-03 Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose SG181152A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26660509P 2009-12-04 2009-12-04
PCT/US2010/058832 WO2011069033A1 (en) 2009-12-04 2010-12-03 Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose

Publications (1)

Publication Number Publication Date
SG181152A1 true SG181152A1 (en) 2012-07-30

Family

ID=43499925

Family Applications (1)

Application Number Title Priority Date Filing Date
SG2012040002A SG181152A1 (en) 2009-12-04 2010-12-03 Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose

Country Status (10)

Country Link
US (1) US20110136190A1 (cg-RX-API-DMAC7.html)
EP (1) EP2507377A1 (cg-RX-API-DMAC7.html)
JP (1) JP2013512675A (cg-RX-API-DMAC7.html)
KR (1) KR20120099116A (cg-RX-API-DMAC7.html)
CN (1) CN103080326A (cg-RX-API-DMAC7.html)
AU (1) AU2010325895B2 (cg-RX-API-DMAC7.html)
BR (1) BR112012012719A2 (cg-RX-API-DMAC7.html)
CA (1) CA2780757A1 (cg-RX-API-DMAC7.html)
SG (1) SG181152A1 (cg-RX-API-DMAC7.html)
WO (1) WO2011069033A1 (cg-RX-API-DMAC7.html)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8129170B1 (en) * 2010-12-06 2012-03-06 E.I. Du Pont De Nemours And Company Recombinant bacteria having the ability to metabolize sucrose
US8673602B2 (en) 2011-08-16 2014-03-18 E I Du Pont De Nemours And Company Recombinant bacteria having improved sucrose utilization
US8629243B2 (en) 2011-08-16 2014-01-14 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria
US8686114B2 (en) * 2012-03-05 2014-04-01 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides
US9017961B2 (en) * 2012-03-05 2015-04-28 E.I. Du Pont De Nemours And Company Recombinant bacteria comprising novel sucrose transporters

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5686276A (en) 1995-05-12 1997-11-11 E. I. Du Pont De Nemours And Company Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism
BR9712942B1 (pt) * 1996-11-13 2010-09-21 método para a produção de glicerol e célula hospedeira de e. coli transformada.
CA2269088C (en) * 1996-11-13 2009-07-28 E.I. Du Pont De Nemours And Company Method for the production of 1,3-propanediol by recombinant organisms
CN1202253C (zh) 1997-12-02 2005-05-18 纳幕尔杜邦公司 用重组生物体生产甘油的方法
DE60029971T2 (de) 1999-08-18 2007-05-16 E.I. Dupont De Nemours And Co., Wilmington Verfahren zur biologischen herstellung von 1,3-propandiol
RU2212447C2 (ru) * 2000-04-26 2003-09-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Штамм escherichia coli - продуцент аминокислоты (варианты) и способ получения аминокислот (варианты)
CA2481414C (en) 2002-04-22 2013-04-09 E.I. Du Pont De Nemours And Company Promoter and plasmid system for genetic engineering
BR0314498A (pt) 2002-10-04 2005-08-16 Du Pont Linhagens de e. coli e método para a bioprodução de 1,3-propanodiol
JP5041570B2 (ja) * 2002-10-04 2012-10-03 ジェネンコー・インターナショナル・インク 生物資源の生産に対するグルコース輸送変異体
US7524660B2 (en) * 2005-05-05 2009-04-28 E.I. Du Pont De Nemours And Company Utilization of fructose in microbial production strains
TWI521062B (zh) * 2007-03-23 2016-02-11 代謝探索公司 製造1,2-丙二醇及丙酮醇之微生物及方法
WO2010051849A1 (en) * 2008-11-07 2010-05-14 Metabolic Explorer Use of sucrose as substrate for fermentative production of 1,2-propanediol

Also Published As

Publication number Publication date
US20110136190A1 (en) 2011-06-09
KR20120099116A (ko) 2012-09-06
WO2011069033A1 (en) 2011-06-09
BR112012012719A2 (pt) 2015-09-29
AU2010325895A1 (en) 2012-05-31
CN103080326A (zh) 2013-05-01
EP2507377A1 (en) 2012-10-10
JP2013512675A (ja) 2013-04-18
CA2780757A1 (en) 2011-06-09
AU2010325895B2 (en) 2014-11-06

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